Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EL354_RS12165 Genome accession   NZ_LR135191
Coordinates   2373873..2375681 (-) Length   602 a.a.
NCBI ID   WP_002323519.1    Uniprot ID   -
Organism   Enterococcus faecium isolate E4438     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2368873..2380681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL354_RS12140 - 2369913..2370812 (-) 900 WP_002294562.1 RluA family pseudouridine synthase -
  EL354_RS12145 - 2370814..2371611 (-) 798 WP_002323518.1 NAD kinase -
  EL354_RS12150 - 2371589..2372293 (-) 705 WP_002289848.1 GTP pyrophosphokinase family protein -
  EL354_RS12155 - 2372424..2372999 (+) 576 WP_002289849.1 CYTH domain-containing protein -
  EL354_RS12160 - 2373117..2373794 (+) 678 WP_002289850.1 ClpXP adapter SpxH family protein -
  EL354_RS12165 pepF 2373873..2375681 (-) 1809 WP_002323519.1 oligoendopeptidase F Regulator
  EL354_RS12170 - 2375857..2376855 (-) 999 WP_002288958.1 competence protein CoiA -
  EL354_RS12175 - 2377081..2377734 (-) 654 WP_002288948.1 adaptor protein MecA -
  EL354_RS12180 spxA 2378066..2378464 (-) 399 WP_002296384.1 transcriptional regulator SpxA -
  EL354_RS12185 - 2379229..2379936 (+) 708 WP_002294563.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69692.95 Da        Isoelectric Point: 4.6502

>NTDB_id=1124425 EL354_RS12165 WP_002323519.1 2373873..2375681(-) (pepF) [Enterococcus faecium isolate E4438]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQSEKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAH
LKNDQDTGNTDYQALYARASSLFSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAAFKGLYSVYEQFRNTFASTLGTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVDVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTSVLGEAPITFTY
EEAKEKALEALKPMGEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=1124425 EL354_RS12165 WP_002323519.1 2373873..2375681(-) (pepF) [Enterococcus faecium isolate E4438]
ATGGAAGTAAAGCAGTTGCCAAAACGAGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAGTTTGATGAGAAATATTTGGAATTATCAGAAGAGTTAAAACAATCTGAAAAACACAAAGGAACACTTGATCAAG
GCGCTTCTCAATTTTTAAATGCGATTGAATTCGTATTGAGGGTTTATCGCCAAACTGAAGTCATTTATGTATATGCGCAC
CTTAAAAACGATCAAGACACTGGAAATACAGATTACCAAGCGCTTTATGCAAGAGCAAGCAGTCTGTTTTCGAAAGTTAG
TGAAGCCGTTTCCTGGTTTGAACCAGAAATATTGCAATTGTCAGATGACCAGATTTGGCAATATTTCAAAGAAGAACCAA
AATTGGAAGTCTATCGCCATTATATCCAGCAAATAGTAGATAATCGAGCCCATGTCTTATCTGCTGAGCAGGAATCTCTT
CTTGCTGGAGCAGGTGAAATCTTTGATGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATCTAGTTTTTCCAAC
GATTGAAGGAGAAAATGGTGAAATAGTCCAATTATCTCATGGCGTGTATGGTCAGTTGCTAGAAAGCACGGATCGAAGGG
TGCGCGAAGCAGCATTTAAGGGATTGTACAGTGTTTACGAACAATTTAGAAATACATTTGCTTCTACTTTAGGCACACAT
ATAAAAGGACATAATTTTAAAGCGAAAGTCCGTAATTACAGCTCTGCCAGAGAAGCGTCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGATACTTTGGTAGACGTGGTAAACAAGCATTTGCCTTTGTTACATCGATACATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTATGATCTTTATACATCGGTCTTAGGGGAAGCTCCAATTACCTTTACGTAC
GAAGAAGCAAAAGAAAAAGCTTTAGAAGCACTGAAACCAATGGGTGAAGAATACATGGCCATCGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGGAAACGAAGCGGTGCTTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACGCTGGATCAGCTATTTACGCTTGTCCACGAAATGGGACATAGTGTTCATAGTTAT
TTCACTCGTTCGAACCAGCCTTATGTGTACGGCGACTACTCCATCTTTTTAGCAGAAATTGCTTCGACAACGAATGAAAA
TATCCTAACGGAGTATTTATTGGAAACAGAAAAAGACCCTCGTGTACGGGCTTATGTACTCAACCATTACTTGGATGGGT
TTAAAGGAACAGTTTTCCGCCAGACACAATTCGCTGAATTCGAGCATTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATACCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCAGCAGTCGAAGAAGACCCAGA
AATTAAATTCGAATGGTCACGGATACCGCATTTTTATTATAATTACTATGTTTTCCAATACTCGACTGGCTTTTCTGCTG
CTTCAGCACTGGCGAAGAAAATACTAAACCAGGAACCAGAGGCACTAGAGAACTACTTGGCTTACCTAAAAGCAGGTAAC
AGCGATTATCCTGTGGAAGTAATGAAAAAAGCAGGAGTTGATATGACACAGGCTGCATATATTGAAGATGCAATGTCGAT
GTTTGAACAACGTCTAAATGAATTAGAAGAATTGATCGATCGTTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(116-185)

Peptidase family M3 (Peptidase_M3)

(206-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.481

98.505

0.547

Multiple sequence alignment