Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BTJ_RS10040 Genome accession   NZ_CP004117
Coordinates   2275708..2277216 (+) Length   502 a.a.
NCBI ID   WP_009893048.1    Uniprot ID   -
Organism   Burkholderia thailandensis E444     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270708..2282216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTJ_RS10015 (BTJ_1974) kdsD 2271939..2272922 (+) 984 WP_009893041.1 arabinose 5-phosphate isomerase KdsD -
  BTJ_RS10020 (BTJ_1975) - 2272922..2273461 (+) 540 WP_009893042.1 HAD family hydrolase -
  BTJ_RS10025 (BTJ_1976) lptC 2273466..2274080 (+) 615 WP_009893044.1 LPS export ABC transporter periplasmic protein LptC -
  BTJ_RS10030 (BTJ_1977) lptA 2274099..2274764 (+) 666 WP_009893045.1 lipopolysaccharide transport periplasmic protein LptA -
  BTJ_RS10035 (BTJ_1978) lptB 2274761..2275537 (+) 777 WP_009893047.1 LPS export ABC transporter ATP-binding protein -
  BTJ_RS10040 (BTJ_1979) rpoN/rpoN1 2275708..2277216 (+) 1509 WP_009893048.1 RNA polymerase factor sigma-54 Machinery gene
  BTJ_RS10045 (BTJ_1980) hpf 2277366..2277725 (+) 360 WP_009893049.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BTJ_RS10050 (BTJ_1981) - 2277958..2278467 (+) 510 WP_161654448.1 PTS sugar transporter subunit IIA -
  BTJ_RS10055 (BTJ_1982) hprK 2278596..2279564 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BTJ_RS10060 (BTJ_1983) rapZ 2279641..2280534 (+) 894 WP_009893058.1 RNase adapter RapZ -
  BTJ_RS10065 (BTJ_1984) - 2280561..2281193 (+) 633 WP_009893059.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54981.05 Da        Isoelectric Point: 4.8782

>NTDB_id=112203 BTJ_RS10040 WP_009893048.1 2275708..2277216(+) (rpoN/rpoN1) [Burkholderia thailandensis E444]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSSPEPMS
GASNGTTATNERTERDETAGVDEYDGYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=112203 BTJ_RS10040 WP_009893048.1 2275708..2277216(+) (rpoN/rpoN1) [Burkholderia thailandensis E444]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAATCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCAACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGACGACTGGA
TCGCGAGCCCCCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCTCGCCCGAGCCGATGTCC
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACGGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAATCTCGACGACTTCGGCCGCTCCCCCGCCGCATCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACCTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGACGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGGTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGGCTGGATCCGTCCCCGACGCGCACG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAACTGCTTGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTTCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAGCTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGTATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGTTCGAGACGATCCTGCGGGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACTCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGGTCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.504

100

0.693

Multiple sequence alignment