Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CJ8421_RS05710 Genome accession   NZ_CP005388
Coordinates   1081226..1082209 (+) Length   327 a.a.
NCBI ID   WP_032592455.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni CG8421     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1059874..1094404 1081226..1082209 within 0


Gene organization within MGE regions


Location: 1059874..1094404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ8421_RS05595 (CJ8421_05605) pglI 1059874..1060803 (-) 930 WP_002860218.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CJ8421_RS05600 (CJ8421_05610) pglH 1060796..1061875 (-) 1080 WP_002860219.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CJ8421_RS05605 (CJ8421_05615) pglK 1061872..1063566 (-) 1695 WP_038400581.1 ABC-type lipopolysaccharide transporter PglK -
  CJ8421_RS05610 (CJ8421_05620) galE 1063560..1064546 (-) 987 WP_032587260.1 UDP-glucose 4-epimerase GalE -
  CJ8421_RS05615 (CJ8421_05625) - 1064605..1065399 (-) 795 WP_002860221.1 3'-5' exonuclease -
  CJ8421_RS05620 (CJ8421_05630) waaC 1065466..1066494 (+) 1029 WP_002860222.1 lipopolysaccharide heptosyltransferase I -
  CJ8421_RS05625 (CJ8421_05635) htrB 1066484..1067362 (+) 879 WP_032592451.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CJ8421_RS05630 (CJ8421_05640) - 1067359..1068060 (+) 702 WP_002876132.1 glycosyltransferase family 2 protein -
  CJ8421_RS05635 (CJ8421_05645) rfbA 1068071..1068949 (+) 879 WP_032592508.1 glucose-1-phosphate thymidylyltransferase RfbA -
  CJ8421_RS05640 (CJ8421_05650) rfbB 1068949..1069980 (+) 1032 WP_002860226.1 dTDP-glucose 4,6-dehydratase -
  CJ8421_RS05645 (CJ8421_05655) wlaRA 1069983..1070408 (+) 426 WP_002860227.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  CJ8421_RS05650 (CJ8421_05660) wlaRB 1070430..1070834 (+) 405 WP_002860228.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  CJ8421_RS05655 (CJ8421_05665) - 1070824..1071267 (+) 444 WP_002860229.1 acyltransferase -
  CJ8421_RS05660 (CJ8421_05670) - 1071289..1071816 (+) 528 WP_002876185.1 GNAT family N-acetyltransferase -
  CJ8421_RS05665 (CJ8421_05675) - 1072113..1073177 (+) 1065 WP_002869599.1 glycosyltransferase family A protein -
  CJ8421_RS05670 (CJ8421_05680) - 1073190..1074242 (+) 1053 WP_002860232.1 glycosyltransferase family 2 protein -
  CJ8421_RS05675 (CJ8421_05685) wlaRG 1074208..1075293 (+) 1086 WP_038400589.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  CJ8421_RS05680 (CJ8421_05690) - 1075320..1076579 (-) 1260 WP_002869603.1 DUF2972 domain-containing protein -
  CJ8421_RS05685 - 1076576..1077597 (-) 1022 Protein_1093 hypothetical protein -
  CJ8421_RS05690 (CJ8421_05705) - 1077668..1078441 (+) 774 WP_002860236.1 glycosyltransferase family 25 protein -
  CJ8421_RS05695 (CJ8421_05710) wlaTB 1078434..1079390 (+) 957 WP_002860237.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  CJ8421_RS05700 (CJ8421_05715) wlaTC 1079387..1080346 (+) 960 WP_002860238.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  CJ8421_RS05705 (CJ8421_05720) - 1080349..1081167 (-) 819 WP_032592453.1 glycosyltransferase family 2 protein -
  CJ8421_RS05710 (CJ8421_05725) waaF 1081226..1082209 (+) 984 WP_032592455.1 lipopolysaccharide heptosyltransferase II Regulator
  CJ8421_RS05715 (CJ8421_05730) gmhA 1082162..1082725 (-) 564 WP_002860241.1 D-sedoheptulose 7-phosphate isomerase -
  CJ8421_RS05720 (CJ8421_05735) rfaE1 1082722..1084107 (-) 1386 WP_002901184.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CJ8421_RS05725 (CJ8421_05740) rfaD 1084100..1085053 (-) 954 WP_002860242.1 ADP-glyceromanno-heptose 6-epimerase -
  CJ8421_RS05730 (CJ8421_05745) gmhB 1085054..1085615 (-) 562 Protein_1102 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CJ8421_RS05735 (CJ8421_05750) - 1085696..1085998 (+) 303 WP_002852762.1 cytochrome c -
  CJ8421_RS05740 (CJ8421_05755) ccoS 1086021..1086227 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  CJ8421_RS05745 (CJ8421_05760) - 1086224..1088581 (-) 2358 WP_002860244.1 heavy metal translocating P-type ATPase -
  CJ8421_RS05750 (CJ8421_05765) rho 1088690..1089988 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  CJ8421_RS05755 (CJ8421_05770) dnaX 1089992..1091521 (+) 1530 WP_002860245.1 DNA polymerase III subunit gamma/tau -
  CJ8421_RS05760 (CJ8421_05775) - 1091518..1092039 (-) 522 WP_002853472.1 DUF2165 family protein -
  CJ8421_RS05765 (CJ8421_05780) copA 1092111..1094210 (-) 2100 WP_002860246.1 copper-translocating P-type ATPase CopA -
  CJ8421_RS05770 (CJ8421_05785) - 1094210..1094404 (-) 195 WP_002852760.1 cation transporter -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 37493.15 Da        Isoelectric Point: 10.0592

>NTDB_id=112563 CJ8421_RS05710 WP_032592455.1 1081226..1082209(+) (waaF) [Campylobacter jejuni subsp. jejuni CG8421]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKLQDPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKSEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAILVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKTLIDD
LTKCVYD

Nucleotide


Download         Length: 984 bp        

>NTDB_id=112563 CJ8421_RS05710 WP_032592455.1 1081226..1082209(+) (waaF) [Campylobacter jejuni subsp. jejuni CG8421]
ATGAAAATTTTTATACATCTTCCCACTTGGCTGGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAACTTCAAGACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAATCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAATCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAACAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAACGCAATATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAACTCTCATCGATGAT
CTGACAAAGTGTGTGTATGATTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.968

96.33

0.905

Multiple sequence alignment