Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   EL257_RS01880 Genome accession   NZ_LR134318
Coordinates   412535..414754 (-) Length   739 a.a.
NCBI ID   WP_126359310.1    Uniprot ID   A0A448DKG7
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407535..419754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS01860 (NCTC9428_00377) hslU 407710..409047 (-) 1338 WP_126359304.1 ATP-dependent protease ATPase subunit HslU -
  EL257_RS01865 (NCTC9428_00378) hslV 409096..409632 (-) 537 WP_126359306.1 ATP-dependent protease subunit HslV -
  EL257_RS01870 (NCTC9428_00379) - 409852..410568 (-) 717 WP_122593939.1 SPOR domain-containing protein -
  EL257_RS01875 (NCTC9428_00380) argS 410570..412306 (-) 1737 WP_126359308.1 arginine--tRNA ligase -
  EL257_RS28180 - 412305..412427 (+) 123 WP_016772057.1 hypothetical protein -
  EL257_RS01880 (NCTC9428_00381) priA 412535..414754 (-) 2220 WP_126359310.1 primosomal protein N' Machinery gene
  EL257_RS01885 (NCTC9428_00382) rpmE 415038..415268 (+) 231 WP_126359313.1 50S ribosomal protein L31 -
  EL257_RS01890 (NCTC9428_00383) - 415283..416083 (+) 801 WP_126359315.1 thermonuclease family protein -
  EL257_RS01895 (NCTC9428_00384) - 416268..417536 (+) 1269 WP_126359317.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82069.46 Da        Isoelectric Point: 8.6679

>NTDB_id=1121312 EL257_RS01880 WP_126359310.1 412535..414754(-) (priA) [Pseudomonas fluorescens strain NCTC9428]
MPDAILRLALPSPLRRLFDYRAPAGVRREQLQPGMRLRVPFGRREMIGILVEVTDTSEVPAEKLKPALALLDDTPPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGELAEARQERFWSITPGASVDDPRVARAPRQREALTTLAQHPHGVAHQLL
SKLMLSKDSLDLLLAKGLVQVDIRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECSRCDARMTVHQRYGELRCHHCGNVERTPRQC
PKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGHPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGRVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDEACSAAERLLAEQNLSGIELLGPVPAPMERRAGRYRAQLLLQATARAPLHRLLASWLLVLEQ
MPSGRAVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=1121312 EL257_RS01880 WP_126359310.1 412535..414754(-) (priA) [Pseudomonas fluorescens strain NCTC9428]
GTGCCCGACGCCATTCTGCGCCTCGCCCTGCCTTCGCCTCTGCGCCGCCTGTTCGATTATCGAGCGCCGGCCGGGGTTCG
GCGCGAGCAATTGCAGCCGGGCATGCGCCTGCGGGTGCCGTTCGGGCGACGCGAGATGATCGGCATTCTGGTCGAGGTGA
CCGACACCAGCGAAGTGCCGGCGGAAAAGCTCAAACCGGCCCTGGCCCTGCTTGATGACACGCCGCCGCTGCCGCCAGCC
CTGTTCAAGCTGTGCCTGTGGACCGCGCAGTATTATCAGCACAGCCTCGGCGACACGTTGAGCTGGGCATTGCCGGTGCT
GTTGCGTCAGGGAGAACTGGCCGAAGCTCGCCAGGAGCGGTTCTGGTCGATTACGCCAGGAGCCAGCGTCGACGATCCAC
GGGTTGCGCGCGCGCCACGCCAACGCGAGGCGCTGACAACCCTGGCGCAGCATCCTCACGGCGTCGCCCATCAGTTGCTG
AGCAAACTGATGCTGAGCAAAGACAGCCTCGACCTGTTGCTGGCCAAGGGTTTGGTGCAAGTCGACATCCGTCGCCACGC
CCCCGGCGCGCGCCATGAACACTGGCTCGCCCAGCCCGAGCTGCCGCTCAACAGCGAGCAACGCGCCGCGTATGAAGCGA
TCCGCGCCGGATTCGACAGTTATCACGCCTTCCTGCTGGCCGGCGTCACGGGCAGCGGTAAGACCGAAGTCTATTTACAG
CTGATCCGCGAGACCCTCGAAGCCGGCAAGCAAGCGCTGGTGCTGATCCCCGAGATCAACCTTGGTCCGCAGACACTGGC
GCGTTTCGAACAGCGCTTCAACGCGCGCATAGCCCTGGTGCATTCAGCGGTCAATGATCGCGAACGCCTCGACGCCTGGC
TAGCCGCCCGCGATGGCGAGGCCGACATTATTATCGGCACCCGCTCGGCGCTGTTCACCCCGATGAAAAATCCCGGGCTG
ATTATCATCGACGAAGAGCACGATGGCTCTTATAAGCAGCAGGAAGGCTTGCGCTACCACGCCCGCGATCTGGCACTGGT
GCGCGCACGCCAGGAAAACATCCCGATTGTGCTGGGTTCCGCTACGCCGTCGCTGGAAAGCCTGCATAACGCCTACACCG
GACGCTACGGCCTGCTGCGCTTGAACGAACGCGCCGGCGGTGCCAAGCAGCCACGCTTCCTGCGCCTGGATGTGAAAAGT
CGCCCACTCGACAGCGGGATTTCCGGGCCGATGCAGCAAGCCATCGGCCAGACCCTCGCGGCCGGCCAGCAGGTGCTGGT
ATTCCTCAACCGTCGAGGATTTGCGCCGACGTTGCTGTGCCACGATTGCGGCTGGATGTCCGAATGCAGCCGCTGCGACG
CGCGGATGACCGTGCACCAGCGCTACGGCGAACTGCGCTGCCACCATTGCGGCAATGTCGAGCGCACCCCGCGCCAGTGC
CCGAAGTGCAACAAAGTCGATCTGCGCCCGGTCGGCGCTGGGACCGAGCGCGCGGAAGAACGCCTGGCGATTCTGTTCCC
TGATTACCCGGTGCTGCGCGTCGATCGCGACAGCACTTCACGCAAGGACGCGATGAATCAGCTGTTTGCAACGATTCAGA
AAGGCCATCCGTGCATTCTGGTCGGCACGCAAATGCTGGCCAAGGGGCACCATTTCCCACGGGTGACGCTGGTATCGATT
CTGGATGCCGACGGCGGCTTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAGTTGATCGTGCAGGTGGCGGG
ACGTGCCGGTCGGGCGGAAGAACCAGGCAGAGTGATCATCCAGACTCACCTCGCCGACCATCCTTTATTGGTGCAACTGA
CCGAGCAAGGTTACTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGCGCTGCCGGCCTGCCGCCGTTCGCCCATCTG
GCCCTGCTGCGCGCCGAAGCGCACAAACCGGGGCAGGCGGAAGCGTTTCTGGATGAGGCGTGCAGCGCGGCGGAACGTTT
GTTGGCCGAGCAGAATCTGAGCGGGATCGAATTGCTTGGCCCCGTGCCGGCGCCGATGGAACGCCGGGCCGGGCGTTATC
GGGCGCAGCTGTTGTTGCAGGCGACGGCGCGGGCGCCACTGCATCGGTTACTCGCCAGCTGGTTGCTGGTGCTGGAGCAG
ATGCCGAGCGGGCGTGCGGTGCGCTGGTCGCTGGATGTCGATCCTGTGGATTTGTATTGA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(207-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A448DKG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.715

100

0.48

Multiple sequence alignment