Detailed information NTG1124536

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: EQB60_RS11700
• Genome accession: NZ_LR135219
• Coordinates: 2272135..2273943 (-)
• Length: 602 a.a.
• NCBI ID: WP_002288947.1
• Uniprot ID: A0A132ZEU6
• Organism: Enterococcus faecium isolate E7040
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2270635..2271930	2272135..2273943	flank	205

Gene organization within MGE regions

Location: 2270635..2273943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQB60_RS11695	-	2270635..2271930 (-)	1296	WP_002296623.1	ISL3 family transposase	-
EQB60_RS11700	pepF	2272135..2273943 (-)	1809	WP_002288947.1	oligoendopeptidase F	Regulator

Sequence

Protein

• Length: 602 a.a.
• Molecular weight: 69702.99 Da
• Isoelectric Point: 4.6502

>NTDB_id=1124536 EQB60_RS11700 WP_002288947.1 2272135..2273943(-) (pepF) [Enterococcus faecium isolate E7040]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQSEKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAH
LKNDQDTGNTDYQALYARASSLFSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAAFKGLYSVYEQFRNTFASTLGTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVDVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide

• Length: 1809 bp

>NTDB_id=1124536 EQB60_RS11700 WP_002288947.1 2272135..2273943(-) (pepF) [Enterococcus faecium isolate E7040]
ATGGAAGTAAAGCAGTTGCCAAAACGAGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAGTTTGATGAGAAATATTTGGAATTATCAGAAGAGTTAAAACAATCTGAAAAACACAAAGGAACACTTGATCAAG
GCGCTTCTCAATTTTTAAATGCGATTGAATTCGTATTGAGGGTTTATCGCCAAACTGAAGTCATTTATGTATATGCGCAC
CTTAAAAACGATCAAGACACTGGAAATACAGATTACCAAGCGCTTTATGCAAGAGCAAGCAGTCTGTTTTCGAAAGTTAG
TGAAGCCGTTTCCTGGTTTGAACCAGAAATATTGCAATTGTCAGATGACCAGATTTGGCAATATTTCAAAGAAGAACCAA
AATTGGAAGTCTATCGCCATTATATCCAGCAAATAGTAGATAATCGAGCCCATGTCTTATCTGCTGAGCAGGAATCTCTT
CTTGCTGGAGCAGGTGAAATCTTTGATGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATCTAGTTTTTCCAAC
GATTGAAGGAGAAAATGGTGAAATAGTCCAATTATCTCATGGCGTGTATGGTCAGTTGCTAGAAAGCACGGATCGAAGGG
TGCGCGAAGCAGCATTTAAGGGGTTGTACAGTGTTTACGAACAATTTAGAAATACATTTGCTTCTACTTTAGGCACACAT
ATAAAAGGACATAATTTTAAAGCGAAAGTCCGTAATTACAGCTCTGCCAGAGAAGCGTCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGATACTTTGGTAGACGTGGTAAACAAGCATTTGCCTTTGTTACATCGATACATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTATGATCTTTATACACCGGTCTTAGGGGAAGCTCCAATTACCTTTACGTAC
GAAGAAGCAAAAGAAAAAGCTTTAGAAGCACTGAAACCAATGGGTGAAGAATACATGGCCATCGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGGAAACGAAGCGGTGCTTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACGCTGGATCAGCTATTTACGCTTGTCCACGAAATGGGACATAGTGTTCATAGTTAT
TTCACTCGTTCGAACCAGCCTTATGTGTACGGCGACTACTCCATCTTTTTAGCAGAAATTGCTTCGACAACGAATGAAAA
TATCCTAACGGAGTATTTATTGGAAACAGAAAAAGACCCTCGTGTACGGGCTTATGTACTCAACCATTACTTGGATGGGT
TTAAAGGAACAGTTTTCCGCCAGACACAATTCGCTGAATTCGAGCATTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATACCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCAGCAGTCGAAGAAGACCCAGA
AATTAAATTCGAATGGTCACGGATACCGCATTTTTATTATAATTACTATGTTTTCCAATACTCGACTGGCTTTTCTGCTG
CTTCAGCACTGGCGAAGAAAATACTAAACCAGGAACCAGAGGCACTAGAGAACTACTTGGCTTACCTAAAAGCAGGTAAC
AGCGATTATCCTGTGGAAGTAATGAAAAAAGCAGGAGTTGATATGACACAGGCTGCATATATTGAAGATGCAATGTCGAT
GTTTGAACAACGTCTAAATGAATTAGAAGAATTGATCGATCGTTTATAA

Domains

Predicted by InterproScan.

• Oligopeptidase F (Peptidase_M3_N) (116-185)

• Peptidase family M3 (Peptidase_M3) (206-586)

3D structure

• AlphaFold DB: A0A132ZEU6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	55.649	98.505	0.548