Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL114_RS00815 Genome accession   NZ_LR134297
Coordinates   178390..179760 (+) Length   456 a.a.
NCBI ID   WP_010720638.1    Uniprot ID   A0A1V8X781
Organism   Enterococcus hirae strain NCTC12368     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 173390..184760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL114_RS00790 (NCTC12368_00161) - 174705..175022 (+) 318 WP_010737260.1 hypothetical protein -
  EL114_RS00795 (NCTC12368_00162) - 175111..175455 (-) 345 WP_010720642.1 hypothetical protein -
  EL114_RS00800 (NCTC12368_00163) - 175559..176803 (-) 1245 WP_034700060.1 glutamate-5-semialdehyde dehydrogenase -
  EL114_RS00805 (NCTC12368_00164) proB 176800..177606 (-) 807 WP_010720640.1 glutamate 5-kinase -
  EL114_RS00810 (NCTC12368_00165) - 177808..178338 (+) 531 WP_010720639.1 dUTP diphosphatase -
  EL114_RS00815 (NCTC12368_00166) radA 178390..179760 (+) 1371 WP_010720638.1 DNA repair protein RadA Machinery gene
  EL114_RS00820 (NCTC12368_00167) - 179794..180954 (+) 1161 WP_010720637.1 PIN/TRAM domain-containing protein -
  EL114_RS00825 gltX 181171..182631 (+) 1461 WP_010720636.1 glutamate--tRNA ligase -
  EL114_RS00830 (NCTC12368_00169) epsC 182948..183484 (+) 537 WP_126342868.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49925.56 Da        Isoelectric Point: 7.3886

>NTDB_id=1120647 EL114_RS00815 WP_010720638.1 178390..179760(+) (radA) [Enterococcus hirae strain NCTC12368]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIAEVIPKKEPRVKTKLDELN
RVLGGGVVPGSMILIGGDPGIGKSTLLLQVSQQLADIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLEGATGSAIVVAMEGTRPILVEIQALVTPTMYG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGGWNPPTGIEVVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1120647 EL114_RS00815 WP_010720638.1 178390..179760(+) (radA) [Enterococcus hirae strain NCTC12368]
ATGGCAAAAAAAGCGAAAGTCCAGTTTGTTTGCCAAAATTGTGGTTATGTTTCGCCTAAATTCTTAGGGCGTTGCCCAAA
TTGCGGGAAATGGAACACGATGGTCGAGGAAATCGAGCAAGATACGACTGACCGTAGAACACGAACAAGTTTGACTGGAG
AAAAAGCCAAACCAACGAAAATTGCAGAAGTGATTCCTAAAAAGGAACCACGAGTCAAAACAAAATTAGATGAATTGAAT
CGAGTTTTGGGCGGGGGCGTCGTTCCGGGGTCAATGATTTTGATTGGTGGCGATCCAGGCATCGGTAAATCCACATTACT
TTTACAAGTATCCCAACAATTAGCTGATATCGGCGGGAAAGTATTATATGTTTCTGGAGAAGAAAGTGCAGAACAAATTA
AATTACGTGCCGAGCGTTTGGGCTCAATCAATACAGAATTTTACTTGTATGCTGAAACTGATATGAATGAAATTAGCCGT
GCCATCGAACAGATTTCACCAGATTATGTGATCATTGATTCGATTCAAACGATGACACAACCTGATATTACGAGTGTAGC
AGGGAGTGTCAGTCAAGTGAGAGAAACGACTGCTGAGTTGTTGAAAATAGCGAAAACAAATGGGATTGCGATTTTTATCG
TAGGACATGTAACAAAAGAAGGCTCGATTGCGGGTCCACGTATGCTTGAACATATGGTTGATACTGTTTTGTATTTTGAA
GGAGAACAGCATCATAGTTTTCGTATTTTGAGAGCAGTAAAGAATCGATTTGGTTCAACAAATGAAATTGGTATATTTGA
AATGCATGAACATGGATTAGAAGAAGTTGCGAATCCTTCCCAAATCTTTTTGGAAGAACGATTAGAAGGAGCAACAGGCT
CAGCGATTGTTGTGGCAATGGAAGGTACCCGCCCAATTTTAGTGGAAATCCAAGCACTGGTAACACCAACAATGTATGGA
AATGCTAAACGAACCACTACTGGCTTAGATTTTAACCGAGTATCATTGATCATGGCAGTTCTCGAAAAACGAGCAGGCTT
ACTGTTGCAGAATCAAGATGCTTACTTAAAAGCAGCAGGTGGGGTAAAATTAAATGAACCAGCCATTGATTTAGCGATTG
CTGTGAGTATTGCTTCCAGTTACAAAGAAAAAGGGACACAACCGACAGAATGTTTTATTGGCGAAATCGGGTTGACGGGC
GAAATTCGCCGTGTCAGTCATATTGAACAGCGTGTCAAAGAAGTACAAAAGCTGGGTTTCACAAAAGTTTATTTGCCAAA
GAACAATCTAGGAGGGTGGAATCCTCCGACTGGCATTGAAGTCGTAGGTGTCGCTACCTTGGCGGAAACGTTAAAACGTG
TCTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V8X781

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667

Multiple sequence alignment