Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EL070_RS03750 Genome accession   NZ_LR134284
Coordinates   713375..715180 (+) Length   601 a.a.
NCBI ID   WP_030127119.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8232     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 695691..732955 713375..715180 within 0


Gene organization within MGE regions


Location: 695691..732955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL070_RS03665 (NCTC8232_00723) - 696220..697839 (+) 1620 WP_111705681.1 DEAD/DEAH box helicase -
  EL070_RS03670 (NCTC8232_00724) - 697966..699966 (+) 2001 WP_111705680.1 potassium transporter Kup -
  EL070_RS03675 (NCTC8232_00725) - 699990..700268 (-) 279 WP_003061674.1 GIY-YIG nuclease family protein -
  EL070_RS03680 (NCTC8232_00726) - 700258..701034 (-) 777 WP_111705883.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  EL070_RS03685 (NCTC8232_00727) - 701152..701892 (+) 741 WP_002989170.1 lysophospholipid acyltransferase family protein -
  EL070_RS03690 (NCTC8232_00728) - 702092..702754 (+) 663 WP_002983967.1 ComEA family DNA-binding protein -
  EL070_RS03695 (NCTC8232_00729) - 702735..704978 (+) 2244 WP_111705679.1 DNA internalization-related competence protein ComEC/Rec2 -
  EL070_RS03700 (NCTC8232_00730) holA 705049..706089 (+) 1041 WP_011017949.1 DNA polymerase III subunit delta -
  EL070_RS03705 (NCTC8232_00731) sodA 706186..706791 (+) 606 WP_011017948.1 superoxide dismutase SodA -
  EL070_RS03710 (NCTC8232_00732) - 706958..707104 (+) 147 WP_011017947.1 hypothetical protein -
  EL070_RS03715 (NCTC8232_00733) - 707160..707408 (+) 249 WP_030127124.1 serine hydrolase -
  EL070_RS03720 (NCTC8232_00734) - 707405..707995 (+) 591 WP_111705678.1 serine hydrolase -
  EL070_RS03725 (NCTC8232_00735) - 708109..709329 (-) 1221 WP_111705677.1 DUF3114 domain-containing protein -
  EL070_RS03730 (NCTC8232_00736) queA 709336..710364 (-) 1029 WP_111705676.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  EL070_RS03735 (NCTC8232_00737) nagB 710566..711270 (+) 705 WP_111705675.1 glucosamine-6-phosphate deaminase -
  EL070_RS03740 (NCTC8232_00738) - 711389..712105 (+) 717 WP_011528761.1 pseudouridine synthase -
  EL070_RS03745 (NCTC8232_00739) - 712400..713362 (+) 963 WP_002983995.1 competence protein CoiA -
  EL070_RS03750 (NCTC8232_00740) pepF 713375..715180 (+) 1806 WP_030127119.1 oligoendopeptidase F Regulator
  EL070_RS03755 (NCTC8232_00741) - 715554..716750 (+) 1197 WP_126405130.1 OFA family MFS transporter -
  EL070_RS03760 (NCTC8232_00742) - 716816..717523 (+) 708 WP_111705673.1 O-methyltransferase -
  EL070_RS03765 (NCTC8232_00743) prsA 717586..718641 (+) 1056 WP_111705672.1 peptidylprolyl isomerase PrsA -
  EL070_RS03770 (NCTC8232_00745) alaS 719028..721646 (+) 2619 WP_126405131.1 alanine--tRNA ligase -
  EL070_RS03775 (NCTC8232_00746) - 722006..722221 (+) 216 WP_030127117.1 helix-turn-helix transcriptional regulator -
  EL070_RS03780 (NCTC8232_00747) - 722218..722979 (+) 762 WP_053308456.1 DUF3169 family protein -
  EL070_RS03785 (NCTC8232_00748) - 722998..723693 (+) 696 WP_111705671.1 CPBP family intramembrane glutamic endopeptidase -
  EL070_RS03790 - 723790..724940 (-) 1151 Protein_668 IS3 family transposase -
  EL070_RS03795 (NCTC8232_00753) - 725036..726262 (-) 1227 WP_415245014.1 chloride channel protein -
  EL070_RS03800 (NCTC8232_00754) nrdF 726324..727283 (-) 960 WP_111705669.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  EL070_RS03805 (NCTC8232_00755) nrdE 727616..729775 (-) 2160 WP_111705668.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  EL070_RS03810 (NCTC8232_00756) nrdH 729795..730013 (-) 219 WP_111705667.1 glutaredoxin-like protein NrdH -
  EL070_RS03815 (NCTC8232_00757) - 730407..730670 (+) 264 WP_002984031.1 phosphocarrier protein HPr -
  EL070_RS03820 (NCTC8232_00758) ptsP 730675..732408 (+) 1734 WP_111705666.1 phosphoenolpyruvate--protein phosphotransferase -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69408.48 Da        Isoelectric Point: 4.8408

>NTDB_id=1120105 EL070_RS03750 WP_030127119.1 713375..715180(+) (pepF) [Streptococcus pyogenes strain NCTC8232]
MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFAGHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKND
QDTTVAKYQEYQAKASGLYAKFSEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
QEIFNGAEETFSILDNADIVFPIVRNDKGENIELTHGNFISLMESKDRSVRQAAYEAMYSTYEQFQHTYAKTLQTNVKVQ
NYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNKHLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALE
KAEKVLAVFGKDYADRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSLHSTFTRET
QPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFRGTVFRQTQFAEFEHAIHQADQKGEVLTSEY
LNQLYADLNEKYYGLSKKDNHFIQYEWARIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPL
EVIAKAGVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=1120105 EL070_RS03750 WP_030127119.1 713375..715180(+) (pepF) [Streptococcus pyogenes strain NCTC8232]
ATGACTGATAATCGCAGCCATTTAGAAGAAAAATACACTTGGGATTTGAGCACTATTTTTGCAACAGATAAGGATTGGGA
AGCTGAAGTCAGTGACTTAGCTACTGAAGTTGAGGCATCTAAAGGTTTTGCTGGTCATTTGCTGGATTCTAGTGCTAATT
TGTTAAAGGTTACAAAAACATATTTAGAATTGGCTCGTCGTGTGGAGAAAGTCTATGTTTATGCTCACATGAAAAATGAC
CAAGACACTACTGTTGCCAAGTATCAAGAATACCAAGCTAAGGCTTCAGGCTTGTATGCAAAGTTTAGCGAAGTCTTTTC
ATTCTATGATCCAGAAGTGATGATGCTTCATCAAGAAGACTATCAGGCTTTCCTTACTGAAACCCCTGAGTTGAAAGTGT
ACAACCATTTCTTTGACAAATTATTCCAAGCCCGCGAACATGTTCTTAGTCAAGCAGAAGAGGAATTATTAGCAGGAGCT
CAAGAAATTTTCAATGGTGCGGAAGAAACCTTTAGTATTCTTGATAACGCTGACATTGTTTTCCCGATAGTCAGAAATGA
TAAAGGTGAAAACATTGAATTAACCCATGGTAACTTCATTAGCCTAATGGAATCTAAGGACCGTAGCGTTCGCCAAGCTG
CTTATGAAGCCATGTATAGTACTTATGAACAGTTCCAACATACTTACGCTAAAACCTTGCAAACCAATGTGAAAGTGCAA
AACTATAAGGCGCGTGTTCATAAATACGATTCAGCTCGTCAGGCTGCCATGGCTGCTAACTTTATTCCTGAAGCTGTTTA
CGATACCTTGTTAGAAACGGTTAATAAACATTTGCCACTCTTACACCGTTATTTGAAATTGCGTCAAGAGGTTCTTGGTT
TAGACGATTTGAAGATGTATGATGTTTACACACCACTATCTGAAACAGATCTAGCCATTGGTTATGATGAGGCTTTGGAA
AAGGCTGAGAAGGTTTTGGCTGTTTTTGGTAAGGATTATGCAGACCGAGTGCACCGTGCCTTTACAGAACGTTGGATTGA
TGTTCATGTCAATAAAGGGAAGCGCTCTGGAGCTTATTCGGGTGGTTCCTATGATACCAATGCCTTCATGCTGTTGAACT
GGCAAGATACACTTGATAATCTTTACACACTTGTCCACGAAACAGGCCACAGTTTGCATTCTACTTTCACTCGTGAAACA
CAGCCTTATGTGTATGGGGATTATAGTATCTTCTTGGCAGAAATTGCATCCACAACGAATGAAAATATTATGACAGAAGC
TCTCTTAAATGAGGTTCAAGATGAGAAAGAACGCTTTGCCATTTTAAACCATTACCTCGATGGTTTCCGTGGAACAGTTT
TCCGTCAAACCCAATTTGCTGAGTTTGAACATGCCATTCATCAAGCTGATCAAAAAGGAGAAGTGTTAACAAGTGAATAC
CTTAACCAGCTATACGCTGATTTAAATGAAAAATATTATGGTTTAAGTAAAAAAGATAATCATTTTATTCAGTATGAATG
GGCACGTATTCCTCATTTCTATTACAATTATTATGTTTACCAATATGCAACAGGGTTTGCAGCTGCAAGCTATTTAGCGG
ATAAGATTGTGCATGGCACACAAGATGATATCGATCATTACTTGGCCTACCTTAAGTCAGGAAATTCTGATTACCCACTT
GAAGTGATTGCTAAAGCAGGCGTTGATATGGCAAAAGGTGATTATTTGGAAGCCGCCTTTAAAGTCTTTGACGAACGTCT
AACAGAACTTGAAGTACTCGTGTCCAAAGGCATACACAAAAGCTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-181)

Peptidase family M3 (Peptidase_M3)

(202-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

70.549

100

0.705

Multiple sequence alignment