Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EL154_RS10820 Genome accession   NZ_LR134337
Coordinates   2112606..2114414 (-) Length   602 a.a.
NCBI ID   WP_002327288.1    Uniprot ID   A0A2G0ECU4
Organism   Enterococcus faecium strain NCTC7174     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2107606..2119414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL154_RS10795 (NCTC7174_02114) - 2108646..2109545 (-) 900 WP_002327286.1 RluA family pseudouridine synthase -
  EL154_RS10800 (NCTC7174_02115) - 2109547..2110344 (-) 798 WP_002318543.1 NAD kinase -
  EL154_RS10805 (NCTC7174_02116) - 2110322..2111026 (-) 705 WP_002289848.1 GTP pyrophosphokinase family protein -
  EL154_RS10810 (NCTC7174_02117) - 2111157..2111732 (+) 576 WP_002289849.1 CYTH domain-containing protein -
  EL154_RS10815 (NCTC7174_02118) - 2111850..2112527 (+) 678 WP_002289850.1 ClpXP adapter SpxH family protein -
  EL154_RS10820 (NCTC7174_02119) pepF 2112606..2114414 (-) 1809 WP_002327288.1 oligoendopeptidase F Regulator
  EL154_RS10825 (NCTC7174_02120) - 2114635..2115588 (-) 954 WP_002327289.1 competence protein CoiA -
  EL154_RS10830 (NCTC7174_02121) - 2115815..2116468 (-) 654 WP_002299370.1 adaptor protein MecA -
  EL154_RS10835 (NCTC7174_02122) spxA 2116800..2117198 (-) 399 WP_002296384.1 transcriptional regulator SpxA -
  EL154_RS10840 (NCTC7174_02123) - 2117963..2118670 (+) 708 WP_002294563.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69660.95 Da        Isoelectric Point: 4.6724

>NTDB_id=1121908 EL154_RS10820 WP_002327288.1 2112606..2114414(-) (pepF) [Enterococcus faecium strain NCTC7174]
MEVKQLPKREELPGNLTWDLTKIFSSDQEFDEKYLELSEELKQSEKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAH
LKNDQDTGNTDYQALYARASSLFSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAAFKGLYSVYEQFRNTFASTLGTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVDVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNTKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=1121908 EL154_RS10820 WP_002327288.1 2112606..2114414(-) (pepF) [Enterococcus faecium strain NCTC7174]
ATGGAAGTAAAGCAGTTGCCAAAACGAGAAGAATTGCCTGGAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAGTTTGATGAGAAATATTTGGAATTATCAGAAGAGTTAAAACAATCTGAAAAACACAAAGGAACACTTGATCAAG
GCGCTTCTCAATTTTTAAATGCGATTGAATTCGTATTGAGGGTTTATCGCCAAACTGAAGTCATTTATGTATATGCGCAC
CTTAAAAACGATCAAGACACTGGAAATACAGATTACCAAGCGCTTTATGCAAGAGCAAGCAGTCTGTTTTCGAAAGTTAG
TGAAGCCGTTTCCTGGTTTGAACCAGAAATATTGCAATTGTCAGATGACCAGATTTGGCAATATTTCAAAGAAGAACCAA
AATTGGAAGTCTATCGCCATTATATCCAGCAAATAGTAGATAATCGAGCCCATGTCTTATCTGCTGAGCAGGAATCTCTT
CTTGCTGGAGCAGGTGAAATCTTTGATGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATCTAGTTTTTCCAAC
GATTGAAGGAGAAAATGGTGAAATAGTCCAATTATCTCATGGCGTGTATGGTCAGTTGCTAGAAAGCACGGATCGAAGGG
TACGCGAAGCAGCATTTAAGGGATTGTACAGTGTTTACGAACAATTTAGAAATACATTTGCTTCTACTTTAGGCACACAT
ATAAAAGGACATAATTTTAAAGCGAAAGTCCGTAATTACAGCTCTGCCAGAGAAGCGTCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGATACTTTGGTAGACGTGGTAAACAAGCATTTGCCTTTGTTACATCGATACATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTATGATCTTTATACACCGGTCTTAGGGGAAGCTCCAATTACCTTTACGTAC
GAAGAAGCAAAAGAAAAAGCTTTAGAAGCACTGAAACCAATGGGTGAAGAATACATGGCCATCGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGGAAACGAAGCGGTGCTTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACGCTGGATCAGCTATTTACGCTTGTCCACGAAATGGGACATAGTGTTCATAGTTAT
TTCACTCGTTCGAACCAGCCTTATGTGTACGGCGACTACTCCATCTTTTTAGCAGAAATTGCTTCGACAACGAATGAAAA
TATCCTAACGGAGTATTTATTGGAAACAGAAAAAGACCCTCGTGTACGGGCTTATGTACTCAACCATTACTTGGATGGGT
TTAAAGGAACAGTTTTCCGCCAGACACAATTCGCTGAATTCGAGCATTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATACCTAAGTGATAGTTATGGTAAATTGAATACAAAATATTATGGTCCAGCAGTCGAAGAAGACCCAGA
AATTAAATTCGAATGGTCACGGATACCGCATTTTTATTATAATTACTATGTTTTCCAATACTCGACTGGCTTTTCTGCTG
CTTCAGCACTGGCGAAGAAAATACTAAACCAGGAACCAGAGGCACTAGAGAACTACTTGGCTTACCTAAAAGCAGGTAAC
AGCGATTATCCTGTGGAAGTAATGAAAAAAGCAGGAGTTGATATGACACAGGCTGCATATATCGAAGATGCAATGTCGAT
GTTTGAACAACGTCTAAATGAATTAGAAGAATTGATCGATCGTTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(116-185)

Peptidase family M3 (Peptidase_M3)

(206-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G0ECU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.481

98.505

0.547

Multiple sequence alignment