Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL116_RS02545 Genome accession   NZ_LR134304
Coordinates   528184..529548 (+) Length   454 a.a.
NCBI ID   WP_047428255.1    Uniprot ID   A0A2K4AEL2
Organism   Staphylococcus schweitzeri strain NCTC13712     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 523184..534548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL116_RS02525 (NCTC13712_00472) - 523193..523654 (+) 462 WP_000551760.1 CtsR family transcriptional regulator -
  EL116_RS02530 (NCTC13712_00473) - 523673..524239 (+) 567 WP_047449911.1 UvrB/UvrC motif-containing protein -
  EL116_RS02535 (NCTC13712_00474) - 524229..525236 (+) 1008 WP_047428353.1 protein arginine kinase -
  EL116_RS02540 (NCTC13712_00475) - 525250..527706 (+) 2457 WP_047550209.1 ATP-dependent Clp protease ATP-binding subunit -
  EL116_RS02545 (NCTC13712_00476) radA 528184..529548 (+) 1365 WP_047428255.1 DNA repair protein RadA Machinery gene
  EL116_RS02550 (NCTC13712_00477) - 529573..530646 (+) 1074 WP_047550206.1 PIN/TRAM domain-containing protein -
  EL116_RS02555 (NCTC13712_00478) gltX 531134..532588 (+) 1455 WP_047550204.1 glutamate--tRNA ligase -
  EL116_RS02565 (NCTC13712_00480) cysE 533001..533648 (+) 648 WP_072291959.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49820.30 Da        Isoelectric Point: 7.0049

>NTDB_id=1120760 EL116_RS02545 WP_047428255.1 528184..529548(+) (radA) [Staphylococcus schweitzeri strain NCTC13712]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLNQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPKNNIGGWTYPEGIQVIGVTSVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1120760 EL116_RS02545 WP_047428255.1 528184..529548(+) (radA) [Staphylococcus schweitzeri strain NCTC13712]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCCAATTG
TGGTGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCTGCCAATCCAAAGCATGGAGTAAAAACGAAAGAATTAGCTG
GTAAAGTACAAAAATTAAATAGCATTAAACATGAAACGACACCTAGAGTGTTGACAGATTCTGCAGAATTCAATCGTGTA
TTAGGTGGCGGTATTGTTAGCGGATCACTTGTTCTGATAGGTGGAGATCCAGGTATTGGTAAGTCAACATTGCTTTTACA
AATTTGTGCTTCTTTATCACAAAAGAAAAAAGTGTTATATATTACTGGAGAAGAATCTCTTAATCAAACGAAACTTCGAG
CAGAGCGTTTAGATGAAGACTCTAGTGAATTGCAAGTATTAGCAGAAACAGATTTAGAAGTTATATATCAAACAGTTAAA
GAAGAACAACCAGACCTGTTAGTAGTTGATTCTATTCAAACGATTTATCACCCTGAAATTAGTTCGGCACCGGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAACATTGCAAAACAAATGAATATTGCAACTTTTATTGTTGGACATG
TAACGAAAGAAGGTCAAATTGCAGGTCCTAGGTTATTGGAACATATGGTAGATACAGTGCTTTATTTTGAAGGTGATGAA
CATCATGCATATCGAATTTTACGTGCTGTTAAAAATCGTTTTGGTTCTACCAATGAAATGGGTATTTTTGAAATGAAACA
AAGCGGGTTAAAGGGTGTTAATAATCCGTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTACCTGGGTCTACAATTG
TTGCAACAATGGAAGGCACAAGACCACTTTTAATAGAAGTACAAGCGCTTGTAACACCGACAACATTTAATAATCCAAGA
AGAATGGCAACGGGAATTGATCATAATCGACTAAGTTTACTGATGGCTGTTTTAGAAAAGAAAGAAAATTATCTATTGCA
GCAACAAGATGCTTATATCAAAGTCGCAGGTGGAGTGAAATTGACAGAGCCAGCTGTTGATTTAAGTGTAATCGTGGCTA
CCGCATCAAGCTTTAAAGATAAAGCAGTTGATGGTTTAGATTGTTACATTGGAGAAGTTGGTTTAACGGGAGAGGTACGT
CGTGTATCTCGTATAGAACAACGCGTACAAGAAGCTGCAAAGTTAGGCTTCAAACGTGCAATTATTCCTAAAAATAATAT
TGGCGGATGGACATATCCAGAAGGGATTCAAGTAATTGGCGTAACTTCTGTACATGAAGCACTATCATTCGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K4AEL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

61.83

98.678

0.61
  radA Streptococcus pneumoniae D39

61.83

98.678

0.61
  radA Streptococcus pneumoniae R6

61.83

98.678

0.61
  radA Streptococcus pneumoniae TIGR4

61.83

98.678

0.61
  radA Streptococcus mitis NCTC 12261

61.83

98.678

0.61
  radA Streptococcus mitis SK321

61.83

98.678

0.61

Multiple sequence alignment