Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   VV93_RS13540 Genome accession   NZ_CP009261
Coordinates   2940364..2942088 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain 93U204     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2935364..2947088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV93_RS13505 (VV93_v1c26600) - 2935704..2936393 (-) 690 WP_039554773.1 PilW family protein -
  VV93_RS13510 (VV93_v1c26610) - 2936393..2936959 (-) 567 WP_039554775.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV93_RS13515 (VV93_v1c26620) - 2936959..2937456 (-) 498 WP_039554777.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV93_RS13520 (VV93_v1c26630) - 2937508..2937978 (-) 471 WP_039554779.1 type II secretion system protein -
  VV93_RS13525 (VV93_v1c26640) - 2938012..2938590 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VV93_RS13530 (VV93_v1c26650) - 2938669..2939130 (-) 462 WP_039554781.1 hypothetical protein -
  VV93_RS13535 (VV93_v1c26660) - 2939134..2940354 (-) 1221 WP_011079398.1 type II secretion system F family protein -
  VV93_RS13540 (VV93_v1c26670) pilF 2940364..2942088 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  VV93_RS13545 (VV93_v1c26680) - 2942085..2943206 (-) 1122 WP_039554783.1 tetratricopeptide repeat protein -
  VV93_RS13550 (VV93_v1c26690) - 2943203..2944051 (-) 849 WP_039554785.1 ExeA family protein -
  VV93_RS13555 (VV93_v1c26700) mshL 2944059..2945705 (-) 1647 WP_072612424.1 pilus (MSHA type) biogenesis protein MshL -
  VV93_RS13560 (VV93_v1c26710) - 2945721..2946041 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  VV93_RS13565 (VV93_v1c26720) gspM 2946034..2946684 (-) 651 WP_039554789.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=112151 VV93_RS13540 WP_011079397.1 2940364..2942088(-) (pilF) [Vibrio vulnificus strain 93U204]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=112151 VV93_RS13540 WP_011079397.1 2940364..2942088(-) (pilF) [Vibrio vulnificus strain 93U204]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATAATCAATGAAGCGCAACTTGATCAAGC
TCTCGGCGCGCAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTGATTGACTTAAATCGAGCGACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCAGCGGACCTTTTTGCGCAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GACAATTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCCGAGCAGTTACAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGCGATGAAGTGACGGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGCGCATCGGATATCCATATCGAACCGGACGCCAATGTGTTGCGCTTGCGCCAAC
GTATTGATGGTGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGACATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACGGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CTACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTGTTGAGTACCTTGCACACCAATGACGCTGTAGACAGTGCGCTTCG
TATGATTGACATGGGCGCTCCGGGCTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACGGAAGATCATGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTTGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTATTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment