Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL254_RS00820 Genome accession   NZ_LR134512
Coordinates   119077..120441 (+) Length   454 a.a.
NCBI ID   WP_001085195.1    Uniprot ID   A0AAV3JMW2
Organism   Streptococcus agalactiae strain NCTC13949     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 114077..125441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL254_RS00795 (NCTC13949_00159) rpoE 114787..115362 (+) 576 WP_000418426.1 DNA-directed RNA polymerase subunit delta -
  EL254_RS00800 (NCTC13949_00160) - 115635..117239 (+) 1605 WP_000170435.1 CTP synthase -
  EL254_RS00805 (NCTC13949_00161) - 117348..118274 (+) 927 WP_000723859.1 alpha/beta hydrolase -
  EL254_RS00815 (NCTC13949_00163) - 118469..118915 (+) 447 WP_000049184.1 dUTP diphosphatase -
  EL254_RS00820 (NCTC13949_00164) radA 119077..120441 (+) 1365 WP_001085195.1 DNA repair protein RadA Machinery gene
  EL254_RS00825 (NCTC13949_00165) - 120577..121074 (+) 498 WP_000214742.1 beta-class carbonic anhydrase -
  EL254_RS00830 (NCTC13949_00166) - 121228..122547 (+) 1320 WP_000759707.1 FAD-containing oxidoreductase -
  EL254_RS00835 (NCTC13949_00167) gltX 122756..124210 (+) 1455 WP_001284498.1 glutamate--tRNA ligase -
  EL254_RS00840 (NCTC13949_00168) - 124250..125218 (-) 969 WP_000668256.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49562.84 Da        Isoelectric Point: 6.5148

>NTDB_id=1123600 EL254_RS00820 WP_001085195.1 119077..120441(+) (radA) [Streptococcus agalactiae strain NCTC13949]
MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKNARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGEIGLTGEIR
RVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKAVFST

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1123600 EL254_RS00820 WP_001085195.1 119077..120441(+) (radA) [Streptococcus agalactiae strain NCTC13949]
GTGGCTAAGAAAAAATCAGTCTTCACCTGTCAAGAGTGTGGTTACCAATCACCTAAGTATTTGGGACGTTGTCCAAATTG
CTCAGCCTGGTCTTCATTTGTAGAAGAGGTTGAAGTTCAAGAAGTTAAAAATGCACGAGTTAGTTTGAACGGTGAAAAGT
CCCGACCGACGAAGTTAAAAGATGTTTCCTCAATTAACTATTCTAGAACTAAGACTGATATGGATGAATTTAACCGTGTT
CTAGGAGGCGGTGTTGTACCAGGAAGTTTGGTTCTGATTGGAGGAGACCCTGGGATAGGTAAATCTACTTTATTACTACA
AGTATCAACTCAGCTAGCTAATAAAGGTACAGTATTATATGTCTCAGGTGAGGAATCTGCAGAACAGATTAAGTTACGAA
GTGAGCGTCTAGGCGATATCGATAATGAATTTTACCTTTATGCCGAGACTAATATGCAAAGTATTCGGTCGGAGATTGAG
AAGATTAAACCAGATTTTCTTATTATTGATTCTATACAAACTATTATGAGTCCCGAAGTATCAAGTGTCCAAGGCTCTGT
TAGCCAAGTTCGCGAAGTGACCGCTGAATTGATGCAGTTAGCTAAAACTAATAATATTGCTACTTTTATTGTAGGTCATG
TGACTAAGGAGGGAACATTAGCAGGCCCTCGAATGCTAGAACATATGGTGGATACGGTTCTTTATTTTGAAGGGGAGCGT
CATCACACGTTCCGTATTTTACGGGCAGTCAAAAACCGTTTTGGTTCGACCAATGAAATAGGTATCTTTGAAATGCAGTC
GGGTGGTCTAGTTGAAGTTTTGAATCCAAGTCAAGTTTTTCTTGAAGAACGTTTAGACGGTGCAACAGGATCTGCTATTG
TTGTTACTATGGAAGGAACAAGGCCTATTTTAGCAGAGGTTCAAGCTTTAGTGACTCCGACTGTTTTTGGAAATGCTAAG
CGTACAACAACAGGTCTTGACTTTAATCGTGTCAGTCTTATTATGGCAGTTCTAGAAAAACGTTGTGGTTTATTGCTTCA
AAATCAAGATGCCTATTTAAAATCAGCGGGTGGTGTTAAATTGGATGAACCAGCAATTGATTTAGCTGTTGCTGTAGCTA
TTGCTTCCAGTTACAAAGAAAAACCCACAAATCCACAAGAATCATTTATTGGAGAAATTGGACTCACTGGTGAGATTCGT
CGCGTGACACGTATTGAGCAACGTATTAATGAGGCTAGTAAATTGGGCTTTACTAAAATTTACGCACCAAAGAATTCTCT
AGCTGGGATTGAAATACCTAAAGGCATTGATGTTATTGGTGTGACAACTGTTAGTCAGGTCTTAAAAGCAGTGTTTTCAA
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.079

99.78

0.879
  radA Streptococcus pneumoniae D39

88.079

99.78

0.879
  radA Streptococcus pneumoniae R6

88.079

99.78

0.879
  radA Streptococcus pneumoniae TIGR4

88.079

99.78

0.879
  radA Streptococcus mitis NCTC 12261

88.079

99.78

0.879
  radA Streptococcus mitis SK321

87.859

99.78

0.877
  radA Bacillus subtilis subsp. subtilis str. 168

62.914

99.78

0.628

Multiple sequence alignment