Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EW025_RS09275 Genome accession   NZ_LR130238
Coordinates   1768425..1769786 (-) Length   453 a.a.
NCBI ID   WP_010921866.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain HKU419 isolate HKU419     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1763425..1774786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW025_RS09255 - 1763530..1764876 (-) 1347 WP_011285380.1 sensor histidine kinase -
  EW025_RS09260 gltX 1765271..1766716 (-) 1446 WP_002986105.1 glutamate--tRNA ligase -
  EW025_RS09265 - 1766898..1767608 (-) 711 WP_011017311.1 TIGR00266 family protein -
  EW025_RS09270 - 1767739..1768236 (-) 498 WP_010921867.1 beta-class carbonic anhydrase -
  EW025_RS09275 radA 1768425..1769786 (-) 1362 WP_010921866.1 DNA repair protein RadA Machinery gene
  EW025_RS09280 - 1769867..1770313 (-) 447 WP_002986111.1 dUTP diphosphatase -
  EW025_RS09285 - 1770423..1771190 (-) 768 WP_002986113.1 epoxyqueuosine reductase QueH -
  EW025_RS09290 - 1771308..1773092 (-) 1785 WP_010921865.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49372.61 Da        Isoelectric Point: 5.8179

>NTDB_id=1116641 EW025_RS09275 WP_010921866.1 1768425..1769786(-) (radA) [Streptococcus pyogenes strain HKU419 isolate HKU419]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLNAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1116641 EW025_RS09275 WP_010921866.1 1768425..1769786(-) (radA) [Streptococcus pyogenes strain HKU419 isolate HKU419]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCCACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGTGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACATATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTGCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATTTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGCTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGCTTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTCGGAGTGACTACGGTTGGTCAGGTTTTAAATGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.504

99.779

0.614

Multiple sequence alignment