Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EL050_RS14005 Genome accession   NZ_LR134165
Coordinates   2782742..2783512 (-) Length   256 a.a.
NCBI ID   WP_020452240.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain NCTC8721     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2777742..2788512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL050_RS13985 (NCTC8721_02868) - 2777817..2778629 (-) 813 WP_023855239.1 pyruvate, water dikinase regulatory protein -
  EL050_RS13990 (NCTC8721_02869) - 2778655..2779293 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  EL050_RS13995 (NCTC8721_02870) glyS 2779491..2781530 (-) 2040 WP_025811143.1 glycine--tRNA ligase subunit beta -
  EL050_RS14000 (NCTC8721_02871) glyQ 2781523..2782410 (-) 888 WP_020452239.1 glycine--tRNA ligase subunit alpha -
  EL050_RS14005 (NCTC8721_02873) recO 2782742..2783512 (-) 771 WP_020452240.1 DNA repair protein RecO Machinery gene
  EL050_RS14010 (NCTC8721_02874) - 2783550..2783693 (-) 144 WP_003183626.1 YqzL family protein -
  EL050_RS14015 (NCTC8721_02875) era 2783855..2784760 (-) 906 WP_023855242.1 GTPase Era -
  EL050_RS14020 (NCTC8721_02876) - 2784741..2785151 (-) 411 WP_020452242.1 cytidine deaminase -
  EL050_RS14025 (NCTC8721_02877) - 2785284..2785664 (-) 381 WP_020452243.1 diacylglycerol kinase family protein -
  EL050_RS14030 (NCTC8721_02878) ybeY 2785642..2786118 (-) 477 WP_025811142.1 rRNA maturation RNase YbeY -
  EL050_RS14035 (NCTC8721_02879) - 2786115..2788169 (-) 2055 WP_228119640.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29527.17 Da        Isoelectric Point: 8.3124

>NTDB_id=1118605 EL050_RS14005 WP_020452240.1 2782742..2783512(-) (recO) [Bacillus paralicheniformis strain NCTC8721]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRH
IREDLFLTAYAAYITELIDKGTEEKKPNPFLFELLLQSMRRLDDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=1118605 EL050_RS14005 WP_020452240.1 2782742..2783512(-) (recO) [Bacillus paralicheniformis strain NCTC8721]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAAATCATTACGCTATTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCTTCTTTTTTATTCCGCAAATCATCCGGTCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGCAT
ATACGCGAGGATCTGTTTTTAACAGCATACGCGGCCTACATCACTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTTCTTCAGTCGATGAGGAGGCTCGATGACGGTACAGATCCCGACATTATCGCCAACC
TTATCGAAGTTAAAATGCTCAGCGTGATGGGACTGTATCCGGAGCTGGATGAATGTGTACATTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGGCTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCGGTTAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTCATACAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGGCGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

77.344

100

0.773

Multiple sequence alignment