Detailed information    

insolico Bioinformatically predicted

Overview

Name   sepM   Type   Regulator
Locus tag   EW024_RS06395 Genome accession   NZ_LR130237
Coordinates   1240782..1241819 (-) Length   345 a.a.
NCBI ID   WP_002983824.1    Uniprot ID   A0A4U9C0Q7
Organism   Streptococcus pyogenes strain SP444 isolate SP444     
Function   processing of CSP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1235782..1246819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW024_RS06375 - 1236531..1237158 (+) 628 Protein_1179 prepilin peptidase -
  EW024_RS06380 rlmN 1237240..1238319 (-) 1080 WP_002992788.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EW024_RS06385 - 1238323..1238943 (-) 621 WP_002992791.1 YutD family protein -
  EW024_RS06390 - 1239391..1240368 (-) 978 WP_002994809.1 LacI family DNA-binding transcriptional regulator -
  EW024_RS06395 sepM 1240782..1241819 (-) 1038 WP_002983824.1 SepM family pheromone-processing serine protease Regulator
  EW024_RS06400 coaD 1241806..1242297 (-) 492 WP_002983821.1 pantetheine-phosphate adenylyltransferase -
  EW024_RS06405 rsmD 1242287..1242826 (-) 540 WP_002983819.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EW024_RS06410 asnA 1242949..1243941 (-) 993 WP_002983817.1 aspartate--ammonia ligase -
  EW024_RS06415 arcC 1244254..1245204 (-) 951 WP_011528820.1 carbamate kinase -
  EW024_RS06420 - 1245224..1246555 (-) 1332 WP_011054719.1 dipeptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37555.27 Da        Isoelectric Point: 9.6692

>NTDB_id=1116575 EW024_RS06395 WP_002983824.1 1240782..1241819(-) (sepM) [Streptococcus pyogenes strain SP444 isolate SP444]
MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTVLQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTP
FTEISTAEDTTGGYSDADFLRINQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
QFTSSAELIDYVSHLKLGDEVTIQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSVNSEDTVIFSTKGVGGPSAGLMF
TLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGGAGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRA
AKRLKTKMKIVPVTTVQEALVYLRK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1116575 EW024_RS06395 WP_002983824.1 1240782..1241819(-) (sepM) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGAAAAGACTTAAAAAAATCAAATGGTGGTTAGTGGGTCTGCTAGCTTTAATCTCTTTGTTGCTAGCGTTATTTTTTCC
GCTACCTTATTATATTGAAATGCCTGGAGGCGCTTACGATATTCGGACTGTCTTACAAGTCAATGGCAAAGAAGACAAAC
GAAAAGGAGCTTACCAGTTTGTTGCAGTGGGCATTAGTCGTGCCAGCCTCGCTCAGCTATTATATGCTTGGCTGACACCG
TTTACTGAAATTAGTACAGCAGAAGATACAACAGGCGGATACAGCGATGCTGATTTCCTTCGAATTAATCAATTTTACAT
GGAAACATCACAAAATGCAGCTATTTATCAAGCTTTATCCTTAGCTGGAAAACCAGTTACATTAGATTATAAAGGCGTAT
ATGTTTTAGACGTAAACAACGAATCTACTTTTAAAGGAACGTTACACTTAGCAGATACTGTAACAGGTGTAAATGGTAAA
CAGTTTACTAGTTCAGCAGAACTTATTGACTATGTTTCTCACCTAAAACTAGGGGATGAAGTTACGATTCAGTTTACGAG
TGATAATAAGCCTAAAAAAGGAGTTGGCCGTATTATCAAACTGAAAAATGGGAAAAATGGGATTGGCATTGCCTTGACTG
ATCATACAAGTGTCAATTCAGAAGACACAGTGATCTTTAGTACTAAAGGAGTAGGAGGACCTAGTGCTGGTCTAATGTTT
ACTCTTGATATATATGATCAAATAACTAAAGAAGATTTACGCAAGGGCCGTACAATTGCAGGTACAGGAACTATTGGCAA
GGATGGCGAAGTAGGAGATATTGGTGGTGCAGGTCTTAAAGTAGTTGCAGCAGCTGAAGCTGGTGCAGATATATTTTTTG
TTCCGAATAATCCTGTTGATAAGGAAATTAAAAAAGTTAATCCAAATGCTATAAGTAATTACGAAGAAGCCAAACGGGCA
GCCAAACGACTAAAGACCAAAATGAAGATTGTTCCTGTTACGACTGTTCAAGAGGCACTGGTTTATCTTCGCAAATAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(233-309)

PDZ domain (PDZ_2)

(129-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U9C0Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sepM Streptococcus mutans UA159

68.986

100

0.69

Multiple sequence alignment