Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL085_RS10960 Genome accession   NZ_LR134267
Coordinates   2328281..2329651 (-) Length   456 a.a.
NCBI ID   WP_014614769.1    Uniprot ID   A0A166QVY3
Organism   Staphylococcus pseudintermedius strain NCTC5661     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2323281..2334651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL085_RS10935 (SAMEA3504576_02246) cysE 2323446..2324111 (-) 666 WP_172426181.1 serine O-acetyltransferase -
  EL085_RS10940 (SAMEA3504576_02248) gltX 2324499..2325953 (-) 1455 WP_037543943.1 glutamate--tRNA ligase -
  EL085_RS10945 (SAMEA3504576_02249) ispF 2326030..2326509 (-) 480 WP_014614766.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EL085_RS10950 (SAMEA3504576_02250) ispD 2326502..2327200 (-) 699 WP_099986823.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EL085_RS10955 (SAMEA3504576_02251) - 2327201..2328262 (-) 1062 WP_014614768.1 PIN/TRAM domain-containing protein -
  EL085_RS10960 (SAMEA3504576_02252) radA 2328281..2329651 (-) 1371 WP_014614769.1 DNA repair protein RadA Machinery gene
  EL085_RS10965 (SAMEA3504576_02253) - 2330038..2330784 (-) 747 WP_015728605.1 ABC transporter permease -
  EL085_RS10970 (SAMEA3504576_02254) - 2330771..2331475 (-) 705 WP_014614771.1 ABC transporter ATP-binding protein -
  EL085_RS10975 (SAMEA3504576_02255) - 2331490..2332449 (-) 960 WP_243134261.1 hypothetical protein -
  EL085_RS10980 (SAMEA3504576_02256) - 2332449..2333261 (-) 813 WP_063278650.1 SagB family peptide dehydrogenase -
  EL085_RS10985 (SAMEA3504576_02257) - 2333281..2334501 (-) 1221 WP_100007028.1 YcaO-like family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49994.48 Da        Isoelectric Point: 7.0051

>NTDB_id=1119625 EL085_RS10960 WP_014614769.1 2328281..2329651(-) (radA) [Staphylococcus pseudintermedius strain NCTC5661]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLDDIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIVIATASSFKDQAVDGLDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWHFPEGIEVIGVTSVNEALKYALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1119625 EL085_RS10960 WP_014614769.1 2328281..2329651(-) (radA) [Staphylococcus pseudintermedius strain NCTC5661]
GTGGCAAAAACAAAAACAATTTTTGAATGTACGGCCTGTGGTTATCAATCGCCTAAATGGATGGGGAAATGTCCAAATTG
TGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTACGGGCGCAAAAAACAA
GTTCAGCAAAAATACAAAAGCTCGATGACATTAAACAAGAGCTTGCGCCACGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGAATAGGGAAGTCCACATTGTT
ACTTCAAATGTGCGCGGCTTTATCTAAAGAGAAAAGGGTGCTCTATATTACTGGTGAGGAATCACTCAATCAAACTAAAA
TTAGAGCGGATCGTTTGGATGAAGATGCGAGTCAACTTAACGTGTTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAGGTCGAACCTGATTTAATTGTAGTGGACTCTATTCAGACGATCTACCATCCCGAAATCAGTTCGGCGCCTGG
CTCTGTATCTCAAGTTCGTGAAAGTACACAAAGCTTGATGCACATCGCAAAACAAATGAATGTTGCGACGTTTATTGTAG
GCCATGTGACTAAAGAAGGCCAAATCGCGGGGCCGCGTTTACTTGAACATATGGTGGATACGGTGCTTTATTTTGAAGGT
GATGAACATCATGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGTATTTTTGAAAT
GAAACAGTCAGGTTTAAAAAGCGTGCTTAACCCGTCAGAAATGTTTTTAGAAGAACGAACGACCAATGTTGCAGGTTCGA
CAATTGTCGCAACGATGGAAGGGACACGACCATTGTTAATTGAAGTACAAGCGCTTGTCACACCGACGACGTTCAATAAT
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTGTTAATGGCGGTGTTAGAGAAAAAAGAAGGTTATTT
ATTACAACAACAAGACGCTTACATTAAGGTGGCTGGGGGCGTCAAACTCTCTGAACCTGCTGTCGACTTAGGTATCGTCA
TTGCGACAGCTTCTAGTTTTAAAGACCAAGCAGTAGACGGCCTCGATTGTTATATTGGCGAGGTTGGATTGACTGGTGAG
GTCCGCCGTGTTTCGCGCATTGAGCAACGTGTGCAAGAAGCAGCAAAATTGGGCTTTAAACGTGTGATTATCCCTAAAAA
CAATATTGGCGGATGGCACTTCCCAGAAGGTATTGAAGTGATTGGCGTGACTTCTGTCAATGAAGCATTGAAATACGCAT
TAAAAAATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A166QVY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.658
  radA Streptococcus pneumoniae Rx1

58.628

99.123

0.581
  radA Streptococcus pneumoniae D39

58.628

99.123

0.581
  radA Streptococcus pneumoniae R6

58.628

99.123

0.581
  radA Streptococcus pneumoniae TIGR4

58.628

99.123

0.581
  radA Streptococcus mitis NCTC 12261

58.628

99.123

0.581
  radA Streptococcus mitis SK321

58.628

99.123

0.581

Multiple sequence alignment