Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BLEG1_RS00035 Genome accession   NZ_CP003923
Coordinates   5897..7270 (+) Length   457 a.a.
NCBI ID   WP_038475738.1    Uniprot ID   A0A060LR47
Organism   Shouchella lehensis G1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 897..12270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLEG1_RS00015 (BleG1_0003) - 1209..1679 (+) 471 WP_038475726.1 CtsR family transcriptional regulator -
  BLEG1_RS00020 (BleG1_0004) - 1696..2238 (+) 543 WP_038475729.1 UvrB/UvrC motif-containing protein -
  BLEG1_RS00025 (BleG1_0005) - 2235..3305 (+) 1071 WP_038475732.1 protein arginine kinase -
  BLEG1_RS00030 (BleG1_0006) clpC 3336..5789 (+) 2454 WP_038475735.1 ATP-dependent protease ATP-binding subunit ClpC -
  BLEG1_RS00035 (BleG1_0007) radA 5897..7270 (+) 1374 WP_038475738.1 DNA repair protein RadA Machinery gene
  BLEG1_RS00040 (BleG1_0008) disA 7276..8349 (+) 1074 WP_038475741.1 DNA integrity scanning diadenylate cyclase DisA -
  BLEG1_RS00045 (BleG1_0009) - 8504..9595 (+) 1092 WP_038475744.1 PIN/TRAM domain-containing protein -
  BLEG1_RS00050 (BleG1_0010) ispD 9693..10406 (+) 714 WP_038475747.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BLEG1_RS00055 (BleG1_0011) ispF 10381..10857 (+) 477 WP_038475751.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49826.55 Da        Isoelectric Point: 6.7570

>NTDB_id=111837 BLEG1_RS00035 WP_038475738.1 5897..7270(+) (radA) [Shouchella lehensis G1]
MAKVKTKFVCQECGYESAKWMGKCPGCQSWNTLVEEKAVQEKQRSRSYVTSGTTVHKAQPITKVVGEKEPRVSTTMPELN
RVLGGGIVPGSLVLVGGDPGIGKSTLLLQLSAKLANTKERVLYISGEESVKQTKLRADRLELTSEDLYVLAETDVSLIEQ
AIDEVQPSLVIIDSIQTVYQEDITSAPGSVAQVRECTATFMRIAKTRGTAVFIVGHVTKQGSIAGPKLLEHMVDSVLYFE
GERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPMGFG
NPRRMATGLDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIALSIASSFRNQPTEPMDVAIGEVGLTG
EIRRVSRIEQRVNEAFKLGFKRVIIPEKNLGGWTVPDGIEVVGVPTLDKALDVALGR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=111837 BLEG1_RS00035 WP_038475738.1 5897..7270(+) (radA) [Shouchella lehensis G1]
ATGGCAAAAGTAAAAACAAAGTTTGTCTGTCAAGAATGTGGCTATGAGTCAGCGAAGTGGATGGGGAAGTGTCCAGGGTG
TCAAAGTTGGAATACCCTCGTTGAAGAAAAAGCCGTTCAAGAGAAACAGCGTTCAAGAAGTTATGTAACATCTGGAACGA
CTGTGCATAAAGCACAGCCTATTACTAAAGTGGTAGGGGAAAAAGAGCCTCGGGTTAGCACAACGATGCCAGAATTAAAC
CGAGTGCTTGGTGGAGGCATCGTTCCGGGTTCACTTGTTTTAGTAGGTGGCGACCCGGGTATTGGAAAGTCAACGCTACT
ATTGCAACTATCTGCGAAATTAGCAAATACGAAAGAACGAGTTCTTTATATTTCAGGAGAAGAGTCGGTTAAACAAACGA
AGCTAAGAGCAGATCGCTTAGAACTAACTTCTGAAGATTTATACGTACTCGCTGAGACAGATGTGTCACTCATTGAACAA
GCTATTGATGAAGTGCAACCTTCGCTAGTTATTATTGATTCGATTCAAACCGTGTATCAAGAGGATATAACCTCTGCTCC
AGGAAGTGTTGCGCAAGTGCGCGAATGCACAGCAACGTTTATGCGCATTGCTAAAACAAGAGGCACCGCAGTCTTTATTG
TTGGGCATGTGACAAAACAAGGATCCATCGCAGGTCCGAAGCTGCTTGAGCATATGGTAGACTCGGTCTTGTATTTTGAA
GGGGAACGACACCACACGTACCGTATTTTAAGAGCGGTGAAAAACCGATTTGGCTCCACAAATGAAATGGGTATTTTCGA
AATGAAAGAGCTTGGTTTAGAGGAAGTCTTGAACCCATCAGAAATCTTTCTTGAGGAACGGTCACAAGGAGCAGCTGGCT
CGGTTGTAGTGGCATCGATGGAAGGAACCCGACCTGTTCTTGTTGAATTACAAGCATTAATCTCCCCGATGGGCTTTGGG
AATCCAAGAAGGATGGCAACAGGCTTAGATCACAACCGGGTATCTCTCATAATGGCTGTTCTTGAAAAGCGGGTAGGGAT
GCTTTTACAAAACCAAGACGCCTATTTAAAGGTTGCCGGTGGTGTGAAGCTAGATGAGCCAGCCATTGATCTTGCTATTG
CTTTAAGTATCGCATCAAGTTTTCGTAATCAGCCTACAGAACCGATGGATGTAGCCATTGGTGAGGTGGGACTGACCGGT
GAGATTAGACGAGTATCGAGAATTGAACAACGAGTTAATGAAGCATTTAAACTAGGTTTTAAACGAGTTATTATACCGGA
AAAGAATTTAGGTGGTTGGACCGTACCAGATGGCATTGAGGTCGTAGGTGTTCCAACTCTTGATAAAGCGCTCGATGTTG
CATTAGGGAGGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A060LR47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.085

100

0.731
  radA Streptococcus pneumoniae Rx1

60.31

98.687

0.595
  radA Streptococcus pneumoniae D39

60.31

98.687

0.595
  radA Streptococcus pneumoniae R6

60.31

98.687

0.595
  radA Streptococcus pneumoniae TIGR4

60.31

98.687

0.595
  radA Streptococcus mitis NCTC 12261

60.31

98.687

0.595
  radA Streptococcus mitis SK321

60.31

98.687

0.595

Multiple sequence alignment