Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQB55_RS12985 Genome accession   NZ_LR135482
Coordinates   2531638..2533008 (-) Length   456 a.a.
NCBI ID   WP_127824074.1    Uniprot ID   -
Organism   Enterococcus faecium isolate E4456     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2526638..2538008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB55_RS12970 epsC 2527959..2528495 (-) 537 WP_002287446.1 serine O-acetyltransferase EpsC -
  EQB55_RS12975 gltX 2528817..2530277 (-) 1461 WP_002287447.1 glutamate--tRNA ligase -
  EQB55_RS12980 - 2530447..2531604 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  EQB55_RS12985 radA 2531638..2533008 (-) 1371 WP_127824074.1 DNA repair protein RadA Machinery gene
  EQB55_RS12990 - 2533071..2533598 (-) 528 WP_002287450.1 dUTP diphosphatase -
  EQB55_RS12995 proB 2533828..2534634 (+) 807 WP_002287451.1 glutamate 5-kinase -
  EQB55_RS13000 - 2534631..2535878 (+) 1248 WP_002287452.1 glutamate-5-semialdehyde dehydrogenase -
  EQB55_RS13005 - 2535954..2536298 (+) 345 WP_002287507.1 hypothetical protein -
  EQB55_RS13010 - 2536415..2536741 (-) 327 WP_002287453.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49937.58 Da        Isoelectric Point: 7.7416

>NTDB_id=1125725 EQB55_RS12985 WP_127824074.1 2531638..2533008(-) (radA) [Enterococcus faecium isolate E4456]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIATFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1125725 EQB55_RS12985 WP_127824074.1 2531638..2533008(-) (radA) [Enterococcus faecium isolate E4456]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACGGACCGCAGAACTCGGACAAGTTTGACAGGAG
AAAAAGCAAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGATCAAAACAAAATTAGAAGAACTGAAC
CGTGTGTTAGGCGGAGGTGTGGTGCCAGGGTCTATGGTCTTGATTGGTGGCGATCCAGGTATCGGGAAATCCACTTTGCT
GTTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGAAAAGTCTTATATGTATCCGGCGAAGAAAGCGCAGAGCAGATAA
AATTACGCGCGGAACGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAACGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATCATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAACTACTCAAAATCGCTAAAACAAATGGGATAGCCACTTTTATCG
TCGGTCATGTTACCAAAGAAGGCTCGATCGCCGGCCCAAGAATGTTAGAACATATGGTGGATACGGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTTCGGATTTTGCGAGCAGTAAAAAACCGATTTGGCTCAACGAATGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAATCCTTCACAGATTTTCTTAGAAGAACGCCTAGACGGTGCAACTGGCT
CTGCTATCGTTGTGGCAATGGAAGGGACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCCAAACGAACAACAACAGGTCTTGATTTCAATCGGGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGGTT
GCTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTATAAAGAAAAAGGAACACAGCCAACGGAATGTTTCATCGGTGAAATTGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGAGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTATACCTACCTAA
AAACAACTTAGGTAACTGGGAAGCGCCAAAAGGAATCGAGATAGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(69-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Streptococcus mitis SK321

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664

Multiple sequence alignment