Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL115_RS08485 Genome accession   NZ_LR134307
Coordinates   1696806..1698224 (-) Length   472 a.a.
NCBI ID   WP_126441784.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC10708     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1691806..1703224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL115_RS08465 (NCTC10708_01678) - 1692591..1693427 (+) 837 WP_126441781.1 putative RNA methyltransferase -
  EL115_RS08470 (NCTC10708_01679) - 1693485..1694165 (-) 681 WP_126441782.1 rhomboid family intramembrane serine protease -
  EL115_RS08475 (NCTC10708_01680) - 1694158..1694685 (-) 528 WP_039677605.1 5-formyltetrahydrofolate cyclo-ligase -
  EL115_RS08480 (NCTC10708_01681) gltX 1695141..1696598 (-) 1458 WP_126441783.1 glutamate--tRNA ligase -
  EL115_RS08485 (NCTC10708_01682) radA 1696806..1698224 (-) 1419 WP_126441784.1 DNA repair protein RadA Machinery gene
  EL115_RS08490 (NCTC10708_01683) - 1698184..1698627 (-) 444 WP_126441785.1 dUTP diphosphatase -
  EL115_RS08495 (NCTC10708_01684) - 1698721..1699755 (-) 1035 WP_126441786.1 S66 family peptidase -
  EL115_RS09655 - 1699974..1700153 (-) 180 Protein_1626 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  EL115_RS08500 (NCTC10708_01685) - 1700238..1701254 (+) 1017 WP_115263535.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  EL115_RS08505 (NCTC10708_01686) galU 1701282..1702181 (+) 900 WP_037598195.1 UTP--glucose-1-phosphate uridylyltransferase GalU -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 51468.27 Da        Isoelectric Point: 6.1053

>NTDB_id=1120918 EL115_RS08485 WP_126441784.1 1696806..1698224(-) (radA) [Streptococcus milleri strain NCTC10708]
MVCGQVVLEALGIKKEESIIAKKKSTFVCQNCKYHSPKYLGRCPNCGAWSSFVEEVEVAEVKNARVSLTGEKSRPMKLAE
VTSINVNRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSHKGTVLYVSGEESAEQIKLRAERLGDID
SEFYLYAETNMQQIRAEIEKIKPDFLIIDSIQTILSPEISSVQGSVSQVREVTAELMQLAKTNNIATFIVGHMTKEGTLA
GPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTR
PILAEVQALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDL
PTNPQECFIGEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLNGLNIPETIEVIGVTTIGEVLKKVFG

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=1120918 EL115_RS08485 WP_126441784.1 1696806..1698224(-) (radA) [Streptococcus milleri strain NCTC10708]
ATGGTGTGCGGACAGGTGGTTTTGGAAGCACTGGGCATTAAGAAAGAGGAATCTATCATAGCCAAGAAAAAATCGACATT
TGTTTGTCAAAATTGTAAATACCATTCTCCCAAGTATCTAGGGCGCTGTCCTAACTGTGGGGCTTGGTCTTCTTTTGTAG
AAGAAGTAGAAGTGGCAGAGGTTAAGAATGCGCGTGTTTCTCTGACAGGTGAAAAAAGTCGACCGATGAAGTTAGCGGAG
GTGACTTCTATCAATGTCAACCGTACCAAGACAGATATGGATGAGTTTAACCGCGTCTTGGGTGGCGGCGTGGTGCCAGG
AAGTTTAGTTCTAATCGGAGGAGATCCCGGTATTGGGAAATCTACCCTTTTATTGCAGGTTTCGACCCAACTATCTCACA
AGGGAACGGTATTGTATGTCAGTGGGGAAGAGTCTGCTGAGCAGATTAAGTTGCGTGCGGAGCGATTAGGCGATATTGAC
AGTGAGTTTTATCTTTATGCCGAGACCAATATGCAGCAGATTCGTGCTGAGATTGAAAAAATTAAGCCAGATTTCTTGAT
TATAGACTCCATTCAGACGATTCTATCGCCAGAAATTTCTAGTGTGCAAGGCTCGGTTTCGCAAGTAAGAGAAGTGACAG
CGGAGTTGATGCAGCTGGCAAAAACCAACAATATTGCCACCTTTATTGTCGGTCATATGACCAAGGAAGGTACCTTGGCT
GGACCTAGAACCTTGGAACACATGGTGGATACGGTACTTTATTTTGAAGGTGAGCGACAGCATACTTTTCGTATTTTGCG
GGCGGTCAAAAACCGCTTCGGCTCTACGAATGAAATCGGCATTTTTGAGATGCAGTCAGGTGGTCTGGTTGAAGTGCTCA
ATCCTAGCCAGGTTTTCTTAGAGGAGCGTTTAGATGGAGCAACAGGCTCTTCTATCGTGGTGACCATGGAGGGGACGCGT
CCAATCCTTGCGGAAGTGCAGGCTTTGGTGACGCCGACTATGTTTGGTAATGCCAAGCGGACAACGACGGGGTTGGACTT
TAATCGCGCCAGTCTGATTATGGCTGTTTTGGAAAAGCGAGCAGGGCTATTATTGCAAAATCAAGATGCCTACCTCAAGT
CAGCAGGCGGTGTCAAATTAGATGAGCCAGCCATTGACTTAGCTGTTGCTGTCGCAATTGCTTCTAGTTATAAAGATTTG
CCAACAAATCCACAAGAATGTTTTATTGGAGAAATCGGTCTGACGGGGGAAATCCGCCGTGTTAATCGCATTGAGCAACG
CATTAATGAAGCTGCTAAGCTAGGCTTCACCAAAGTTTACGCTCCTAAAAACTCTCTGAACGGATTGAACATTCCCGAAA
CTATTGAAGTTATTGGTGTAACAACGATTGGTGAAGTGTTGAAGAAGGTGTTTGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(27-54)

AAA domain (AAA_25)

(84-237)

Subunit ChlI of Mg-chelatase (ChlI)

(380-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

92.035

95.763

0.881
  radA Streptococcus pneumoniae D39

92.035

95.763

0.881
  radA Streptococcus pneumoniae R6

92.035

95.763

0.881
  radA Streptococcus pneumoniae TIGR4

92.035

95.763

0.881
  radA Streptococcus mitis SK321

92.035

95.763

0.881
  radA Streptococcus mitis NCTC 12261

91.814

95.763

0.879
  radA Bacillus subtilis subsp. subtilis str. 168

62.801

96.822

0.608

Multiple sequence alignment