Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E7X43_RS01460 Genome accession   NZ_LR031358
Coordinates   294647..296059 (+) Length   470 a.a.
NCBI ID   WP_071448846.1    Uniprot ID   A0A6N4A8T2
Organism   Oenococcus oeni strain CRBO_1381     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 289647..301059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7X43_RS01420 (OENI_0299) - 290155..290823 (+) 669 WP_032817273.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  E7X43_RS01425 (OENI_0300) - 290998..291276 (+) 279 WP_002817904.1 hypothetical protein -
  E7X43_RS01430 (OENI_0301) - 291282..291887 (+) 606 WP_002818135.1 DNA-3-methyladenine glycosylase I -
  E7X43_RS01435 (OENI_0302) - 291884..292933 (-) 1050 WP_032817275.1 AI-2E family transporter -
  E7X43_RS01440 (OENI_0303) - 293024..293317 (-) 294 WP_002822408.1 GNAT family N-acetyltransferase -
  E7X43_RS01445 (OENI_0304) - 293421..293612 (+) 192 WP_002818138.1 hypothetical protein -
  E7X43_RS01450 (OENI_0305) - 293686..294141 (+) 456 WP_032817277.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  E7X43_RS01455 (OENI_0306) - 294141..294611 (+) 471 WP_032817279.1 NUDIX hydrolase -
  E7X43_RS01460 (OENI_0307) radA 294647..296059 (+) 1413 WP_071448846.1 DNA repair protein RadA Machinery gene
  E7X43_RS01465 (OENI_0308) pgmB 296078..296749 (+) 672 WP_011677417.1 beta-phosphoglucomutase -
  E7X43_RS01470 (OENI_0309) rdgB 296845..297459 (+) 615 WP_080485885.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E7X43_RS01475 (OENI_0310) gltX 297482..298978 (+) 1497 WP_071448847.1 glutamate--tRNA ligase -
  E7X43_RS01480 (OENI_0311) - 299100..299918 (+) 819 WP_002817894.1 phosphoribosyltransferase -
  E7X43_RS01485 (OENI_0312) - 299959..300516 (+) 558 WP_071448848.1 hypothetical protein -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 50727.18 Da        Isoelectric Point: 6.5132

>NTDB_id=1116086 E7X43_RS01460 WP_071448846.1 294647..296059(+) (radA) [Oenococcus oeni strain CRBO_1381]
MAKAKTQFVCSNCGYTSARYLGRCPNCGQWNTLIEEKIVPEVVVNRKARISFDGRVAKPKKISEISGNELPRTRTGLREL
NRVLGGGIVPGSLILIGGDPGIGKSTLMLQVSGQLAENGGSVLYVSGEESDNQIKLRADRLGVGSDSFIVYPETDMEQIK
SIVQQIEPDYLVIDSVQTMQEPDLQSPIGSVAQIREVTADLMQIAKTNAITVFIVGHVTKDGSIAGPKILEHMVDTVLYF
EGDANYKYRILRTVKNRFGSTNELGIFEMRDHGLREVANPSEIFLEERLAGATGSAITASMEGSRPILVEVQALVAPTVF
GNAQRVTTGVDRNRVAQILAVLEKHANLLLQNQDAHVRITGGVKIDEPAADLAIALAVASSYHEKATNASDVFLGEVGLG
GEVRSISLIEDRLKEIAKLGFKRAIVSKNNLAGIDLPKGLQVIGVSTLEEALKLGLDLNRASKQKGFADL

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=1116086 E7X43_RS01460 WP_071448846.1 294647..296059(+) (radA) [Oenococcus oeni strain CRBO_1381]
ATGGCAAAAGCAAAAACACAATTTGTTTGTTCCAACTGCGGCTATACCAGTGCTCGCTATCTTGGGCGCTGTCCGAATTG
CGGCCAATGGAATACATTAATAGAGGAAAAAATTGTTCCTGAAGTAGTCGTAAACCGAAAAGCTCGAATCAGTTTCGACG
GGCGGGTTGCCAAACCGAAAAAGATTTCTGAAATCTCCGGTAATGAATTACCTAGAACAAGGACTGGATTAAGGGAATTA
AATCGCGTCCTTGGCGGTGGGATCGTCCCGGGAAGCTTAATTTTGATTGGCGGCGATCCAGGAATCGGGAAATCGACTTT
AATGCTTCAGGTTTCTGGTCAGTTGGCCGAAAATGGCGGAAGTGTTCTTTATGTTTCCGGAGAAGAGTCGGATAATCAGA
TTAAATTGCGTGCTGATCGACTTGGAGTTGGTTCGGATTCTTTTATTGTTTATCCGGAAACGGATATGGAACAAATTAAA
TCAATTGTCCAACAAATCGAACCGGATTATTTGGTAATTGATTCGGTTCAGACGATGCAGGAACCTGATCTGCAGTCGCC
AATTGGCTCGGTTGCCCAAATACGAGAAGTGACGGCCGACTTAATGCAGATCGCCAAAACGAATGCAATTACGGTTTTCA
TTGTCGGGCATGTTACTAAAGATGGATCGATCGCCGGTCCGAAAATTCTCGAGCACATGGTTGATACGGTTCTGTATTTT
GAAGGGGATGCCAATTACAAGTACCGGATCCTCCGAACTGTTAAAAATCGTTTTGGATCGACCAATGAACTTGGTATCTT
TGAAATGCGTGATCATGGTTTACGAGAAGTAGCCAATCCTTCGGAGATCTTTCTCGAAGAGCGTTTGGCCGGCGCAACCG
GATCGGCAATTACAGCCAGTATGGAGGGCAGTCGTCCGATTTTAGTCGAAGTGCAGGCTTTGGTCGCACCAACGGTTTTC
GGTAACGCTCAGCGTGTGACAACCGGCGTTGATCGAAATCGCGTTGCTCAGATTTTAGCCGTTTTGGAAAAACATGCCAA
TCTGCTTTTACAAAATCAGGATGCCCATGTTCGAATTACCGGAGGCGTTAAAATCGACGAGCCGGCAGCTGATTTGGCAA
TTGCTTTAGCCGTTGCCAGTTCTTACCATGAAAAAGCAACTAATGCTTCCGATGTTTTTCTTGGTGAAGTAGGTTTGGGA
GGAGAAGTCCGTTCAATTTCTCTGATTGAGGATCGTTTGAAAGAGATTGCCAAGCTTGGATTTAAACGGGCAATTGTTTC
AAAAAATAATTTGGCAGGAATCGATTTACCAAAAGGTCTGCAGGTGATTGGTGTTTCTACTTTGGAAGAAGCATTGAAAT
TAGGCCTTGATTTGAATAGGGCTTCCAAACAGAAAGGTTTTGCTGATTTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(367-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(76-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N4A8T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

61.81

96.383

0.596
  radA Streptococcus pneumoniae Rx1

61.81

96.383

0.596
  radA Streptococcus pneumoniae D39

61.81

96.383

0.596
  radA Streptococcus pneumoniae TIGR4

61.81

96.383

0.596
  radA Streptococcus mitis NCTC 12261

61.589

96.383

0.594
  radA Streptococcus mitis SK321

61.369

96.383

0.591
  radA Bacillus subtilis subsp. subtilis str. 168

60.613

97.234

0.589

Multiple sequence alignment