Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EL258_RS12235 Genome accession   NZ_LR134302
Coordinates   2680420..2681865 (+) Length   481 a.a.
NCBI ID   WP_100853576.1    Uniprot ID   -
Organism   Achromobacter spanius strain NCTC13519     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2675420..2686865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL258_RS12220 (NCTC13519_02457) - 2676208..2677536 (+) 1329 WP_100853579.1 amidase -
  EL258_RS12225 (NCTC13519_02458) leuA 2677711..2679429 (-) 1719 WP_100853578.1 2-isopropylmalate synthase -
  EL258_RS12230 (NCTC13519_02459) - 2679789..2680421 (-) 633 WP_100853577.1 hypothetical protein -
  EL258_RS12235 (NCTC13519_02460) rpoN/rpoN1 2680420..2681865 (+) 1446 WP_100853576.1 RNA polymerase factor sigma-54 Machinery gene
  EL258_RS12240 (NCTC13519_02461) - 2681987..2682268 (+) 282 WP_050448875.1 (2Fe-2S)-binding protein -
  EL258_RS12245 (NCTC13519_02462) bfr 2682772..2683248 (+) 477 WP_050448876.1 bacterioferritin -
  EL258_RS12250 (NCTC13519_02463) - 2683323..2684009 (-) 687 WP_100853575.1 hypothetical protein -
  EL258_RS12255 (NCTC13519_02464) - 2684034..2685641 (-) 1608 WP_100853574.1 peptide chain release factor 3 -
  EL258_RS12260 (NCTC13519_02465) - 2685755..2686384 (-) 630 WP_050448879.1 LysE family translocator -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52465.78 Da        Isoelectric Point: 4.7930

>NTDB_id=1120746 EL258_RS12235 WP_100853576.1 2680420..2681865(+) (rpoN/rpoN1) [Achromobacter spanius strain NCTC13519]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLEREDDTPAAEAQAESERESSVQDDEP
AVERETPVDEMPGSGGVYSDDDSGLPEAARPDTLREHLLGQLMLTRAAPRDAALAALLIDELDENGYLGSPLEEILTWLP
ADMEPDLDELRAALSLLQSFDPPGIGARDMADCLVLQLRHPDLASLPEAADPAVLACARAICAQHLPVLATGNAARLCAA
VGCDEATFRAAHSLILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QTQLQQARWMIRNVEQRFDTILRVAQAIVERQTAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPATLRKAQAGA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=1120746 EL258_RS12235 WP_100853576.1 2680420..2681865(+) (rpoN/rpoN1) [Achromobacter spanius strain NCTC13519]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGACCTGGCCAGCATTTGACGCTGACCCCTCAGCTGCA
GCAATCGATACGATTGTTGCAGTTGTCCACGCTCGACCTTGAGGCCGAGATCTCGCAAGTGCTCGCGGACAATCCCCTGT
TGGAGCGGGAAGACGACACCCCCGCCGCCGAGGCGCAAGCCGAATCCGAGCGCGAATCCTCCGTGCAGGACGATGAGCCC
GCCGTAGAGCGCGAGACGCCCGTGGACGAAATGCCCGGCTCGGGCGGGGTGTATTCGGATGATGACTCCGGCCTGCCCGA
GGCGGCGCGCCCCGACACCCTGCGTGAGCACCTGCTGGGCCAACTGATGTTGACGCGGGCCGCCCCGCGTGACGCGGCGT
TGGCCGCGCTGCTGATCGACGAGCTGGACGAAAACGGGTATCTGGGGTCGCCGCTTGAGGAAATCCTGACCTGGCTGCCC
GCCGACATGGAGCCGGACCTGGACGAATTGCGCGCCGCGCTGTCCTTGTTGCAGTCGTTCGACCCCCCTGGCATCGGCGC
GCGCGACATGGCCGACTGCCTGGTGTTGCAGTTGCGCCACCCTGACCTGGCCAGCCTGCCGGAAGCGGCTGACCCGGCGG
TCCTGGCGTGCGCTCGCGCGATTTGCGCGCAGCATCTGCCCGTGCTGGCCACGGGCAACGCCGCGCGTCTGTGCGCCGCC
GTGGGCTGTGACGAAGCCACCTTTCGCGCCGCCCATTCCCTGATTCTGCGGCTAGAACCCCGCCCCGGGCGGGCCTGGAC
GGTGCCGGCAGCCGATTACGCCGTGCCGGACGTCATCGTGCGCAAGACCCGGCGCGGCTGGCAAGTCACCCTCAATAGCG
CTGCGGTGCCCCGTTTGCAGATCAACGGCCTGTATGCGCAGATGTTGGGCAATCAGAAGGAATCCGCGCACGCGGGCCTG
CAGACCCAATTGCAGCAAGCCAGATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGAGTCGCCCAGGC
GATCGTGGAGCGCCAGACCGCGTTCTTCAGCCAGGGGCCATCCGCCATGCGTCCGCTGATCCTGAAGGATATCGCCGGCG
AACTTGGATTGCACGAATCGACCATTTCACGGGCCACAACACAGAAGTACATGCTCACTCCGTTCGGTACCTTGGAGCTC
AAGCGCTTCTTCGGCACGGGCGTGTCCACGGACGGCGGTGATGCAACTTCCGCCACTGCGGTGCAGACATTCATCCGCCA
AATGGTGGCTGAAGAGAACCGCGCCAAGCCCTTGTCCGATAGCCAGATCATGCAGAAACTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCACTGCGGATTGCCCCCGCCACGCTGCGCAAGGCGCAGGCCGGCGCA
CGTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.713

100

0.511

Multiple sequence alignment