Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   EL227_RS05870 Genome accession   NZ_LR134499
Coordinates   1122950..1123909 (+) Length   319 a.a.
NCBI ID   WP_002856368.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NCTC13255     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1105267..1127952 1122950..1123909 within 0


Gene organization within MGE regions


Location: 1105267..1127952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL227_RS05785 (NCTC13255_01159) pglI 1105267..1106196 (-) 930 WP_002856262.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  EL227_RS05790 (NCTC13255_01160) pglH 1106189..1107268 (-) 1080 WP_002911763.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  EL227_RS05795 (NCTC13255_01161) pglK 1107265..1108959 (-) 1695 WP_002856016.1 ABC-type lipopolysaccharide transporter PglK -
  EL227_RS05800 (NCTC13255_01162) galE 1108953..1109939 (-) 987 WP_002856461.1 UDP-glucose 4-epimerase GalE -
  EL227_RS05805 (NCTC13255_01163) - 1109997..1110791 (-) 795 WP_002856546.1 3'-5' exonuclease -
  EL227_RS05810 (NCTC13255_01164) waaC 1110857..1111885 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  EL227_RS05815 (NCTC13255_01165) htrB 1111875..1112762 (+) 888 WP_126392528.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  EL227_RS05820 (NCTC13255_01166) - 1112759..1114307 (+) 1549 Protein_1120 glycosyltransferase -
  EL227_RS05825 (NCTC13255_01168) - 1114311..1115480 (+) 1170 WP_002852863.1 glycosyltransferase family 2 protein -
  EL227_RS05830 (NCTC13255_01169) - 1115550..1116593 (+) 1044 WP_002870498.1 beta-1,4-N-acetylgalactosaminyltransferase -
  EL227_RS05835 (NCTC13255_01170) - 1116579..1117484 (-) 906 WP_002852740.1 glycosyltransferase family 2 protein -
  EL227_RS05840 (NCTC13255_01171) - 1117573..1118448 (+) 876 WP_002869021.1 alpha-2,3-sialyltransferase -
  EL227_RS05845 (NCTC13255_01172) neuB 1118449..1119489 (+) 1041 WP_002870496.1 N-acetylneuraminate synthase -
  EL227_RS05850 (NCTC13255_01173) neuC 1119486..1120604 (+) 1119 WP_002911676.1 UDP-N-acetylglucosamine 2-epimerase -
  EL227_RS05855 (NCTC13255_01174) - 1120601..1121266 (+) 666 WP_002855897.1 acylneuraminate cytidylyltransferase family protein -
  EL227_RS05860 (NCTC13255_01175) - 1121254..1122087 (+) 834 WP_002855772.1 CatB-related O-acetyltransferase -
  EL227_RS05865 (NCTC13255_01176) waaV 1122079..1122891 (-) 813 WP_002856066.1 glycosyltransferase family 2 protein -
  EL227_RS05870 (NCTC13255_01177) waaF 1122950..1123909 (+) 960 WP_002856368.1 lipopolysaccharide heptosyltransferase II Regulator
  EL227_RS05875 (NCTC13255_01178) gmhA 1123890..1124450 (-) 561 WP_002855785.1 D-sedoheptulose 7-phosphate isomerase -
  EL227_RS05880 (NCTC13255_01179) rfaE1 1124447..1125832 (-) 1386 WP_002856251.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  EL227_RS05885 (NCTC13255_01180) rfaD 1125825..1126778 (-) 954 WP_002856483.1 ADP-glyceromanno-heptose 6-epimerase -
  EL227_RS05890 (NCTC13255_01181) gmhB 1126779..1127339 (-) 561 WP_002911679.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  EL227_RS05895 (NCTC13255_01182) - 1127421..1127723 (+) 303 WP_002852762.1 cytochrome c -
  EL227_RS05900 (NCTC13255_01183) ccoS 1127746..1127952 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36719.17 Da        Isoelectric Point: 10.1941

>NTDB_id=1123375 EL227_RS05870 WP_002856368.1 1122950..1123909(+) (waaF) [Campylobacter jejuni strain NCTC13255]
MKIFIHLPTWLGDAVMASPALYAIKEHFKDAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTNFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=1123375 EL227_RS05870 WP_002856368.1 1122950..1123909(+) (waaF) [Campylobacter jejuni strain NCTC13255]
ATGAAAATTTTTATACACCTTCCAACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
CTTTAAAGATGCCCAGTTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGTTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGACATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAATTTTACCCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTACATTTAGATCTAGCTTGCATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.611

100

0.956

Multiple sequence alignment