Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EL075_RS10790 Genome accession   NZ_LR134244
Coordinates   2222205..2223569 (-) Length   454 a.a.
NCBI ID   WP_002465557.1    Uniprot ID   A0A364UNL0
Organism   Staphylococcus warneri strain NCTC7291     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2217205..2228569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL075_RS10775 (NCTC7291_02220) cysE 2218341..2218970 (-) 630 WP_171001928.1 serine O-acetyltransferase -
  EL075_RS10780 (NCTC7291_02222) gltX 2219365..2220819 (-) 1455 WP_126403334.1 glutamate--tRNA ligase -
  EL075_RS10785 (NCTC7291_02223) - 2221105..2222178 (-) 1074 WP_126403335.1 PIN/TRAM domain-containing protein -
  EL075_RS10790 (NCTC7291_02224) radA 2222205..2223569 (-) 1365 WP_002465557.1 DNA repair protein RadA Machinery gene
  EL075_RS10795 (NCTC7291_02225) - 2223911..2226364 (-) 2454 WP_126403336.1 ATP-dependent Clp protease ATP-binding subunit -
  EL075_RS10800 (NCTC7291_02226) - 2226380..2227384 (-) 1005 WP_002465569.1 protein arginine kinase -
  EL075_RS10805 (NCTC7291_02227) - 2227377..2227934 (-) 558 WP_049415263.1 UvrB/UvrC motif-containing protein -
  EL075_RS10810 (NCTC7291_02228) - 2227955..2228416 (-) 462 WP_002451197.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49837.32 Da        Isoelectric Point: 6.8989

>NTDB_id=1119381 EL075_RS10790 WP_002465557.1 2222205..2223569(-) (radA) [Staphylococcus warneri strain NCTC7291]
MAKKKIIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKSKETAGKVQTLDSIKNESTPRVLTESKEFNRV
LGGGIVNGSLVLIGGDPGIGKSTLLLQICASLSQSKNVLYITGEESINQTKLRADRLEEDSSKLQVLAETDLEVIYQTVK
DINPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVSTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDKAVDGLDCFIGEVGLTGEVR
RVARIEQRVQEAAKLGFKRVIIPQTNIGGWTFPEDIKVVGVSSVHEALKYAFNS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=1119381 EL075_RS10790 WP_002465557.1 2222205..2223569(-) (radA) [Staphylococcus warneri strain NCTC7291]
TTGGCCAAGAAAAAGATAATATTTGAATGTATGGCATGTGGCTATCAATCTCCAAAATGGATGGGAAAGTGTCCTAATTG
TGGTGCATGGAACCAGATGGAAGAAATAGTAGAAAAAGCTGCCAATCCTAAACATGGTGTTAAATCTAAAGAAACAGCAG
GGAAAGTACAAACATTAGATAGTATCAAAAATGAATCTACACCACGCGTTCTTACAGAATCTAAAGAATTTAACCGTGTA
CTAGGGGGAGGAATAGTTAATGGATCATTAGTATTAATAGGCGGAGATCCAGGTATTGGTAAATCCACGTTACTACTACA
AATTTGTGCCTCACTCTCACAATCTAAAAATGTTCTATATATCACAGGTGAGGAATCAATCAATCAAACAAAATTACGTG
CAGATCGTTTGGAAGAAGACTCGAGTAAATTACAAGTATTAGCTGAAACGGATTTAGAAGTCATTTATCAAACAGTTAAA
GACATTAATCCGGATTTACTAGTAGTTGACTCTATTCAAACAATATACCATCCAGAAATTAGCTCTGCACCAGGATCTGT
GTCACAGGTTAGAGAAAGTACGCAAAGCTTAATGAATATAGCTAAACAAATGAACATAGCTACATTTATTGTTGGGCATG
TTACAAAAGAAGGACAAATTGCAGGACCTAGATTACTTGAACACATGGTGGATACTGTATTGTATTTTGAAGGTGATGAG
CATCATGCTTATCGAATACTACGAGCAGTTAAAAACCGTTTTGGTTCGACTAATGAGATGGGAATATTTGAAATGAAACA
AAGTGGTCTAAAAGGTGTGCTAAATCCTTCAGAGATGTTTTTAGAAGAACGTTCAACTAATGTGCCAGGTTCAACAATTG
TTTCAACTATGGAAGGTACAAGACCACTACTTATTGAAGTACAGGCGCTTGTAACACCGACAACATTTAATAATCCACGT
CGTATGGCTACCGGTATCGATCACAATCGTTTAAGCTTATTAATGGCTGTTTTAGAGAAAAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATTAAGGTTGCTGGCGGCGTGAAATTAACTGAACCTGCAGTTGATTTAAGTATCATTGTAGCTA
CAGCTTCAAGCTTTAAAGACAAAGCTGTAGACGGCTTAGACTGCTTTATTGGCGAGGTTGGTCTAACAGGAGAAGTAAGA
CGTGTAGCTAGAATAGAACAACGTGTTCAAGAAGCAGCAAAGTTAGGATTTAAGAGAGTTATTATTCCTCAAACTAATAT
AGGTGGTTGGACTTTCCCGGAAGATATAAAAGTAGTAGGTGTTTCATCAGTACATGAAGCATTAAAATACGCATTTAATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(69-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A364UNL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.105

100

0.674
  radA Streptococcus pneumoniae Rx1

60.398

99.559

0.601
  radA Streptococcus pneumoniae D39

60.398

99.559

0.601
  radA Streptococcus pneumoniae R6

60.398

99.559

0.601
  radA Streptococcus pneumoniae TIGR4

60.398

99.559

0.601
  radA Streptococcus mitis NCTC 12261

60.398

99.559

0.601
  radA Streptococcus mitis SK321

60.398

99.559

0.601

Multiple sequence alignment