Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOA_RS10180 Genome accession   NZ_CP004006
Coordinates   2094109..2095227 (-) Length   372 a.a.
NCBI ID   WP_025386255.1    Uniprot ID   W0BGJ9
Organism   Legionella oakridgensis ATCC 33761 = DSM 21215     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2089109..2100227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOA_RS10165 (Loa_02266) - 2089825..2090973 (+) 1149 WP_025386252.1 patatin-like phospholipase family protein -
  LOA_RS10170 (Loa_02267) - 2090989..2091726 (+) 738 WP_025386253.1 acetoacetate decarboxylase -
  LOA_RS10175 (Loa_02268) ankK 2091851..2093788 (-) 1938 WP_025386254.1 Dot/Icm T4SS effector AnkK/LegA5 -
  LOA_RS10180 (Loa_02269) pilU 2094109..2095227 (-) 1119 WP_025386255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOA_RS10185 (Loa_02270) - 2095484..2096065 (+) 582 WP_025386256.1 hypothetical protein -
  LOA_RS10190 (Loa_02271) - 2096069..2098606 (+) 2538 WP_025386257.1 PD-(D/E)XK nuclease family protein -
  LOA_RS15900 (Loa_02272) - 2098682..2098969 (+) 288 WP_274544555.1 NAD-dependent epimerase/dehydratase family protein -
  LOA_RS15905 (Loa_02273) - 2098966..2099601 (+) 636 WP_274544556.1 epimerase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41651.98 Da        Isoelectric Point: 5.6474

>NTDB_id=112007 LOA_RS10180 WP_025386255.1 2094109..2095227(-) (pilU) [Legionella oakridgensis ATCC 33761 = DSM 21215]
MDIIPFLKLMVDRGASDLFFSVGTPPHIKIEGVTSPIGQAPLKSQQMAEIAKSMMNDQQRKEFDATMELNMAISLDNIGR
YRINLFRQRGEIAMVVRYIKGMIPSLADLNLPPILSSIVMEQRGLILVVGSTGSGKSTTLASMIDYRNSHECGHILTIED
PVEFIHLHKKSVVDQREVGIDTLNYDNALKNAMREAPDVILIGEIRDRNTMKHAISYAETGHLCLSTLHANNANQTMDRI
INFFPEEAKQQLLLDLSLNLRAIVSLRLIPGLHDKRVPAVEVLLNTPYIADLIDKGKIDEIKEVMAKSTDQGMQTFDQAL
FDLYKAGKISQENAIRYADSKNNVGLKIRLSEEGNFDKDTDLTIEQGDLDKF

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=112007 LOA_RS10180 WP_025386255.1 2094109..2095227(-) (pilU) [Legionella oakridgensis ATCC 33761 = DSM 21215]
ATGGATATTATTCCTTTTTTAAAATTAATGGTTGACCGTGGCGCATCGGATTTATTTTTTAGCGTTGGCACGCCTCCGCA
CATTAAAATCGAAGGCGTTACTTCGCCAATAGGACAAGCGCCGCTTAAATCTCAACAAATGGCAGAAATCGCCAAATCCA
TGATGAATGATCAACAACGAAAAGAATTTGATGCCACGATGGAATTAAATATGGCTATTTCACTGGACAATATTGGCCGA
TATCGCATCAATTTATTTCGGCAACGTGGTGAAATTGCAATGGTGGTACGTTACATTAAAGGCATGATTCCTTCCTTGGC
AGATTTAAACTTGCCTCCGATTTTAAGTTCCATCGTTATGGAACAGCGCGGTCTTATTCTGGTAGTCGGTTCAACGGGAT
CAGGTAAATCAACAACGTTGGCCTCTATGATTGATTATCGTAACAGTCATGAATGTGGCCATATTTTAACCATTGAAGAT
CCCGTTGAATTCATCCATCTGCATAAAAAATCGGTGGTTGATCAGCGTGAAGTTGGCATAGATACATTAAACTATGATAA
TGCATTGAAAAATGCCATGCGGGAAGCACCGGATGTCATTCTGATAGGTGAGATTCGTGACCGTAACACCATGAAACATG
CGATATCTTATGCCGAAACAGGTCATTTATGCTTATCCACCTTGCATGCTAATAATGCCAATCAAACCATGGATCGCATT
ATTAATTTTTTTCCAGAAGAAGCAAAACAACAGTTGTTGCTGGATTTATCTTTGAATTTACGTGCCATTGTTTCCTTGCG
TTTAATTCCCGGCCTGCATGACAAACGAGTTCCTGCTGTGGAAGTGTTGTTGAACACACCGTATATCGCGGATTTGATTG
ATAAGGGAAAAATTGATGAAATTAAGGAAGTGATGGCAAAAAGTACTGATCAGGGTATGCAAACTTTTGATCAGGCATTA
TTTGATTTATACAAGGCAGGTAAGATCAGTCAGGAAAATGCAATTCGTTATGCCGATTCCAAAAACAATGTCGGCTTAAA
GATTCGCTTGTCAGAAGAAGGTAATTTTGATAAGGACACGGACTTAACCATCGAGCAAGGGGACTTGGATAAATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0BGJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.733

100

0.522
  pilU Acinetobacter baylyi ADP1

52.793

96.237

0.508
  pilU Vibrio cholerae strain A1552

48.286

94.086

0.454
  pilT Legionella pneumophila strain Lp02

43.275

91.935

0.398
  pilT Legionella pneumophila strain ERS1305867

43.275

91.935

0.398
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

91.129

0.395
  pilT Neisseria meningitidis 8013

40.805

93.548

0.382
  pilT Neisseria gonorrhoeae MS11

40.805

93.548

0.382
  pilT Pseudomonas aeruginosa PAK

40.816

92.204

0.376
  pilT Acinetobacter baylyi ADP1

40.588

91.398

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.233

92.204

0.371
  pilT Vibrio cholerae strain A1552

41.641

88.441

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.641

88.441

0.368
  pilT Acinetobacter baumannii strain A118

40.12

89.785

0.36
  pilT Acinetobacter nosocomialis M2

40.12

89.785

0.36
  pilT Acinetobacter baumannii D1279779

40.12

89.785

0.36

Multiple sequence alignment