Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   EL115_RS03250 Genome accession   NZ_LR134307
Coordinates   654713..655564 (+) Length   283 a.a.
NCBI ID   WP_003069740.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC10708     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 649713..660564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL115_RS03220 (NCTC10708_00652) - 649935..650813 (+) 879 WP_003069751.1 F0F1 ATP synthase subunit gamma -
  EL115_RS03225 (NCTC10708_00653) atpD 650848..652254 (+) 1407 WP_037597319.1 F0F1 ATP synthase subunit beta -
  EL115_RS03230 (NCTC10708_00654) - 652265..652684 (+) 420 WP_003069748.1 F0F1 ATP synthase subunit epsilon -
  EL115_RS03235 (NCTC10708_00655) - 652788..653018 (+) 231 WP_126441295.1 DUF1146 family protein -
  EL115_RS03240 (NCTC10708_00656) murA 653056..654336 (+) 1281 WP_119055957.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  EL115_RS03245 (NCTC10708_00657) - 654329..654505 (+) 177 WP_003069742.1 DNA-directed RNA polymerase subunit beta -
  EL115_RS03250 (NCTC10708_00658) endA 654713..655564 (+) 852 WP_003069740.1 DNA/RNA non-specific endonuclease Machinery gene
  EL115_RS03255 (NCTC10708_00659) - 655718..657454 (+) 1737 WP_126441296.1 ABC transporter ATP-binding protein -
  EL115_RS03260 (NCTC10708_00660) - 657464..659233 (+) 1770 WP_039676902.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 31034.76 Da        Isoelectric Point: 10.4363

>NTDB_id=1120884 EL115_RS03250 WP_003069740.1 654713..655564(+) (endA) [Streptococcus milleri strain NCTC10708]
MARKKSNQKVAQSVAGVFLALVLALGGYSFSNYHNHSSTKHSDSTAINRSIRKNYAAPSQELAQSVLTESVKRQLKGKIE
WNGAGAFIVNENKNDLDAKVGSIPYADNKTKLVRGRTVPTVANALLSKATRQYRNREETGNGSTTWTPAGWHQVKHLSGE
YHHAVDRGHLLGYALIGNLKGFDASTSNPKNIAVQTAWANQANASHSTGQNFYETKVRKALDKNKRVRYRVTLIYASEQD
LVPVGSHIEAKSSDGSLEINVFVPNVQTGIRLNYQTGEVTVTN

Nucleotide


Download         Length: 852 bp        

>NTDB_id=1120884 EL115_RS03250 WP_003069740.1 654713..655564(+) (endA) [Streptococcus milleri strain NCTC10708]
ATGGCGAGAAAGAAATCCAACCAGAAAGTAGCGCAAAGTGTAGCAGGCGTATTTCTTGCACTTGTTCTTGCGCTGGGAGG
TTATTCTTTTAGCAATTATCATAATCATAGCAGTACGAAGCATTCTGACAGCACTGCTATTAACAGGAGTATTCGTAAGA
ATTATGCAGCACCCAGTCAAGAATTAGCACAGAGCGTTTTAACAGAATCTGTCAAACGACAGCTCAAAGGGAAAATCGAG
TGGAATGGCGCAGGGGCTTTTATTGTCAATGAGAATAAAAATGATTTAGATGCTAAAGTGGGAAGTATTCCTTATGCGGA
TAATAAGACAAAATTGGTCAGAGGTCGAACTGTTCCAACGGTAGCAAATGCTCTTTTGTCCAAAGCTACTCGTCAGTATA
GGAATCGTGAAGAAACAGGTAATGGTTCAACAACTTGGACACCTGCTGGCTGGCATCAGGTGAAGCATTTATCGGGTGAA
TATCATCATGCAGTTGATCGTGGACATCTGCTGGGTTATGCCTTGATTGGTAACTTGAAAGGCTTTGATGCTTCGACTAG
CAATCCCAAAAATATTGCGGTGCAAACGGCTTGGGCGAATCAAGCAAATGCTAGCCATTCTACAGGTCAAAATTTTTATG
AAACGAAAGTACGTAAAGCTCTTGATAAAAATAAACGAGTTCGGTATCGAGTGACCTTGATTTACGCAAGTGAGCAGGAT
TTAGTACCGGTTGGTTCACATATTGAAGCCAAGTCTAGCGACGGTAGTTTGGAAATAAATGTCTTTGTGCCAAACGTACA
GACAGGAATTCGATTAAATTATCAGACAGGAGAAGTGACGGTCACCAACTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(114-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

78.761

79.859

0.629
  endA Streptococcus pneumoniae D39

78.761

79.859

0.629
  endA Streptococcus pneumoniae R6

78.761

79.859

0.629
  endA Streptococcus pneumoniae TIGR4

78.761

79.859

0.629

Multiple sequence alignment