Detailed information NTG1120423

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: EL084_RS00950
• Genome accession: NZ_LR134292
• Coordinates: 180551..181483 (+)
• Length: 310 a.a.
• NCBI ID: WP_003067560.1
• Uniprot ID: A0A1G9P2J7
• Organism: Streptococcus equinus strain NCTC10389
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 175551..186483

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL084_RS00930 (NCTC10389_00195)	-	175772..177424 (+)	1653	WP_126439922.1	peptide ABC transporter substrate-binding protein	-
EL084_RS00935 (NCTC10389_00196)	-	177536..178450 (+)	915	WP_003067572.1	ABC transporter permease	-
EL084_RS00940 (NCTC10389_00197)	-	178462..179493 (+)	1032	WP_115254875.1	ABC transporter permease	-
EL084_RS00945 (NCTC10389_00198)	oppD	179505..180551 (+)	1047	WP_024344767.1	ABC transporter ATP-binding protein	Regulator
EL084_RS00950 (NCTC10389_00199)	amiF	180551..181483 (+)	933	WP_003067560.1	ABC transporter ATP-binding protein	Regulator

Sequence

Protein

• Length: 310 a.a.
• Molecular weight: 35035.81 Da
• Isoelectric Point: 5.6591

>NTDB_id=1120423 EL084_RS00950 WP_003067560.1 180551..181483(+) (amiF) [Streptococcus equinus strain NCTC10389]
MTENRKKLVELKNVSLTFNEGKKNEVKAINNVSFDIYEGEVFGLVGESGSGKTTVGRAILKLYDINGGEIDFDGETVSNL
KGKELHEFRKNVQMIFQDPQASLNGRMKIRDIVAEGLDIHKLVNSKEERDEKVQELLSLVGLNKDHMTRYPHEFSGGQRQ
RIGIARALAVQPKFIIADEPISALDVSIQAQVVNLMQKLQRERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSDD
VYNNPIHPYTKSLLSAIPEPDPESERGRVHQVYDPTAELDGQEREMREITPGHFVLSTEAEAEEYKKALQ

Nucleotide

• Length: 933 bp

>NTDB_id=1120423 EL084_RS00950 WP_003067560.1 180551..181483(+) (amiF) [Streptococcus equinus strain NCTC10389]
ATGACTGAAAATAGAAAAAAGCTTGTTGAACTTAAAAATGTATCTTTGACCTTCAATGAAGGTAAAAAGAATGAGGTAAA
AGCAATCAACAACGTTAGCTTTGACATCTACGAAGGCGAAGTATTTGGTCTAGTTGGTGAATCAGGTTCTGGTAAAACAA
CTGTTGGACGTGCTATCTTGAAACTTTATGATATCAATGGTGGAGAAATTGATTTTGATGGTGAAACAGTTTCTAACCTT
AAAGGTAAAGAATTGCACGAATTTCGTAAAAATGTCCAAATGATTTTCCAAGACCCTCAAGCAAGTCTTAATGGTCGTAT
GAAAATTCGTGACATTGTTGCAGAAGGTCTTGATATTCATAAATTAGTTAATAGTAAAGAAGAACGTGATGAAAAAGTTC
AAGAATTACTGTCATTAGTTGGTTTGAACAAAGATCACATGACTCGTTACCCACATGAATTTTCAGGTGGTCAACGTCAA
CGTATCGGTATTGCTCGAGCACTTGCAGTTCAACCCAAATTCATCATTGCTGATGAACCAATTTCAGCCCTTGACGTATC
AATCCAAGCTCAAGTTGTAAACTTGATGCAAAAACTTCAACGTGAACGTGGTTTAACATACTTGTTCATCGCTCACGACT
TGTCTATGGTGAAATACATTTCTGACCGTATCGGTGTTATGCACTGGGGTAAAATGCTTGAAATTGGGACATCTGATGAT
GTTTACAACAATCCAATCCACCCATATACTAAGAGTCTGTTATCTGCTATTCCAGAACCAGATCCAGAATCTGAACGTGG
ACGTGTACACCAAGTGTATGATCCTACTGCAGAGCTTGATGGCCAAGAACGTGAAATGCGAGAAATTACTCCAGGACACT
TTGTACTTTCAACAGAAGCAGAAGCAGAAGAATACAAAAAAGCATTACAATAA

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

• ABC transporter (ABC_tran) (30-181)

3D structure

• AlphaFold DB: A0A1G9P2J7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	56.25	98.065	0.552
amiF	Streptococcus thermophilus LMG 18311	55.921	98.065	0.548
amiF	Streptococcus salivarius strain HSISS4	55.592	98.065	0.545