Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   EL323_RS06310 Genome accession   NZ_LR134526
Coordinates   1089499..1090452 (-) Length   317 a.a.
NCBI ID   WP_002259883.1    Uniprot ID   -
Organism   Neisseria meningitidis strain NCTC10026     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1051695..1093475 1089499..1090452 within 0


Gene organization within MGE regions


Location: 1051695..1093475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL323_RS06105 (NCTC10026_01018) - 1051695..1053920 (+) 2226 WP_080542246.1 NADP-dependent isocitrate dehydrogenase -
  EL323_RS06110 (NCTC10026_01019) - 1054063..1054371 (+) 309 WP_002251257.1 hypothetical protein -
  EL323_RS14500 - 1054361..1054513 (+) 153 WP_014575291.1 hypothetical protein -
  EL323_RS15210 (NCTC10026_01021) - 1054991..1055122 (+) 132 WP_080542248.1 hypothetical protein -
  EL323_RS06125 (NCTC10026_01022) - 1055119..1055487 (+) 369 WP_080542249.1 type II toxin-antitoxin system death-on-curing family toxin -
  EL323_RS06135 (NCTC10026_01023) - 1055926..1056417 (+) 492 WP_002217437.1 DUF4760 domain-containing protein -
  EL323_RS06140 (NCTC10026_01024) - 1056776..1057012 (+) 237 WP_002217439.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  EL323_RS06145 (NCTC10026_01025) - 1057014..1057361 (+) 348 WP_080542251.1 type II toxin-antitoxin system PemK/MazF family toxin -
  EL323_RS06150 (NCTC10026_01026) - 1057414..1057923 (-) 510 WP_002219426.1 hypothetical protein -
  EL323_RS06155 (NCTC10026_01027) - 1057935..1058408 (-) 474 WP_080542252.1 D-Ala-D-Ala carboxypeptidase family metallohydrolase -
  EL323_RS06160 (NCTC10026_01028) - 1058472..1058621 (-) 150 WP_105184325.1 hypothetical protein -
  EL323_RS06165 (NCTC10026_01029) - 1058624..1059298 (-) 675 WP_002261808.1 hypothetical protein -
  EL323_RS06170 (NCTC10026_01030) - 1059295..1059702 (-) 408 WP_002256562.1 hypothetical protein -
  EL323_RS06175 (NCTC10026_01031) - 1059786..1060259 (-) 474 WP_080542253.1 hypothetical protein -
  EL323_RS06180 (NCTC10026_01032) - 1060317..1061612 (-) 1296 WP_080542254.1 DUF4043 family protein -
  EL323_RS14950 (NCTC10026_01033) - 1061633..1062604 (-) 972 WP_226876299.1 hypothetical protein -
  EL323_RS06190 (NCTC10026_01034) - 1062689..1063882 (-) 1194 WP_002238468.1 hypothetical protein -
  EL323_RS06195 (NCTC10026_01035) - 1063870..1067076 (-) 3207 WP_080624892.1 hypothetical protein -
  EL323_RS06200 (NCTC10026_01036) - 1067088..1067480 (-) 393 WP_002256557.1 hypothetical protein -
  EL323_RS06205 (NCTC10026_01037) - 1067480..1068379 (-) 900 WP_080542255.1 hypothetical protein -
  EL323_RS06210 (NCTC10026_01038) - 1068372..1068797 (-) 426 WP_002261801.1 hypothetical protein -
  EL323_RS06215 (NCTC10026_01039) - 1068813..1069220 (-) 408 WP_002261800.1 hypothetical protein -
  EL323_RS06220 (NCTC10026_01040) - 1069323..1078643 (-) 9321 WP_080542256.1 PLxRFG domain-containing protein -
  EL323_RS06225 (NCTC10026_01041) - 1078640..1079836 (-) 1197 WP_080542257.1 hypothetical protein -
  EL323_RS06230 (NCTC10026_01043) - 1080100..1081298 (-) 1199 Protein_1028 tyrosine-type recombinase/integrase -
  EL323_RS06245 (NCTC10026_01046) yaaA 1081829..1082608 (-) 780 WP_002245958.1 peroxide stress protein YaaA -
  EL323_RS15455 - 1082657..1082782 (-) 126 Protein_1030 IS5/IS1182 family transposase -
  EL323_RS06255 (NCTC10026_01047) dapC 1082870..1084057 (-) 1188 WP_080542258.1 succinyldiaminopimelate transaminase -
  EL323_RS06260 (NCTC10026_01048) dut 1084134..1084586 (-) 453 WP_002232309.1 dUTP diphosphatase -
  EL323_RS06265 (NCTC10026_01049) - 1084729..1085157 (+) 429 Protein_1033 AzlC family ABC transporter permease -
  EL323_RS06300 (NCTC10026_01050) pilX 1088449..1088922 (-) 474 WP_002230430.1 PilX family type IV pilin Machinery gene
  EL323_RS06305 (NCTC10026_01051) pilK 1088927..1089520 (-) 594 WP_002229321.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  EL323_RS06310 (NCTC10026_01052) pilJ 1089499..1090452 (-) 954 WP_002259883.1 PilW family protein Machinery gene
  EL323_RS06315 (NCTC10026_01053) pilV 1090449..1091069 (-) 621 WP_002253554.1 type IV pilus modification protein PilV Machinery gene
  EL323_RS06320 (NCTC10026_01054) pilH 1091099..1091767 (-) 669 WP_002259882.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  EL323_RS06330 (NCTC10026_01055) dnaB 1092069..1093475 (-) 1407 WP_002219448.1 replicative DNA helicase -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35030.78 Da        Isoelectric Point: 8.4318

>NTDB_id=1123971 EL323_RS06310 WP_002259883.1 1089499..1090452(-) (pilJ) [Neisseria meningitidis strain NCTC10026]
MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFTSRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFG
CFNMSEHIEQDVVSDVTQKDSLFSLKRNSTRNSTNKLIPIAESSNIGYQGFIQRLNALIFQYGIDDVNASADTTVVSSCA
AISKPGKQILNLEDVKKELKIVSQDKERNGNIARQRHVVNAYAVGRIAGEEGLFRFQLNEKGEWGNPQLLVKKINKMDIR
YIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL

Nucleotide


Download         Length: 954 bp        

>NTDB_id=1123971 EL323_RS06310 WP_002259883.1 1089499..1090452(-) (pilJ) [Neisseria meningitidis strain NCTC10026]
ATGAGACGTAAAATGCTAAACGTACCAAAAGGCAGTTATGATGGTATGAAAGGTTTTACCATTATTGAATTTTTGGTTGC
GGGCATGCTCAGTATGATTGTCCTGATGGCGGTCGGATCGAGTTACTTCACATCCCGGAAATTAAATGATGCGGCAAACG
AGCGTCTTGCTATTCAACAGGATTTGCGGAATGCGGCAACATTAATTGTCCGCGATGCAAGAATGGCGGGGAGCTTCGGT
TGTTTCAATATGTCTGAGCATATCGAACAGGATGTTGTTTCCGATGTGACGCAAAAAGACTCTCTTTTTTCCTTAAAAAG
GAACAGTACAAGGAACAGTACAAATAAACTTATCCCCATAGCGGAATCTTCAAATATCGGATATCAGGGTTTTATCCAGC
GCCTTAACGCATTGATTTTCCAATACGGAATCGATGATGTTAATGCAAGCGCCGATACTACCGTCGTCAGCAGCTGTGCC
GCAATATCGAAACCGGGTAAGCAAATCCTTAATTTAGAAGATGTAAAAAAAGAATTGAAGATTGTGAGTCAGGATAAGGA
GCGAAATGGCAATATAGCGCGTCAAAGGCATGTGGTCAATGCCTATGCGGTCGGCAGGATTGCCGGTGAGGAAGGTTTGT
TCCGCTTCCAATTGAATGAAAAGGGCGAGTGGGGTAATCCTCAGTTGTTGGTTAAAAAGATTAATAAAATGGATATACGG
TATATTTATGTTTCCGGCTGTCCTGAAGATGACGATGCCGGCAAAGAGGAAACATTCAGATATACGGATAAATTCAACAG
CGCCCAAGATGCTGTTACGCCTGCCGGGGTGGAGGTTTTATTGAGTAGCGGTACTGATACCAAGATTGCCGCTTCTTCAG
ACAATCATATTTATGCTTACCGTATCGATGCGACAATACGCGGGGGAAATGTATGCGCAAACAGAACACTTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Neisseria gonorrhoeae MS11

82.334

100

0.823

Multiple sequence alignment