Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL199_RS04600 Genome accession   NZ_LR134365
Coordinates   956849..958225 (-) Length   458 a.a.
NCBI ID   WP_004139196.1    Uniprot ID   C8N6U8
Organism   Cardiobacterium hominis strain NCTC10426     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 951849..963225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS12835 (NCTC10426_00885) - 952420..952767 (-) 348 WP_170166214.1 DUF4189 domain-containing protein -
  EL199_RS04580 (NCTC10426_00886) - 952924..953682 (+) 759 WP_004139188.1 nucleotidyltransferase domain-containing protein -
  EL199_RS04585 (NCTC10426_00887) - 953676..954731 (+) 1056 WP_004139191.1 DNA polymerase beta superfamily protein -
  EL199_RS04590 (NCTC10426_00888) hemH 954852..955847 (-) 996 WP_004139193.1 ferrochelatase -
  EL199_RS04595 (NCTC10426_00889) galU 955959..956837 (-) 879 WP_004139194.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  EL199_RS04600 (NCTC10426_00890) radA 956849..958225 (-) 1377 WP_004139196.1 DNA repair protein RadA Machinery gene
  EL199_RS04605 (NCTC10426_00891) alr 958218..959285 (-) 1068 WP_040354412.1 alanine racemase -
  EL199_RS04610 (NCTC10426_00892) csm6 959353..960435 (-) 1083 WP_004139202.1 CRISPR-associated ring nuclease Csm6 -
  EL199_RS04615 (NCTC10426_00893) - 960450..960902 (-) 453 WP_004139204.1 hypothetical protein -
  EL199_RS04620 (NCTC10426_00894) - 960996..961385 (-) 390 WP_004139206.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48061.35 Da        Isoelectric Point: 7.7513

>NTDB_id=1122314 EL199_RS04600 WP_004139196.1 956849..958225(-) (radA) [Cardiobacterium hominis strain NCTC10426]
MAKAKSRFVCRDCGAAYPKWQGQCHECGAWNTLEEEAAPVAPPRAGKGGGYAGVAAGQVQRLDAVELQAETLVPSGISEF
DRVLGGGLVRGSVVLIGGDPGIGKSTLLLQTVHHLAQSMPCLYVSGEESPRQIGLRAERLGLARERIVLFSETQIERILA
TAAAEQPAVLVVDSIQTLYSEALSAAPGAVSQLRECAAQLVRFAKSSGTTVFLIGHVTKEGAIAGPRVLEHMVDTVLYFE
SEAGSRFRMLRAVKNRFGPVNELGMFAMLENGLKPVSNPSAIFLSRPDGQQPGSAVFPVWEGSRALLVEVQALVDGGGGG
YARRTALGFDSGRLAMLLAVLHRHGGVSLHDMDVYVNVVGGLKISEPAADLAVLAAAHSSLRGVAIPRDVVIMGEVGLAG
ELRPVANGEARIKAAAQHGFKQVILPEGNKPRGSATMRGLQIHAVATLAAALDVLSSL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1122314 EL199_RS04600 WP_004139196.1 956849..958225(-) (radA) [Cardiobacterium hominis strain NCTC10426]
ATGGCTAAGGCGAAGTCGCGTTTTGTCTGCCGCGATTGTGGTGCGGCGTATCCGAAATGGCAGGGGCAGTGCCACGAGTG
CGGCGCCTGGAACACGCTGGAGGAGGAAGCTGCGCCGGTCGCGCCGCCGCGCGCGGGCAAGGGCGGTGGTTATGCCGGGG
TTGCTGCCGGACAGGTGCAGCGGCTCGATGCGGTGGAATTGCAGGCGGAGACGCTGGTACCAAGCGGCATCAGCGAGTTT
GACCGCGTTCTCGGCGGCGGGCTGGTGCGCGGCAGCGTGGTGCTGATTGGCGGTGACCCGGGCATCGGCAAATCGACGCT
GTTGTTGCAGACGGTGCATCATTTGGCGCAGTCGATGCCGTGTTTGTATGTCAGCGGCGAGGAGTCGCCGCGCCAGATTG
GGCTGCGCGCCGAGCGGCTGGGGCTCGCCCGCGAGCGCATCGTGCTTTTCAGTGAAACGCAGATTGAGCGTATTTTGGCA
ACGGCCGCGGCGGAGCAGCCGGCGGTGCTGGTGGTCGATTCGATTCAGACGCTTTACAGTGAGGCGCTTTCTGCCGCGCC
GGGTGCGGTCAGCCAGTTGCGCGAGTGCGCAGCGCAGTTGGTGCGCTTTGCCAAGTCGAGCGGCACGACGGTCTTTCTCA
TCGGCCACGTTACCAAGGAGGGCGCGATTGCCGGCCCCCGCGTGCTGGAGCATATGGTCGATACGGTGCTGTATTTTGAA
AGCGAGGCGGGTAGCCGCTTCCGCATGCTGCGCGCGGTGAAAAACCGCTTCGGCCCGGTCAATGAGCTGGGGATGTTCGC
CATGCTGGAGAATGGCTTGAAGCCGGTGAGCAACCCGTCGGCGATTTTCCTCTCGCGCCCGGACGGGCAGCAGCCGGGCA
GCGCCGTTTTTCCGGTGTGGGAAGGCTCGCGCGCGCTCTTGGTCGAGGTGCAGGCGCTGGTTGATGGCGGCGGCGGTGGT
TATGCGCGCCGCACCGCGCTGGGTTTTGACAGCGGGCGGCTGGCGATGCTGCTCGCGGTGTTGCACCGCCACGGCGGCGT
CTCGCTGCACGACATGGACGTCTATGTGAACGTCGTCGGCGGCCTGAAAATCAGCGAACCGGCAGCAGATTTGGCGGTGC
TGGCGGCGGCGCATTCCAGCCTGCGCGGCGTTGCCATTCCGCGCGATGTGGTCATCATGGGCGAGGTCGGCCTGGCGGGT
GAACTGCGCCCGGTGGCGAACGGCGAGGCGCGCATCAAGGCGGCCGCGCAGCACGGTTTCAAACAGGTCATCTTGCCCGA
AGGGAACAAGCCGCGCGGCTCTGCTACAATGCGCGGCCTGCAAATTCACGCGGTTGCGACGCTCGCCGCCGCCCTTGACG
TACTCTCATCCCTATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(79-221)

Subunit ChlI of Mg-chelatase (ChlI)

(345-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8N6U8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.458

99.127

0.48
  radA Streptococcus pneumoniae Rx1

45.175

99.563

0.45
  radA Streptococcus pneumoniae D39

45.175

99.563

0.45
  radA Streptococcus pneumoniae TIGR4

45.175

99.563

0.45
  radA Streptococcus pneumoniae R6

45.175

99.563

0.45
  radA Streptococcus mitis SK321

45.175

99.563

0.45
  radA Streptococcus mitis NCTC 12261

44.956

99.563

0.448

Multiple sequence alignment