Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EW024_RS05835 Genome accession   NZ_LR130237
Coordinates   1139785..1141590 (-) Length   601 a.a.
NCBI ID   WP_002995768.1    Uniprot ID   A0A660A6L4
Organism   Streptococcus pyogenes strain SP444 isolate SP444     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1116522..1149404 1139785..1141590 within 0


Gene organization within MGE regions


Location: 1116522..1149404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW024_RS05745 - 1116523..1117980 (-) 1458 Protein_1055 IS1182 family transposase -
  EW024_RS05750 - 1118147..1119421 (-) 1275 WP_011528746.1 polysaccharide deacetylase family protein -
  EW024_RS05755 - 1119515..1120942 (-) 1428 WP_002989248.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  EW024_RS05760 ptsP 1121127..1122860 (-) 1734 WP_011528747.1 phosphoenolpyruvate--protein phosphotransferase -
  EW024_RS05765 - 1122865..1123128 (-) 264 WP_002984031.1 phosphocarrier protein HPr -
  EW024_RS05770 - 1123521..1123739 (+) 219 WP_042769861.1 redoxin NrdH -
  EW024_RS05775 nrdE 1123759..1125918 (+) 2160 WP_011528750.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  EW024_RS05780 nrdF 1126122..1127081 (+) 960 WP_011017933.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  EW024_RS05785 - 1127078..1128553 (-) 1476 Protein_1063 IS1182 family transposase -
  EW024_RS05790 - 1128701..1129927 (+) 1227 WP_021299359.1 chloride channel protein -
  EW024_RS05795 - 1130023..1131173 (+) 1151 Protein_1065 IS3 family transposase -
  EW024_RS05800 - 1131270..1131965 (-) 696 WP_011528757.1 CPBP family intramembrane glutamic endopeptidase -
  EW024_RS05805 - 1131984..1132745 (-) 762 WP_011528758.1 DUF3169 family protein -
  EW024_RS05810 - 1132742..1132957 (-) 216 WP_002989220.1 helix-turn-helix transcriptional regulator -
  EW024_RS05815 alaS 1133317..1135935 (-) 2619 WP_011528759.1 alanine--tRNA ligase -
  EW024_RS05820 prsA 1136322..1137377 (-) 1056 WP_002984003.1 peptidylprolyl isomerase PrsA -
  EW024_RS05825 - 1137440..1138147 (-) 708 WP_011528760.1 O-methyltransferase -
  EW024_RS05830 - 1138213..1139409 (-) 1197 WP_002995765.1 OFA family MFS transporter -
  EW024_RS05835 pepF 1139785..1141590 (-) 1806 WP_002995768.1 oligoendopeptidase F Regulator
  EW024_RS05840 - 1141603..1142565 (-) 963 WP_002983995.1 competence protein CoiA -
  EW024_RS05845 - 1142860..1143576 (-) 717 WP_011528761.1 pseudouridine synthase -
  EW024_RS05850 nagB 1143695..1144399 (-) 705 WP_021299319.1 glucosamine-6-phosphate deaminase -
  EW024_RS05855 queA 1144601..1145629 (+) 1029 WP_011528763.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  EW024_RS05860 - 1145636..1146856 (+) 1221 WP_011528764.1 DUF3114 domain-containing protein -
  EW024_RS05865 - 1146970..1147805 (-) 836 Protein_1079 serine hydrolase -
  EW024_RS05875 - 1147861..1148007 (-) 147 WP_011528767.1 hypothetical protein -
  EW024_RS05880 sodA 1148174..1148779 (-) 606 WP_002983977.1 superoxide dismutase SodA -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69394.45 Da        Isoelectric Point: 4.8408

>NTDB_id=1116571 EW024_RS05835 WP_002995768.1 1139785..1141590(-) (pepF) [Streptococcus pyogenes strain SP444 isolate SP444]
MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFAGHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKND
QDTTVAKYQEYQAKASGLYAKFSEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
QEIFNGAEETFSILDNADIVFPIVRNDKGENVELTHGNFISLMESKDRSVRQAAYEAMYSTYEQFQHTYAKTLQTNVKVQ
NYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNKHLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALE
KAEKVLAVFGKDYADRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSLHSTFTRET
QPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFRGTVFRQTQFAEFEHAIHQADQKGEVLTSEY
LNQLYADLNEKYYGLSKKDNHFIQYEWARIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPL
EVIAKAGVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=1116571 EW024_RS05835 WP_002995768.1 1139785..1141590(-) (pepF) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGACTGATAATCGCAGCCATTTAGAAGAAAAATACACTTGGGATTTGAGCACTATTTTTGCAACAGATAAGGATTGGGA
AGCTGAAGTCAGTGACTTAGCTACTGAAGTTGAGGCATCTAAAGGTTTTGCTGGTCATTTGCTGGATTCTAGTGCTAATT
TGTTAAAGGTTACAAAAACATATTTAGAATTGGCTCGTCGTGTGGAGAAAGTCTATGTTTATGCTCACATGAAAAATGAC
CAAGACACTACTGTTGCCAAGTATCAAGAATACCAAGCTAAGGCTTCAGGCTTGTATGCAAAGTTTAGCGAAGTCTTTTC
ATTCTATGATCCAGAAGTGATGATGCTTCATCAAGAAGACTATCAGGCTTTCCTTACTGAAACCCCTGAGTTGAAAGTGT
ACAACCATTTCTTTGACAAATTATTCCAAGCCCGCGAACATGTTCTTAGTCAAGCAGAAGAGGAATTATTAGCAGGAGCT
CAAGAAATTTTCAATGGTGCGGAAGAAACCTTTAGTATTCTTGATAACGCTGACATTGTTTTCCCGATAGTCAGAAATGA
TAAAGGTGAAAACGTTGAATTAACCCATGGTAACTTCATTAGCCTAATGGAATCTAAGGACCGTAGCGTTCGCCAAGCTG
CTTATGAAGCCATGTATAGTACTTATGAACAGTTCCAACATACTTACGCTAAAACCTTGCAAACCAATGTGAAAGTGCAA
AACTATAAGGCGCGTGTTCATAAATACGATTCAGCACGTCAAGCTGCCATGGCTGCTAACTTTATTCCAGAAGCTGTTTA
CGACACTTTATTAGAAACGGTTAACAAACATTTACCACTCTTACACCGTTATTTGAAATTGCGTCAAGAGGTTCTTGGTT
TAGACGATTTGAAGATGTATGATGTTTACACGCCATTATCTGAAACAGATTTAGCCATTGGTTATGATGAGGCTTTGGAA
AAGGCTGAGAAGGTTTTGGCTGTTTTTGGTAAGGATTACGCAGATCGTGTACATCGTGCTTTTACCGAACGTTGGATTGA
TGTTCACGTCAATAAAGGGAAGCGCTCTGGGGCTTATTCAGGTGGTTCCTATGATACCAATGCCTTCATGCTGTTGAACT
GGCAAGATACACTTGATAATCTTTACACACTTGTCCACGAAACAGGCCACAGTTTGCATTCTACTTTCACTCGTGAAACA
CAGCCTTATGTGTATGGGGATTATAGTATCTTCTTGGCAGAAATTGCATCCACAACGAATGAAAATATTATGACAGAAGC
TCTCTTAAATGAGGTTCAAGATGAGAAAGAACGCTTTGCCATTTTAAACCATTACCTCGATGGTTTCCGTGGAACAGTTT
TCCGTCAAACCCAATTTGCTGAGTTTGAACATGCCATTCATCAAGCTGATCAAAAAGGAGAAGTGTTAACAAGTGAATAC
CTTAACCAGCTATACGCTGATTTAAATGAAAAATATTATGGTTTAAGTAAAAAAGATAATCATTTTATTCAGTATGAATG
GGCACGTATTCCTCATTTCTATTACAATTATTATGTTTACCAATATGCAACAGGGTTTGCAGCTGCAAGCTATTTAGCGG
ATAAGATTGTGCATGGCACACAAGATGATATCGATCATTACTTGGCCTACCTTAAGTCAGGAAATTCTGATTACCCACTT
GAAGTGATTGCTAAAGCAGGCGTTGATATGGCAAAAGGTGATTATTTGGAAGCCGCCTTTAAAGTCTTTGACGAACGTCT
AACAGAACTTGAAGTACTCGTGTCCAAAGGCATACACAAAAGCTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-181)

Peptidase family M3 (Peptidase_M3)

(202-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A660A6L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

70.715

100

0.707

Multiple sequence alignment