Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL268_RS01350 Genome accession   NZ_LR134338
Coordinates   235089..236456 (+) Length   455 a.a.
NCBI ID   WP_088910268.1    Uniprot ID   A0A220MP74
Organism   Brevibacillus brevis strain NCTC2611     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 230089..241456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL268_RS01330 (NCTC2611_00274) - 230376..230840 (+) 465 WP_047074759.1 CtsR family transcriptional regulator -
  EL268_RS01335 (NCTC2611_00275) - 230857..231375 (+) 519 WP_106656584.1 UvrB/UvrC motif-containing protein -
  EL268_RS01340 (NCTC2611_00276) - 231395..232468 (+) 1074 WP_106656583.1 protein arginine kinase -
  EL268_RS01345 (NCTC2611_00277) - 232535..234985 (+) 2451 WP_016742864.1 ATP-dependent Clp protease ATP-binding subunit -
  EL268_RS01350 (NCTC2611_00278) radA 235089..236456 (+) 1368 WP_088910268.1 DNA repair protein RadA Machinery gene
  EL268_RS01355 (NCTC2611_00279) disA 236465..237541 (+) 1077 WP_106656588.1 DNA integrity scanning diadenylate cyclase DisA -
  EL268_RS01360 (NCTC2611_00280) pssA 237656..238384 (+) 729 WP_017247027.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  EL268_RS01365 (NCTC2611_00281) - 238481..238876 (-) 396 WP_007716195.1 hypothetical protein -
  EL268_RS01370 (NCTC2611_00282) - 239163..240251 (+) 1089 WP_106656582.1 PIN/TRAM domain-containing protein -
  EL268_RS01375 (NCTC2611_00283) ispD 240276..240962 (+) 687 WP_126435329.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EL268_RS01380 (NCTC2611_00284) ispF 240966..241439 (+) 474 WP_048035642.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49427.78 Da        Isoelectric Point: 6.8250

>NTDB_id=1121925 EL268_RS01350 WP_088910268.1 235089..236456(+) (radA) [Brevibacillus brevis strain NCTC2611]
MSKYKTKYACQECGYESPKWMGKCPGCSSWNTLVEEVTVKNAHRHEGLSGGQRQSAIPLTQVASEEEPRMDTTIGELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHQGAKVLYVSGEESAKQIKIRADRLHLQTPPMFVLAENDLDLIEQHI
NQVDPDVLIIDSIQTVFHPTIQSAAGSVAQVREATAQLMRIAKGKGIGTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVANPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRTATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPADIQVIGVSNIADALNEVIRR

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1121925 EL268_RS01350 WP_088910268.1 235089..236456(+) (radA) [Brevibacillus brevis strain NCTC2611]
ATGTCCAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCAAAATGGATGGGAAAATGCCCGGGATG
CAGCAGCTGGAACACTTTGGTAGAGGAAGTGACCGTAAAAAATGCACATCGTCACGAAGGGCTGAGCGGTGGCCAGCGTC
AATCTGCGATTCCATTGACTCAGGTAGCCAGTGAAGAAGAGCCACGCATGGATACGACGATCGGAGAATTGAATCGTGTT
CTTGGTGGAGGACTTGTTCCAGGTTCACTCATTTTGGTTGGGGGAGACCCCGGAATCGGAAAATCGACATTGCTTTTGCA
GACATCTTTTGCCTTGGCTCATCAAGGGGCGAAAGTTCTCTATGTATCTGGAGAGGAATCGGCAAAGCAAATCAAAATTC
GCGCGGATCGACTCCATTTGCAAACGCCGCCGATGTTTGTATTGGCAGAAAATGATTTGGATTTGATCGAACAGCATATT
AATCAAGTAGACCCGGATGTGCTAATTATTGACTCGATCCAGACCGTGTTTCACCCAACGATCCAATCGGCTGCTGGAAG
TGTCGCACAAGTGCGGGAGGCTACTGCCCAATTGATGCGGATTGCAAAAGGGAAAGGCATCGGTACCTTCATTGTCGGTC
ACGTTACCAAGGAAGGCTCCATCGCCGGTCCGCGTATGCTTGAGCATATGGTGGATGCTGTTCTTTATTTTGAAGGGGAG
CGCCACAATACGTTTCGAATTTTGCGCGCCGTCAAAAACCGCTTTGGTTCGACAAACGAAATCGGTATTTTCGAGATGAA
GGATCGTGGTTTGGAGGAAGTGGCGAATCCATCTGAGATATTTTTGGCAGAGCGTCCATTTGGTGTTGCGGGTTCGACAG
TCGTAGCCAGTATGGAGGGCACACGTCCCGTCTTGGTTGAGCTGCAAGCTCTCGTTGCCCCGACCAGCTTTGTAACCCCA
CGGAGAACGGCAACAGGGGTCGATCATCAGCGTGTGGCGATGATCATGGCAGTATTGGAAAAGCGCATGGGCATGATGCT
GCAAAATCAGGATGCATATGTAAACGTAGCAGGCGGGGTCAGATTGGATGAGCCGGCCGTTGATTTGGCAATTGCCGTAA
GCATTGCAAGCAGTTTCCGTGACCACGCTACCAATCCGCATGATGTCGTTATTGGAGAAATTGGCCTGACTGGTGAGGTG
CGGGGTGTCTCTCGTATTGAGCAGCGAGTACGAGAAGCACATAAATTAGGCTTTAAGCGTGTTATCATACCGGAGAAAAA
CATCCGCGGTCTGGATGCACCAGCAGATATTCAAGTAATTGGTGTGAGCAATATTGCCGATGCATTGAACGAGGTGATAA
GGAGGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-219)

Subunit ChlI of Mg-chelatase (ChlI)

(329-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220MP74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.929

99.341

0.655
  radA Streptococcus pneumoniae Rx1

58.057

99.56

0.578
  radA Streptococcus pneumoniae D39

58.057

99.56

0.578
  radA Streptococcus pneumoniae R6

58.057

99.56

0.578
  radA Streptococcus pneumoniae TIGR4

58.057

99.56

0.578
  radA Streptococcus mitis NCTC 12261

58.057

99.56

0.578
  radA Streptococcus mitis SK321

58.057

99.56

0.578

Multiple sequence alignment