Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   E0E08_RS00480 Genome accession   NZ_LR027873
Coordinates   88862..90208 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus aureus strain BPH2070 isolate BPH2070     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 40375..90208 88862..90208 within 0


Gene organization within MGE regions


Location: 40375..90208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E08_RS00175 ccrC 40375..42051 (+) 1677 WP_000676597.1 cassette chromosome recombinase CcrC -
  E0E08_RS00180 - 42157..42495 (+) 339 WP_000171557.1 SAUGI family uracil-DNA glycosylase inhibitor -
  E0E08_RS00185 - 42591..42902 (+) 312 WP_001083108.1 DUF960 family protein -
  E0E08_RS00190 - 42918..43424 (+) 507 WP_000849085.1 DUF1643 domain-containing protein -
  E0E08_RS00195 - 43445..43762 (+) 318 WP_130825069.1 JAB domain-containing protein -
  E0E08_RS00200 - 43881..44966 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  E0E08_RS00205 - 44963..46855 (+) 1893 WP_001557544.1 site-specific integrase -
  E0E08_RS00210 - 46862..47239 (+) 378 WP_000361059.1 DUF6262 family protein -
  E0E08_RS00215 - 47390..48172 (+) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  E0E08_RS00220 erm(A) 48298..49029 (-) 732 WP_001072197.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  E0E08_RS15635 - 49087..49146 (-) 60 WP_181875734.1 erythromycin resistance leader peptide -
  E0E08_RS00235 - 49538..50200 (+) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  E0E08_RS15590 - 50432..50578 (+) 147 Protein_42 JAB domain-containing protein -
  E0E08_RS00245 - 50748..51620 (+) 873 WP_000373149.1 HTH domain-containing protein -
  E0E08_RS00250 - 51677..51967 (+) 291 WP_000065261.1 hypothetical protein -
  E0E08_RS00255 - 51983..52471 (+) 489 WP_000393586.1 hypothetical protein -
  E0E08_RS00260 - 52606..53133 (+) 528 WP_130825070.1 hypothetical protein -
  E0E08_RS00265 - 53195..53638 (+) 444 WP_000941333.1 hypothetical protein -
  E0E08_RS00270 - 53587..53808 (+) 222 WP_002504703.1 hypothetical protein -
  E0E08_RS00280 - 54201..54524 (+) 324 WP_001176049.1 PepSY domain-containing protein -
  E0E08_RS00285 - 54602..56815 (-) 2214 Protein_50 type I restriction endonuclease subunit R -
  E0E08_RS00290 - 56877..57551 (-) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  E0E08_RS00295 - 57834..59180 (+) 1347 WP_000163428.1 dihydrolipoyl dehydrogenase family protein -
  E0E08_RS00300 merR 59480..59887 (+) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  E0E08_RS00305 - 59904..60257 (+) 354 WP_033842287.1 TlpA family protein disulfide reductase -
  E0E08_RS00310 - 60254..60934 (+) 681 WP_033842288.1 cytochrome c biogenesis CcdA family protein -
  E0E08_RS00315 - 61069..61407 (+) 339 WP_000968575.1 hypothetical protein -
  E0E08_RS00320 merA 61465..63108 (+) 1644 WP_000193219.1 mercury(II) reductase -
  E0E08_RS00325 merB 63190..63840 (+) 651 WP_000790333.1 organomercurial lyase MerB -
  E0E08_RS00335 - 64035..64556 (-) 522 WP_130825071.1 hypothetical protein -
  E0E08_RS00340 - 64590..65264 (-) 675 WP_031917573.1 IS6-like element IS257 family transposase -
  E0E08_RS00345 - 65300..65689 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  E0E08_RS00355 - 66566..67309 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  E0E08_RS00360 - 67406..67834 (+) 429 WP_000872606.1 MaoC family dehydratase -
  E0E08_RS00365 mecA 67880..69889 (-) 2010 WP_130825072.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  E0E08_RS00370 - 69986..71577 (+) 1592 Protein_65 BlaR1 family beta-lactam sensor/signal transducer -
  E0E08_RS00375 mecI 71577..71948 (+) 372 Protein_66 mecA-type methicillin resistance repressor MecI -
  E0E08_RS00380 psm-mec 72033..72101 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  E0E08_RS00385 - 72460..73631 (-) 1172 Protein_68 IS256 family transposase -
  E0E08_RS00390 - 73752..74900 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  E0E08_RS00395 cstB 75014..76348 (-) 1335 Protein_70 persulfide dioxygenase-sulfurtransferase CstB -
  E0E08_RS00400 - 76381..77445 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  E0E08_RS00405 cstR 77581..77841 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  E0E08_RS00410 - 77841..78479 (+) 639 Protein_73 sulfite exporter TauE/SafE family protein -
  E0E08_RS00420 - 78753..79370 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  E0E08_RS00425 - 79451..81865 (-) 2415 WP_116100491.1 cadmium-translocating P-type ATPase CadA -
  E0E08_RS00430 - 81858..82223 (-) 366 WP_000159127.1 ArsR/SmtB family transcription factor -
  E0E08_RS00435 - 82461..82838 (-) 378 WP_001041908.1 DUF6262 family protein -
  E0E08_RS00440 - 82845..84737 (-) 1893 WP_001557544.1 site-specific integrase -
  E0E08_RS00445 - 84932..85255 (-) 324 WP_000088091.1 JAB domain-containing protein -
  E0E08_RS00450 - 85248..85454 (-) 207 WP_001077283.1 hypothetical protein -
  E0E08_RS00455 - 85456..85977 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  E0E08_RS00460 - 85996..86307 (-) 312 WP_000833212.1 DUF960 family protein -
  E0E08_RS00470 - 86392..86742 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  E0E08_RS00475 ccrB 87213..88841 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  E0E08_RS00480 ccrA 88862..90208 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=1115857 E0E08_RS00480 WP_000816499.1 88862..90208(-) (ccrA) [Staphylococcus aureus strain BPH2070 isolate BPH2070]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=1115857 E0E08_RS00480 WP_000816499.1 88862..90208(-) (ccrA) [Staphylococcus aureus strain BPH2070 isolate BPH2070]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725

Multiple sequence alignment