Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQB67_RS11750 Genome accession   NZ_LR135782
Coordinates   2305002..2306372 (-) Length   456 a.a.
NCBI ID   WP_002324461.1    Uniprot ID   A0AB37VSB1
Organism   Enterococcus faecium isolate E4239     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2300002..2311372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB67_RS11735 epsC 2301323..2301859 (-) 537 WP_002287446.1 serine O-acetyltransferase EpsC -
  EQB67_RS11740 gltX 2302181..2303641 (-) 1461 WP_002293095.1 glutamate--tRNA ligase -
  EQB67_RS11745 - 2303811..2304968 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  EQB67_RS11750 radA 2305002..2306372 (-) 1371 WP_002324461.1 DNA repair protein RadA Machinery gene
  EQB67_RS11755 - 2306435..2306962 (-) 528 WP_002287450.1 dUTP diphosphatase -
  EQB67_RS11760 proB 2307192..2307998 (+) 807 WP_002324460.1 glutamate 5-kinase -
  EQB67_RS11765 - 2307995..2309242 (+) 1248 WP_002298218.1 glutamate-5-semialdehyde dehydrogenase -
  EQB67_RS11770 - 2309318..2309662 (+) 345 WP_002324458.1 hypothetical protein -
  EQB67_RS11775 - 2309917..2310237 (-) 321 WP_002324457.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49979.66 Da        Isoelectric Point: 7.7416

>NTDB_id=1125801 EQB67_RS11750 WP_002324461.1 2305002..2306372(-) (radA) [Enterococcus faecium isolate E4239]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVTMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1125801 EQB67_RS11750 WP_002324461.1 2305002..2306372(-) (radA) [Enterococcus faecium isolate E4239]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACGGACCGCAGAACTCGGACAAGTTTGACAGGAG
AAAAAGCAAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGATCAAAACAAAATTAGAAGAACTGAAC
CGTGTGTTAGGCGGAGGTGTGGTGCCAGGGTCTATGGTCTTGATTGGTGGCGATCCAGGTATCGGGAAATCCACTTTGCT
GTTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGAAAAGTTTTATATGTATCCGGCGAAGAAAGCGCAGAGCAGATAA
AATTACGCGCGGAACGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAACGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATCATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAACTACTCAAAATCGCTAAAACAAATGGGATAGCCATTTTTATCG
TCGGTCATGTTACCAAAGAAGGCTCGATCGCCGGCCCAAGAATGTTAGAACATATGGTGGATACGGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTTCGGATTTTGCGAGCAGTAAAAAACCGATTTGGCTCAACGAATGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAATCCTTCACAGATTTTCTTAGAAGAACGCCTAGACGGTGCAACTGGCT
CTGCTATCGTTGTGACAATGGAAGGGACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCCAAACGAACAACAACAGGTCTTGATTTCAATCGGGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGGTT
GCTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTATAAAGAAAAAGGAACACAGCCAACGGAATGTTTCATCGGTGAAATTGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGAGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTATACCTACCTAA
AAACAACTTAGGTAACTGGGAAGCGCCAAAAGGAATCGAGATAGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Streptococcus mitis NCTC 12261

74.505

99.781

0.743
  radA Streptococcus mitis SK321

74.286

99.781

0.741
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667

Multiple sequence alignment