Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LBH_RS01575 Genome accession   NZ_CP002427
Coordinates   304147..305523 (+) Length   458 a.a.
NCBI ID   WP_025283283.1    Uniprot ID   -
Organism   Lactobacillus helveticus H9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 299147..310523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LBH_RS01555 (LBH_0289) pepC 300091..301440 (-) 1350 WP_003625856.1 aminopeptidase C -
  LBH_RS01560 (LBH_0290) - 301751..303010 (-) 1260 WP_025283281.1 RNA-guided endonuclease TnpB family protein -
  LBH_RS01565 (LBH_0291) - 303159..303461 (-) 303 WP_025283282.1 hypothetical protein -
  LBH_RS01570 (LBH_0292) - 303596..304147 (+) 552 WP_003625858.1 dUTP diphosphatase -
  LBH_RS01575 (LBH_0293) radA 304147..305523 (+) 1377 WP_025283283.1 DNA repair protein RadA Machinery gene
  LBH_RS01580 (LBH_0294) gltX 305599..307098 (+) 1500 WP_025283284.1 glutamate--tRNA ligase -
  LBH_RS01585 (LBH_0295) cysS 307191..308621 (+) 1431 WP_025283285.1 cysteine--tRNA ligase -
  LBH_RS01590 (LBH_0296) - 308614..309057 (+) 444 WP_003625863.1 Mini-ribonuclease 3 -
  LBH_RS01595 (LBH_0297) rlmB 309044..309796 (+) 753 WP_003625865.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LBH_RS01600 (LBH_0298) - 309939..310484 (+) 546 WP_025283286.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50309.84 Da        Isoelectric Point: 6.5937

>NTDB_id=111631 LBH_RS01575 WP_025283283.1 304147..305523(+) (radA) [Lactobacillus helveticus H9]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVHLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLHIMSDLAEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHTYRILHSVKNRFGSANEIGMFEMKNEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDIYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRIFIPKHNMYQSLTENGIEVIPVASIPQALELVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=111631 LBH_RS01575 WP_025283283.1 304147..305523(+) (radA) [Lactobacillus helveticus H9]
ATGGCTAGAGTAAAAACGCAATATAAATGTCGTTCCTGTGGTTATATTTCAGCTAGTTACTTGGGTCGTTGTCCTAACTG
CGGTGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAGAAAAGATCTACTAAAGCAACGGCAAGCCGTTTAATTC
AAAAAACAGGTCTTAATGAACCTGTTCATTTGGATAAAATCAAGGCTGAAAAAGAAGAACGAATTCCTACTAAGTCCGAA
GAATTAAATCGTGTATTAGGTGGCGGGATCGTTCCTGGATCCCTCGTCTTAATTGGTGGGGACCCTGGTATTGGTAAGTC
AACTTTAATGTTGCATATTATGAGTGATCTGGCAGAAAAATATAAAGTGCTTTATGTTTCAGGAGAAGAGTCAGCTAATC
AAATCAAGCTGAGGGCAGATCGGCTTGGAGTTGGTCAAAGCAACATGCTTTTATATCCAGAAACAGATATGCATGATATT
CGTGAGCAGATCAATGATGTTAAGCCTGATTTTGTCGTTATTGATTCTATTCAAACGATGAATGAACCTAGTCTTGATTC
AATGACTGGTTCTGCTTCACAAGTACGTGAGGTAACAAGTGAATTGATGAAGATTGCTAAGATGGATGCAATTACTGTTT
TTGTTATTGGACACGTGACTAAAGAAGGTGCGATTGCTGGCCCAAAAATTTTGGAACACATGGTGGATACTGTCCTTTAT
TTTGAGGGGGATGAACACCACACGTACCGGATTTTGCATTCCGTAAAAAATCGTTTTGGTTCTGCTAACGAAATCGGCAT
GTTTGAAATGAAAAATGAAGGCTTACGTGAAGTAACCAATCCATCTTCGATTTTCCTTGATCAAAGATTACCTAATTCAA
CTGGGTCAGCCGTAGTAGTTTCACTTGAAGGAACGCGGCCACTTTTGGCAGAAATTCAAGCATTAGTAACGCCAACTGCA
TTTGGCTATGCCAAACGAACTACTTCAGGAATTAGTTTTAATAAAGCTTCACTATTATTAGCGGTTCTTGAAAAACGCGG
CAATTTAATGCTTCAAAATCAGGATATTTATTTAACGGCTACTGGCGGTATTAAATTAAATGAACCTGCAATCGATCTAG
CTATTGCTATGTCAGTAGCATCAAGCTACACCGATAAAGAAATCTCACCGACTGATTGTTTTGTAGGGGAAGTGGGATTA
ACCGGTGAAGTTCGCCGCGTTGATAAAATCGAAGCTAGAGTAAAGGAAGCTGCCAAGGTAGGTTTTAAACGTATTTTTAT
TCCTAAGCATAATATGTATCAAAGTCTAACGGAAAATGGCATTGAAGTAATTCCAGTTGCCAGCATTCCACAGGCATTAG
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

AAA domain (AAA_25)

(66-223)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.33

99.782

0.572
  radA Streptococcus pneumoniae D39

57.33

99.782

0.572
  radA Streptococcus pneumoniae R6

57.33

99.782

0.572
  radA Streptococcus pneumoniae TIGR4

57.33

99.782

0.572
  radA Streptococcus mitis NCTC 12261

57.33

99.782

0.572
  radA Streptococcus mitis SK321

57.33

99.782

0.572
  radA Bacillus subtilis subsp. subtilis str. 168

56.209

100

0.563

Multiple sequence alignment