Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL257_RS23315 Genome accession   NZ_LR134318
Coordinates   5116536..5117903 (-) Length   455 a.a.
NCBI ID   WP_126366560.1    Uniprot ID   A0A3S4T3Y4
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5111536..5122903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS23290 (NCTC9428_04641) - 5111677..5112381 (+) 705 WP_126366551.1 GntR family transcriptional regulator -
  EL257_RS23295 (NCTC9428_04642) yjiA 5112495..5113472 (-) 978 WP_126366553.1 GTPase -
  EL257_RS23300 (NCTC9428_04643) - 5113625..5113822 (-) 198 WP_126366556.1 YbdD/YjiX family protein -
  EL257_RS23305 (NCTC9428_04644) - 5113838..5115904 (-) 2067 WP_016773099.1 carbon starvation CstA family protein -
  EL257_RS23310 (NCTC9428_04645) - 5116064..5116432 (+) 369 WP_126366558.1 PilZ domain-containing protein -
  EL257_RS23315 (NCTC9428_04646) radA 5116536..5117903 (-) 1368 WP_126366560.1 DNA repair protein RadA Machinery gene
  EL257_RS23320 (NCTC9428_04647) mscL 5118489..5118902 (+) 414 WP_126366562.1 large-conductance mechanosensitive channel protein MscL -
  EL257_RS23325 (NCTC9428_04648) - 5118946..5119722 (-) 777 WP_126366564.1 ferredoxin--NADP reductase -
  EL257_RS23330 (NCTC9428_04649) - 5120033..5120746 (+) 714 WP_126366566.1 autoinducer binding domain-containing protein -
  EL257_RS23335 (NCTC9428_04650) - 5120917..5122041 (+) 1125 WP_126366568.1 methyltransferase -
  EL257_RS23340 (NCTC9428_04651) - 5122112..5122279 (-) 168 WP_095048725.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48713.20 Da        Isoelectric Point: 6.8987

>NTDB_id=1121332 EL257_RS23315 WP_126366560.1 5116536..5117903(-) (radA) [Pseudomonas fluorescens strain NCTC9428]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMIESGGAAAPAGRTGWAGQQAQIKTLAEVSIEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1121332 EL257_RS23315 WP_126366560.1 5116536..5117903(-) (radA) [Pseudomonas fluorescens strain NCTC9428]
ATGGCCAAGGCCAAACGCATGTACGGCTGCACCGAGTGCGGCGCAACCTTTCCCAAATGGGCCGGGCAGTGCGGGGAGTG
CGGTGCCTGGAACACCCTGACCGAAACGATGATTGAAAGCGGTGGCGCCGCCGCACCGGCCGGGCGCACCGGCTGGGCTG
GGCAGCAAGCGCAGATCAAGACGCTGGCGGAAGTCAGCATTGAAGAAATCCCGCGATTTTCCACGGCGTCCGGCGAACTG
GATCGAGTACTCGGCGGTGGGCTGGTGGACGGTTCGGTGGTGTTGATTGGCGGCGATCCGGGCATCGGCAAGTCGACCAT
TCTTCTCCAGACCCTGTGCAACCTGGCCAAGAGCATGCCGGCGCTGTACGTCACCGGTGAAGAGTCCCAACAGCAAGTGG
CGATGCGCGCGCGTCGCCTGGGCTTGCCACAGGATCAACTGCGGGTGATGACCGAAACCTGCATCGAAACCATCATCGCC
ACTGCCCGTCAGGAAAAGCCCAAGGTGATGGTGATCGACTCGATCCAGACGATTTTCACCGAGCAGTTGCAATCGGCACC
GGGCGGCGTCTCCCAAGTGCGTGAGAGCGCCGCGTTGCTGGTGCGTTACGCCAAACAGAGCGGCACGGCGATTTTCCTCG
TCGGCCACGTCACCAAGGAGGGCGCACTGGCGGGGCCTCGTGTGCTGGAACACATGGTCGACACCGTGTTGTATTTCGAG
GGCGAATCCGATGGCCGCTTGCGTTTGCTGCGTGCGGTGAAAAACCGTTTCGGTGCGGTCAACGAGCTGGGCGTGTTCGG
CATGACTGACAAAGGCTTGAAAGAAGTCTCCAACCCCTCGGCAATTTTTCTGACGCGTGCGCAGGAAGAAGTCCCGGGCA
GTGTGGTGATGGCAACGTGGGAAGGCACGCGGCCGATGCTGGTGGAAGTGCAGGCGCTGGTCGATGACAGCCATCTGGCC
AACCCGCGCCGCGTAACACTGGGCCTGGATCAGAATCGTCTGGCGATGTTGCTTGCGGTTTTGCATCGTCACGGCGGTAT
TCCGACCCACGATCAGGACGTGTTTCTCAACGTGGTCGGCGGCGTGAAGGTGCTGGAGACGGCATCGGACCTGGCACTGA
TGGCGGCAGTCATGTCGAGCCTGCGCAACCGGCCGTTGCCGCATGATTTGCTGGTGTTTGGCGAGGTCGGTCTGTCGGGC
GAAGTGCGACCGGTGCCGAGTGGGCAGGAACGTTTGAAGGAAGCGGCCAAACACGGCTTCAAGCGCGCAATCGTGCCGAA
GGGAAATGCACCGAAGGAATCGCCGCCGGGGCTGCAGATCATCGCGGTCACCCGCCTTGAACAGGCCCTCGACGCCCTCT
TCGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(81-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4T3Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.672

100

0.499
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464
  radA Streptococcus pneumoniae Rx1

46.272

100

0.464
  radA Streptococcus pneumoniae D39

46.272

100

0.464
  radA Streptococcus pneumoniae R6

46.272

100

0.464
  radA Streptococcus pneumoniae TIGR4

46.272

100

0.464

Multiple sequence alignment