Detailed information    

insolico Bioinformatically predicted

Overview

Name   sepM   Type   Regulator
Locus tag   EW021_RS06465 Genome accession   NZ_LR130236
Coordinates   1237736..1238773 (-) Length   345 a.a.
NCBI ID   WP_010922499.1    Uniprot ID   A0A4U9I5V9
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   processing of CSP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1232736..1243773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS06445 - 1233487..1234113 (+) 627 WP_010922497.1 prepilin peptidase -
  EW021_RS06450 rlmN 1234195..1235274 (-) 1080 WP_002992788.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  EW021_RS06455 - 1235278..1235898 (-) 621 WP_011285637.1 YutD family protein -
  EW021_RS06460 - 1236346..1237322 (-) 977 Protein_1212 LacI family DNA-binding transcriptional regulator -
  EW021_RS06465 sepM 1237736..1238773 (-) 1038 WP_010922499.1 SepM family pheromone-processing serine protease Regulator
  EW021_RS06470 coaD 1238760..1239251 (-) 492 WP_002983821.1 pantetheine-phosphate adenylyltransferase -
  EW021_RS06475 rsmD 1239241..1239780 (-) 540 WP_002983819.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  EW021_RS06480 asnA 1239903..1240895 (-) 993 WP_010922500.1 aspartate--ammonia ligase -
  EW021_RS06485 arcC 1241208..1242158 (-) 951 WP_010922501.1 carbamate kinase -
  EW021_RS06490 - 1242178..1243509 (-) 1332 WP_011285641.1 dipeptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37541.24 Da        Isoelectric Point: 9.6692

>NTDB_id=1116513 EW021_RS06465 WP_010922499.1 1237736..1238773(-) (sepM) [Streptococcus pyogenes strain 5448 isolate 5448]
MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTVLQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTP
FTEISTAEDTTGGYSDADFLRINQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSVNSEDTVIFSTKGVGGPSAGLMF
TLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGGAGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRA
AKRLKTKMKIVPVTTVQEALVYLRK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1116513 EW021_RS06465 WP_010922499.1 1237736..1238773(-) (sepM) [Streptococcus pyogenes strain 5448 isolate 5448]
ATGAAAAGACTTAAAAAAATCAAATGGTGGTTAGTGGGTCTGCTAGCTTTAATCTCTTTGTTGCTAGCGTTATTTTTTCC
GCTACCTTATTATATTGAAATGCCTGGAGGCGCTTACGATATTCGGACTGTCTTACAAGTCAATGGCAAAGAAGACAAAC
GAAAAGGAGCTTACCAGTTTGTTGCAGTGGGCATTAGTCGTGCCAGCCTCGCTCAGCTATTATATGCTTGGCTGACACCG
TTTACTGAAATTAGTACAGCAGAAGATACAACAGGCGGATACAGCGATGCTGATTTCCTTCGAATTAATCAATTTTACAT
GGAAACATCACAAAATGCAGCTATTTATCAAGCTTTATCCTTAGCTGGAAAACCAGTTACATTAGATTATAAAGGCGTAT
ATGTTTTAGACGTAAACAACGAATCTACTTTTAAAGGAACGCTACACTTAGCAGATACTGTAACAGGTGTAAATGGTAAA
CAGTTTACTAGTTCAGCAGAACTTATTGACTATGTTTCTCACCTAAAACTAGGGGATGAAGTTACGGTTCAGTTTACGAG
TGATAATAAGCCTAAAAAAGGAGTTGGCCGTATTATCAAACTGAAAAATGGGAAAAATGGGATTGGCATTGCCTTGACTG
ATCATACAAGTGTCAATTCAGAAGACACAGTGATCTTTAGTACTAAAGGAGTAGGAGGACCTAGTGCTGGTCTAATGTTT
ACTCTTGATATATATGATCAAATAACTAAAGAAGATTTACGCAAGGGCCGTACAATTGCAGGTACAGGAACTATTGGCAA
GGATGGCGAAGTAGGAGATATTGGTGGTGCAGGTCTTAAAGTAGTTGCAGCAGCTGAAGCTGGTGCAGATATATTTTTTG
TTCCGAATAATCCTGTTGATAAGGAAATTAAAAAAGTTAATCCAAATGCTATAAGTAATTACGAAGAAGCCAAACGGGCA
GCCAAACGACTAAAGACCAAAATGAAGATTGTTCCTGTTACGACTGTTCAAGAGGCACTGGTTTATCTTCGCAAATAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(233-309)

PDZ domain (PDZ_2)

(129-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U9I5V9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sepM Streptococcus mutans UA159

69.275

100

0.693

Multiple sequence alignment