Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   E0E10_RS00525 Genome accession   NZ_LR027878
Coordinates   95875..97503 (-) Length   542 a.a.
NCBI ID   WP_001186596.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BPH2003 isolate BPH2003     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 40374..98870 95875..97503 within 0


Gene organization within MGE regions


Location: 40374..98870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E10_RS00175 ccrC 40374..42059 (+) 1686 WP_000676600.1 cassette chromosome recombinase CcrC -
  E0E10_RS00180 tnpA 42107..42592 (+) 486 WP_000093392.1 IS200/IS605 family transposase -
  E0E10_RS00185 - 42884..43222 (+) 339 WP_000175892.1 SAUGI family uracil-DNA glycosylase inhibitor -
  E0E10_RS00195 - 43316..43627 (+) 312 WP_001166716.1 DUF960 family protein -
  E0E10_RS00200 - 43643..44149 (+) 507 WP_000848380.1 DUF1643 domain-containing protein -
  E0E10_RS00205 - 44170..44487 (+) 318 WP_000659046.1 JAB domain-containing protein -
  E0E10_RS00210 - 44606..45691 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  E0E10_RS00215 - 45688..47580 (+) 1893 WP_014532403.1 site-specific integrase -
  E0E10_RS00220 - 47587..47964 (+) 378 WP_001041908.1 DUF6262 family protein -
  E0E10_RS00225 - 48150..48515 (+) 366 WP_000159127.1 ArsR/SmtB family transcription factor -
  E0E10_RS00230 - 48508..50922 (+) 2415 WP_000378486.1 cadmium-translocating P-type ATPase CadA -
  E0E10_RS00235 - 51003..51620 (+) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  E0E10_RS00245 - 51894..52532 (-) 639 Protein_42 sulfite exporter TauE/SafE family protein -
  E0E10_RS00250 cstR 52532..52792 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  E0E10_RS00255 - 52928..53992 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  E0E10_RS00260 cstB 54024..55358 (+) 1335 Protein_45 persulfide dioxygenase-sulfurtransferase CstB -
  E0E10_RS00270 - 55472..56620 (-) 1149 WP_000427807.1 ROK family transcriptional regulator -
  E0E10_RS00275 psm-mec 56940..57008 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  E0E10_RS00280 mecI 57093..57464 (-) 372 Protein_48 mecA-type methicillin resistance repressor MecI -
  E0E10_RS00285 mecR1 57464..59221 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  E0E10_RS00290 mecA 59318..61327 (+) 2010 WP_078056052.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  E0E10_RS00295 - 61373..61801 (-) 429 WP_000872606.1 MaoC family dehydratase -
  E0E10_RS00300 - 61898..62641 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  E0E10_RS00310 - 63678..64067 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  E0E10_RS00315 - 64103..64777 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  E0E10_RS00320 - 64866..65820 (-) 955 Protein_55 replication initiation factor domain-containing protein -
  E0E10_RS00325 mobV 66347..67588 (-) 1242 WP_000122374.1 MobV family relaxase -
  E0E10_RS00330 tet(K) 67777..69156 (-) 1380 WP_000492283.1 tetracycline efflux MFS transporter Tet(K) -
  E0E10_RS00335 - 69353..70027 (+) 675 WP_001106055.1 IS6-like element IS257 family transposase -
  E0E10_RS00340 - 70159..70401 (+) 243 WP_001803415.1 LytTR family DNA-binding domain-containing protein -
  E0E10_RS16705 - 70430..70762 (+) 333 WP_001803416.1 DUF3021 domain-containing protein -
  E0E10_RS00355 - 71020..72192 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  E0E10_RS00360 - 72527..73201 (+) 675 WP_001105988.1 IS6-like element IS257 family transposase -
  E0E10_RS00365 - 73320..73835 (+) 516 WP_000377078.1 hypothetical protein -
  E0E10_RS00375 merB 74030..74680 (-) 651 WP_000790333.1 organomercurial lyase MerB -
  E0E10_RS00380 merA 74762..76404 (-) 1643 Protein_65 mercury(II) reductase -
  E0E10_RS00385 - 76462..76800 (-) 339 WP_000968575.1 hypothetical protein -
  E0E10_RS00390 - 76935..77615 (-) 681 WP_000010351.1 cytochrome c biogenesis CcdA family protein -
  E0E10_RS00395 - 77612..77965 (-) 354 WP_000746201.1 TlpA family protein disulfide reductase -
  E0E10_RS00400 merR 77982..78389 (-) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  E0E10_RS00405 - 78689..80035 (-) 1347 WP_000163428.1 dihydrolipoyl dehydrogenase family protein -
  E0E10_RS00410 - 80318..80992 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  E0E10_RS00415 - 81054..83267 (+) 2214 Protein_72 type I restriction endonuclease subunit R -
  E0E10_RS00420 - 83347..83913 (-) 567 WP_000759939.1 PepSY domain-containing protein -
  E0E10_RS00425 - 84063..84284 (-) 222 WP_001070883.1 hypothetical protein -
  E0E10_RS00430 - 84751..85167 (-) 417 WP_000941331.1 hypothetical protein -
  E0E10_RS00435 - 85229..85756 (-) 528 WP_000717928.1 hypothetical protein -
  E0E10_RS00440 - 85891..86379 (-) 489 WP_000397419.1 hypothetical protein -
  E0E10_RS00445 - 86395..86685 (-) 291 WP_000065262.1 hypothetical protein -
  E0E10_RS00450 - 86742..87614 (-) 873 WP_000373151.1 HTH domain-containing protein -
  E0E10_RS16515 - 87784..87930 (-) 147 Protein_80 JAB domain-containing protein -
  E0E10_RS00460 - 88162..88824 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  E0E10_RS16710 - 89216..89275 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  E0E10_RS00475 erm(A) 89333..90064 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  E0E10_RS00480 - 90190..90972 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  E0E10_RS00485 - 91123..91500 (-) 378 WP_130827602.1 DUF6262 family protein -
  E0E10_RS00490 - 91507..93399 (-) 1893 WP_014532403.1 site-specific integrase -
  E0E10_RS00495 - 93594..93917 (-) 324 WP_000088091.1 JAB domain-containing protein -
  E0E10_RS00500 - 93910..94116 (-) 207 WP_001077283.1 hypothetical protein -
  E0E10_RS00505 - 94118..94639 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  E0E10_RS00510 - 94658..94969 (-) 312 WP_000833212.1 DUF960 family protein -
  E0E10_RS00520 - 95054..95404 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  E0E10_RS00525 ccrB 95875..97503 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  E0E10_RS00530 ccrA 97524..98870 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=1116027 E0E10_RS00525 WP_001186596.1 95875..97503(-) (ccrB) [Staphylococcus aureus strain BPH2003 isolate BPH2003]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPELQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=1116027 E0E10_RS00525 WP_001186596.1 95875..97503(-) (ccrB) [Staphylococcus aureus strain BPH2003 isolate BPH2003]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAAAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865

Multiple sequence alignment