Detailed information NTG1122017

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: EL147_RS06895
• Genome accession: NZ_LR134341
• Coordinates: 1407803..1409176 (+)
• Length: 457 a.a.
• NCBI ID: WP_007892244.1
• Uniprot ID: -
• Organism: Streptococcus pseudoporcinus strain NCTC13786
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	1396390..1407776	1407803..1409176	flank	27

Gene organization within MGE regions

Location: 1396390..1409176

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL147_RS06805	-	1396390..1397832 (+)	1443	Protein_1266	IS1182 family transposase	-
EL147_RS06810 (NCTC13786_01366)	-	1398891..1399160 (-)	270	WP_007892295.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EL147_RS06815 (NCTC13786_01367)	relB	1399150..1399377 (-)	228	WP_007892538.1	type II toxin-antitoxin system RelB family antitoxin	-
EL147_RS06820 (NCTC13786_01369)	-	1399562..1399864 (-)	303	WP_007892306.1	hypothetical protein	-
EL147_RS06825 (NCTC13786_01370)	-	1399855..1400343 (-)	489	WP_176602242.1	hypothetical protein	-
EL147_RS06830 (NCTC13786_01371)	-	1400408..1400779 (-)	372	WP_232010994.1	hypothetical protein	-
EL147_RS06835	-	1400810..1401004 (-)	195	WP_007892513.1	DnaD domain protein	-
EL147_RS10725	-	1401188..1401337 (-)	150	Protein_1273	phage replisome organizer N-terminal domain-containing protein	-
EL147_RS06845 (NCTC13786_01373)	-	1401322..1401639 (-)	318	WP_007892369.1	hypothetical protein	-
EL147_RS06850 (NCTC13786_01374)	-	1401641..1401973 (-)	333	WP_007892612.1	hypothetical protein	-
EL147_RS06855 (NCTC13786_01376)	-	1402161..1402400 (-)	240	WP_007892248.1	hypothetical protein	-
EL147_RS06860	-	1402624..1403253 (-)	630	Protein_1277	Rha family transcriptional regulator	-
EL147_RS06865 (NCTC13786_01378)	-	1403265..1403450 (-)	186	WP_007892247.1	hypothetical protein	-
EL147_RS06870 (NCTC13786_01379)	-	1403617..1404165 (+)	549	WP_007892389.1	helix-turn-helix domain-containing protein	-
EL147_RS06875 (NCTC13786_01380)	-	1404584..1404859 (+)	276	WP_007892280.1	AlpA family transcriptional regulator	-
EL147_RS06880 (NCTC13786_01381)	-	1405035..1405997 (+)	963	WP_007892401.1	P63C domain-containing protein	-
EL147_RS06885	-	1406088..1407233 (+)	1146	Protein_1282	tyrosine-type recombinase/integrase	-
EL147_RS06890 (NCTC13786_01385)	-	1407330..1407776 (+)	447	WP_007892289.1	dUTP diphosphatase	-
EL147_RS06895 (NCTC13786_01386)	radA	1407803..1409176 (+)	1374	WP_007892244.1	DNA repair protein RadA	Machinery gene

Sequence

Protein

• Length: 457 a.a.
• Molecular weight: 50032.30 Da
• Isoelectric Point: 5.8264

>NTDB_id=1122017 EL147_RS06895 WP_007892244.1 1407803..1409176(+) (radA) [Streptococcus pseudoporcinus strain NCTC13786]
MAKKKATFVCQECGYHSPKYLGRCPNCSAWSSFVEEVEVQELKNARVSLTGEKSRPVKLKDVDNLNYSRTQTNMDEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGEIDNEFYLYAETNMQEIRIEIE
KIQPNFLIIDSIQTIMSPDSSGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTIAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVTNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKAAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYAPKNALQGLDISQNIEVVGLSTVGEVLKAVFTKNTK

Nucleotide

• Length: 1374 bp

>NTDB_id=1122017 EL147_RS06895 WP_007892244.1 1407803..1409176(+) (radA) [Streptococcus pseudoporcinus strain NCTC13786]
ATGGCTAAGAAAAAAGCAACATTTGTTTGTCAGGAATGTGGGTATCACTCTCCTAAATATTTAGGGCGTTGTCCCAACTG
CTCAGCCTGGTCTTCATTTGTGGAGGAAGTTGAAGTCCAAGAACTTAAAAATGCGCGTGTTAGCCTAACGGGTGAAAAAA
GTCGCCCGGTGAAACTAAAAGACGTTGATAACCTCAATTATTCCCGTACTCAGACCAACATGGATGAATTTAATCGAGTT
CTTGGCGGAGGTGTCGTTCCTGGCAGTCTTATCCTGATAGGAGGAGATCCTGGGATTGGTAAATCAACCTTACTCTTGCA
AGTTTCGACCCAGTTGGCCAATAAAGGAACTGTTTTATACGTTTCTGGGGAAGAATCAGCAGAACAAATTAAGTTGCGTA
GTGAACGTCTAGGTGAGATTGATAATGAATTTTATCTCTACGCCGAGACCAATATGCAAGAAATTAGAATTGAGATTGAG
AAAATTCAACCTAATTTTCTGATTATTGATTCTATTCAAACCATTATGAGTCCGGATAGTTCAGGTGTCCAAGGATCAGT
TAGTCAGGTAAGAGAAGTAACAGCGGAACTCATGCAATTAGCTAAGACTAATAATATTGCGACCTTTATAGTTGGACATG
TTACTAAAGAAGGAACCATAGCGGGTCCAAGAATGCTGGAGCATATGGTGGACACAGTTCTTTATTTTGAAGGGGAACGT
CATCATACCTTTAGGATTTTGCGAGCTGTTAAAAATCGTTTTGGATCAACTAATGAAATTGGTATTTTTGAAATGCAATC
AGGTGGTCTGGTTGAAGTTACTAATCCCAGTCAAGTTTTCCTAGAGGAACGCTTAGATGGGGCCACAGGCTCAGCTATCG
TTGTTACCATGGAAGGGAGTCGGCCAATTTTAGCCGAGGTCCAATCTTTAGTCACGCCAACTGTTTTTGGTAATGCCCGA
AGAACAACGACAGGGCTAGATTTCAATCGAGTTAGTTTAATTATGGCCGTTTTGGAAAAACGCTGTGGTTTGCTGTTACA
GAATCAGGATGCCTATCTGAAGGCCGCTGGAGGTGTCAAATTAGATGAGCCCGCTATTGACTTGGCTGTTGCAGTTGCCA
TTGCTTCAAGTTATAAGGAGTTGCCAACCAACCCTCAAGAAGCCTTTCTAGGAGAAATTGGTTTGACGGGTGAGATTAGA
CGGGTGACCCGCATTGAACAAAGGATTAATGAGGCAGCTAAGCTTGGCTTCACCAAGATTTATGCCCCTAAAAACGCCTT
ACAAGGGTTAGATATCTCTCAAAATATAGAAGTTGTTGGTCTATCAACTGTAGGTGAGGTTCTTAAAGCTGTCTTTACTA
AAAATACCAAGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (55-218)

• Subunit ChlI of Mg-chelatase (ChlI) (360-425)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus pneumoniae Rx1	84.956	98.906	0.84
radA	Streptococcus pneumoniae D39	84.956	98.906	0.84
radA	Streptococcus pneumoniae R6	84.956	98.906	0.84
radA	Streptococcus pneumoniae TIGR4	84.956	98.906	0.84
radA	Streptococcus mitis NCTC 12261	84.956	98.906	0.84
radA	Streptococcus mitis SK321	84.956	98.906	0.84
radA	Bacillus subtilis subsp. subtilis str. 168	60.486	99.125	0.6