Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL077_RS01245 Genome accession   NZ_LR134273
Coordinates   235462..236823 (+) Length   453 a.a.
NCBI ID   WP_012514884.1    Uniprot ID   B4U0R5
Organism   Streptococcus equi subsp. equi strain NCTC12091     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 230462..241823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL077_RS01230 (NCTC12091_00248) - 232044..233840 (+) 1797 WP_126436277.1 ABC transporter ATP-binding protein -
  EL077_RS01235 (NCTC12091_00249) - 234002..234769 (+) 768 WP_012678598.1 epoxyqueuosine reductase QueH -
  EL077_RS01240 (NCTC12091_00250) - 234949..235395 (+) 447 WP_126436279.1 dUTP diphosphatase -
  EL077_RS01245 (NCTC12091_00251) radA 235462..236823 (+) 1362 WP_012514884.1 DNA repair protein RadA Machinery gene
  EL077_RS01250 (NCTC12091_00252) - 237644..238150 (+) 507 WP_014622237.1 beta-class carbonic anhydrase -
  EL077_RS01255 (NCTC12091_00253) - 238278..238991 (+) 714 WP_037580456.1 TIGR00266 family protein -
  EL077_RS01260 (NCTC12091_00254) gltX 239599..241044 (+) 1446 WP_126436281.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49333.77 Da        Isoelectric Point: 6.7545

>NTDB_id=1119741 EL077_RS01245 WP_012514884.1 235462..236823(+) (radA) [Streptococcus equi subsp. equi strain NCTC12091]
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIE
AIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1119741 EL077_RS01245 WP_012514884.1 235462..236823(+) (radA) [Streptococcus equi subsp. equi strain NCTC12091]
ATAGCTAAGAAAAAGGCGAGGTTTATCTGTGGAGAATGTGGCTATCAGTCACCCAAATATCTGGGGCGCTGCCCTAATTG
CTCTGCCTGGTCTTCCTTTGTAGAGGAGACAGAGGTCAAGGACGTCAAAAATGCCCGTATTAGCCTGACTGGAGAAAAAA
GTAAGCCCATCAAGCTAAAGCATGTCAATGGCGTTCGCTACCAGCGTATCCAGACAGAGATGAATGAGTTCAATCGTGTG
CTGGGCGGTGGTGTTGTGCCGGGGAGCTTGGTTTTGATTGGTGGTGATCCTGGTATTGGTAAGTCAACGCTACTCTTGCA
AGTTTCTATCCAGCTTGCGGATAAGGGAACTGTTCTATATGTCTCTGGAGAGGAATCGGCAGAGCAGATCAAGATGCGCA
GTGATCGCTTGGGACAGACCGACAATGACTTTTACCTCTATGCGGAGACCAATATGCAGGCTATTCGGGCAGAGATTGAA
GCCATTCAGCCGGATTTTTTGATTATTGATTCTATTCAGACGATCATGAGTCCGGAGATTTCGGGTGTTCAGGGCTCGGT
CAGTCAGGTGAGAGAGGTTACTGCTGAGCTACTCCAGCTAGCTAAGTCTAATCATATTGCAACCTTTATCGTAGGACATG
TCACCAAGGAGGGTACCCTAGCAGGGCCACGTATGCTGGAGCATATGGTTGATACTGTGCTTTATTTTGAAGGGGAAAGA
CACCACACCTTCCGAATTCTGCGAGCTGTTAAAAACCGTTTTGGCTCAACCAATGAGATTGGTATTTTTGACATGCAATC
AGGTGGCTTAGTGGAGGTTCTCAATCCCAGTCAGGTTTTCTTAGAGGAGCGATTAGATGGGGCAACAGGCTCTGCTGTGG
TTGTCACCATGGAGGGGAGCAGACCGATTTTGGCAGAGGTGCAGGCCTTAGTCACACCTACTGTTTTTGGTAATGCTAGA
CGAACGACGACTGGGTTGGACTTTAATCGCGTCAGTCTGATTATGGCTGTTTTGGAAAAGCGCTGCGGGCTTTTATTACA
AAATCAAGATGCCTACCTCAAATCAGCAGGAGGTGTGAAGCTAGATGAGCCTGCTATTGATTTGGCAGTTGCTGTGGCTA
TTGCTTCTAGCTACAAGGAAAAACCAACCAACCCTCAGGAGGCCTTCTTAGGAGAGATTGGCTTGACGGGCGAGATTCGG
CGGGTCACTCGTATTGAGCAGCGGATCAATGAGGCTGCAAAGCTTGGCTTTACCAAGCTTTACGCTCCTAAAAGCTCATT
GCAGGGCATTGACATACCAAGTGGGATTGAGGTTGTTGGTGTAATGACAGTAGGAGAGGTCTTAAAGGCAGTCTTTGCTT
AG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4U0R5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

84.327

100

0.843
  radA Streptococcus pneumoniae D39

84.327

100

0.843
  radA Streptococcus pneumoniae R6

84.327

100

0.843
  radA Streptococcus pneumoniae TIGR4

84.327

100

0.843
  radA Streptococcus mitis SK321

84.327

100

0.843
  radA Streptococcus mitis NCTC 12261

84.106

100

0.841
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment