Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL087_RS00905 Genome accession   NZ_LR134288
Coordinates   164645..166042 (+) Length   465 a.a.
NCBI ID   WP_126408110.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC11169     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 159645..171042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS00875 (NCTC11169_00170) - 159648..160307 (+) 660 WP_126406188.1 hypothetical protein -
  EL087_RS00880 (NCTC11169_00171) dinB 160326..161393 (+) 1068 WP_126406190.1 DNA polymerase IV -
  EL087_RS00885 - 161659..162760 (-) 1102 Protein_136 IS110 family transposase -
  EL087_RS00895 (NCTC11169_00174) - 163093..164127 (+) 1035 WP_126406192.1 S66 family peptidase -
  EL087_RS00900 (NCTC11169_00175) - 164221..164664 (+) 444 WP_126406194.1 dUTP diphosphatase -
  EL087_RS00905 (NCTC11169_00176) radA 164645..166042 (+) 1398 WP_126408110.1 DNA repair protein RadA Machinery gene
  EL087_RS00910 (NCTC11169_00177) gltX 166280..167737 (+) 1458 WP_126406196.1 glutamate--tRNA ligase -
  EL087_RS00915 (NCTC11169_00178) dapD 167851..168549 (+) 699 WP_003039207.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  EL087_RS00920 (NCTC11169_00179) - 168562..169692 (+) 1131 WP_126406199.1 N-acetyldiaminopimelate deacetylase -
  EL087_RS00925 (NCTC11169_00180) - 169737..170264 (+) 528 WP_126406202.1 5-formyltetrahydrofolate cyclo-ligase -
  EL087_RS00930 (NCTC11169_00181) - 170257..170937 (+) 681 WP_003039281.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 50683.21 Da        Isoelectric Point: 5.4188

>NTDB_id=1120229 EL087_RS00905 WP_126408110.1 164645..166042(+) (radA) [Streptococcus milleri strain NCTC11169]
MEVLGIKKEESIIANKKATFVCQNCEYHSPKYLGRCPNCGAWSSFVEEIEVAEVKNARVSLTGEKSRPMKLAEVTSINVN
RTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSHKGTALYVSGEESAEQIKLRAERLGDIDSEFYLYA
ETNMQSIRAEIEKIKPDFLIIDSIQTILSPEISSVQGSVSQVREVTAELMQLAKTNNIATFIVGHMTKEGTLAGPRTLEH
MVDAVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQ
ALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDLPTNPQEC
FIGEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLNGLNIPETIEVIGVTTIGEVLKKVFG

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=1120229 EL087_RS00905 WP_126408110.1 164645..166042(+) (radA) [Streptococcus milleri strain NCTC11169]
TTGGAAGTACTGGGCATTAAGAAAGAGGAATCTATCATAGCCAATAAAAAAGCGACATTTGTTTGTCAAAATTGTGAATA
TCATTCCCCCAAATATCTAGGGCGCTGCCCTAACTGTGGGGCTTGGTCTTCTTTTGTGGAAGAGATCGAAGTTGCAGAGG
TTAAGAATGCGCGTGTTTCTCTGACAGGTGAAAAAAGTCGACCGATGAAGTTAGCGGAGGTGACTTCTATCAATGTCAAC
CGTACCAAGACAGATATGGATGAATTTAATCGCGTCTTGGGCGGCGGTGTGGTGCCAGGAAGTTTAGTTCTAATCGGAGG
AGATCCCGGCATTGGGAAATCTACCCTTTTATTGCAGGTTTCGACCCAACTCTCTCACAAGGGAACAGCATTGTATGTCA
GTGGGGAAGAGTCAGCCGAGCAGATTAAGCTGCGCGCGGAACGTTTAGGCGATATTGACAGTGAATTTTATCTCTATGCG
GAGACCAATATGCAGAGTATTCGTGCTGAGATTGAAAAAATCAAACCAGATTTTTTGATTATAGATTCCATTCAGACGAT
TCTATCGCCAGAAATTTCCAGCGTGCAAGGGTCGGTTTCGCAAGTGCGAGAAGTGACAGCGGAGCTGATGCAGCTGGCAA
AAACCAACAATATTGCCACCTTTATTGTCGGACATATGACCAAGGAAGGTACCTTGGCTGGACCTAGAACTTTGGAACAT
ATGGTGGATGCGGTACTTTATTTTGAGGGTGAGCGGCAGCATACTTTTCGTATTTTGCGGGCGGTCAAAAACCGCTTCGG
CTCCACGAATGAAATCGGTATTTTTGAAATGCAGTCAGGTGGTCTGGTTGAAGTACTCAATCCTAGCCAGGTTTTCTTAG
AGGAGCGTTTAGATGGGGCGACAGGCTCTTCTATCGTGGTGACTATGGAGGGGACGCGTCCAATCCTTGCGGAAGTGCAG
GCTTTGGTGACGCCGACCATGTTTGGCAATGCCAAGCGGACAACGACAGGATTGGATTTCAATCGTGCTAGTCTGATTAT
GGCTGTTTTGGAAAAGCGAGCAGGGTTATTATTGCAAAATCAAGATGCCTACCTCAAGTCAGCAGGCGGTGTCAAATTAG
ATGAGCCAGCTATTGACTTAGCTGTTGCCGTTGCAATTGCTTCGAGTTATAAAGATTTGCCAACGAATCCACAAGAATGT
TTTATTGGTGAAATCGGTCTGACGGGTGAAATCCGCCGTGTCAATCGCATTGAGCAGCGCATCAATGAAGCTGCTAAGCT
GGGCTTCACCAAAGTTTACGCTCCTAAAAACTCTCTGAACGGATTGAACATTCCCGAAACTATTGAAGTTATTGGTGTAA
CAACGATTGGTGAAGTATTGAAGAAGGTGTTTGGGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(20-47)

Subunit ChlI of Mg-chelatase (ChlI)

(373-437)

AAA domain (AAA_25)

(77-230)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

91.593

97.204

0.89
  radA Streptococcus pneumoniae D39

91.593

97.204

0.89
  radA Streptococcus pneumoniae R6

91.593

97.204

0.89
  radA Streptococcus pneumoniae TIGR4

91.593

97.204

0.89
  radA Streptococcus mitis SK321

91.593

97.204

0.89
  radA Streptococcus mitis NCTC 12261

91.372

97.204

0.888
  radA Bacillus subtilis subsp. subtilis str. 168

61.926

98.28

0.609

Multiple sequence alignment