Detailed information NTG1123356

Bioinformatically predicted

Overview

• Name: waaF
• Type: Regulator
• Locus tag: EL232_RS07160
• Genome accession: NZ_LR134498
• Coordinates: 1319186..1320136 (-)
• Length: 316 a.a.
• NCBI ID: WP_126418116.1
• Uniprot ID: -
• Organism: Campylobacter jejuni strain NCTC13265
• Function: repress natural transformation (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	1315548..1341603	1319186..1320136	within	0

Gene organization within MGE regions

Location: 1315548..1341603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL232_RS07140 (NCTC13265_01445)	rfaD	1315548..1316501 (+)	954	WP_002873533.1	ADP-glyceromanno-heptose 6-epimerase	-
EL232_RS07145 (NCTC13265_01446)	rfaE1	1316494..1317879 (+)	1386	WP_126418114.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
EL232_RS07150 (NCTC13265_01447)	gmhA	1317876..1318436 (+)	561	WP_002842583.1	D-sedoheptulose 7-phosphate isomerase	-
EL232_RS07155 (NCTC13265_01448)	-	1318436..1319200 (+)	765	WP_002873535.1	glycosyltransferase family 25 protein	-
EL232_RS07160 (NCTC13265_01449)	waaF	1319186..1320136 (-)	951	WP_126418116.1	lipopolysaccharide heptosyltransferase II	Regulator
EL232_RS07165 (NCTC13265_01450)	-	1320195..1321016 (+)	822	WP_002872237.1	glycosyltransferase family 2 protein	-
EL232_RS07170 (NCTC13265_01451)	-	1321065..1322210 (+)	1146	WP_002872236.1	glycosyltransferase family 8 protein	-
EL232_RS07175 (NCTC13265_01452)	-	1322236..1323123 (+)	888	WP_002872235.1	glycosyltransferase family 2 protein	-
EL232_RS07180 (NCTC13265_01453)	-	1323092..1324642 (-)	1551	WP_126418118.1	glycosyltransferase	-
EL232_RS07185 (NCTC13265_01454)	htrB	1324639..1325526 (-)	888	WP_002867215.1	lipid A biosynthesis lauroyl acyltransferase HtrB	-
EL232_RS07190 (NCTC13265_01455)	waaC	1325516..1326544 (-)	1029	WP_011049885.1	lipopolysaccharide heptosyltransferase I	-
EL232_RS07195 (NCTC13265_01456)	-	1326611..1327405 (+)	795	WP_002860221.1	3'-5' exonuclease	-
EL232_RS07200 (NCTC13265_01457)	galE	1327464..1328450 (+)	987	WP_002867214.1	UDP-glucose 4-epimerase GalE	-
EL232_RS07205 (NCTC13265_01458)	pglK	1328444..1330138 (+)	1695	WP_002867213.1	ABC-type lipopolysaccharide transporter PglK	-
EL232_RS07210 (NCTC13265_01459)	pglH	1330135..1331214 (+)	1080	WP_002859237.1	GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase	-
EL232_RS07215 (NCTC13265_01460)	pglI	1331207..1332136 (+)	930	WP_002867212.1	GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase	-
EL232_RS07220 (NCTC13265_01461)	pglJ	1332124..1333221 (+)	1098	WP_002867211.1	N-acetylgalactosamine-N, N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase	-
EL232_RS07225 (NCTC13265_01462)	pglB	1333225..1335366 (+)	2142	WP_002860216.1	undecaprenyl-diphosphooligosaccharide--protein glycotransferase	-
EL232_RS07230 (NCTC13265_01463)	pglA	1335376..1336506 (+)	1131	WP_011049883.1	N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase	-
EL232_RS07235 (NCTC13265_01464)	pglC	1336499..1337101 (+)	603	WP_002867208.1	undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase	-
EL232_RS07240 (NCTC13265_01465)	pglD	1337088..1337678 (+)	591	WP_004308598.1	UDP-N-acetylbacillosamine N-acetyltransferase	-
EL232_RS07245 (NCTC13265_01466)	pglE	1337776..1338936 (+)	1161	WP_002852877.1	UDP-N-acetylbacillosamine transaminase	-
EL232_RS07250 (NCTC13265_01467)	pglF	1338938..1340710 (+)	1773	WP_002858054.1	UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining)	-
EL232_RS07255 (NCTC13265_01468)	pglG	1340710..1341603 (+)	894	WP_002856519.1	PDC sensor domain-containing protein	-

Sequence

Protein

• Length: 316 a.a.
• Molecular weight: 36293.86 Da
• Isoelectric Point: 10.3316

>NTDB_id=1123356 EL232_RS07160 WP_126418116.1 1319186..1320136(-) (waaF) [Campylobacter jejuni strain NCTC13265]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLGIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNQNARWVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLTNI

Nucleotide

• Length: 951 bp

>NTDB_id=1123356 EL232_RS07160 WP_126418116.1 1319186..1320136(-) (waaF) [Campylobacter jejuni strain NCTC13265]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACGAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGGCATAAAAGCTCATTTTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCACGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATCAAAATGCAAGATGGGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAACCTGAAGTGATTTTAAAGGCAATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

• Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9) (96-294)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
waaF	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	93.291	99.051	0.924