Detailed information NTG1115730

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: TTHNP4_RS09425
• Genome accession: NZ_LR027517
• Coordinates: 1685598..1688267 (+)
• Length: 889 a.a.
• NCBI ID: WP_124105227.1
• Uniprot ID: A0A3P4AU74
• Organism: Thermus thermophilus isolate TTHNAR1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1680598..1693267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TTHNP4_RS09385 (TTHN1_01890)	-	1680695..1681300 (-)	606	Protein_1852	extracellular solute-binding protein	-
TTHNP4_RS09395	-	1681631..1682319 (+)	689	Protein_1853	IS5-like element ISTth7 family transposase	-
TTHNP4_RS09400 (TTHN1_01893)	-	1682424..1683269 (+)	846	WP_124105225.1	transposase	-
TTHNP4_RS09405 (TTHN1_01894)	-	1683338..1683544 (+)	207	WP_011173995.1	cold-shock protein	-
TTHNP4_RS09410 (TTHN1_01895)	surE	1683601..1684356 (+)	756	WP_060384022.1	5'/3'-nucleotidase SurE	-
TTHNP4_RS09415 (TTHN1_01896)	pgeF	1684353..1685081 (+)	729	WP_124105226.1	peptidoglycan editing factor PgeF	-
TTHNP4_RS09420 (TTHN1_01897)	-	1685116..1685601 (+)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
TTHNP4_RS09425 (TTHN1_01898)	pilF	1685598..1688267 (+)	2670	WP_124105227.1	type IV pilus assembly ATPase PilB	Machinery gene
TTHNP4_RS09430 (TTHN1_01899)	-	1688280..1688735 (+)	456	Protein_1860	type IV pilus twitching motility protein PilT	-
TTHNP4_RS09435 (TTHN1_01900)	-	1688827..1689576 (-)	750	WP_252973282.1	transposase	-
TTHNP4_RS09440 (TTHN1_01901)	-	1689618..1690262 (+)	645	Protein_1862	type IV pilus twitching motility protein PilT	-
TTHNP4_RS09445 (TTHN1_01902)	gatB	1690273..1691682 (+)	1410	WP_124105228.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
TTHNP4_RS09450 (TTHN1_01903)	purM	1691688..1692689 (+)	1002	WP_124105229.1	phosphoribosylformylglycinamidine cyclo-ligase	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98016.18 Da
• Isoelectric Point: 5.4637

>NTDB_id=1115730 TTHNP4_RS09425 WP_124105227.1 1685598..1688267(+) (pilF) [Thermus thermophilus isolate TTHNAR1]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSDFLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAAALL
LRSDALRYGAVPIGFRNGKIEVVLSDPRHKETVAQLLKRPARFYLALPQAWEELFGRAYPEKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALKKQRQGGGRLEDTLIQSGKLKPEALAQAVATQLGYPYVDPEEDPP
DPGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAQKQVVEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRICDHCKVEVKPDPEVLRRLGL
SEAEIQGARLSKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=1115730 TTHNP4_RS09425 WP_124105227.1 1685598..1688267(+) (pilF) [Thermus thermophilus isolate TTHNAR1]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAAAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAAA
CCACCAAAAGCGACTTCCTTTACGCCCTGGCCAAGCACTACCCGGAGCTTGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAAAAGGACCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CTTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGAATCGTCCCCGACCCCTCCGCCGCCGCCCTCCTC
CTCCGCTCCGACGCCCTCCGCTACGGCGCCGTGCCCATCGGCTTCCGAAACGGGAAGATAGAGGTGGTCCTCTCCGACCC
ACGGCACAAGGAGACGGTGGCCCAGCTCCTCAAGCGGCCCGCCCGCTTCTACCTCGCCCTTCCCCAGGCCTGGGAGGAGC
TTTTCGGCCGGGCCTACCCCGAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTG
AAGGAAGCCCTGGAAGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAAGAAGCCCTCAAGAAGCAGCGCCAAGGGGGGGGAAGGCTGGAGGACACCCTGATCCAGTCGGGAA
AGCTCAAACCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGATCCCCCC
GATCCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTCTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGGAACCG
CCTCGTCCTCCTGATGAAAGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGCGGAAGGGCCTGA
ACCACGAGGTGGCCCCGGCGGTGGCCACCGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCCAAAAACAGGTGGTGGAAGAGGCTCCAAGTCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAAGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTAAGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCGAAGGGAGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGCCGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTAGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCGC
AGCGCCTGGTGCGTCGGATCTGCGACCACTGCAAGGTGGAGGTGAAGCCGGACCCCGAGGTTCTAAGGCGCCTCGGGCTT
TCCGAAGCCGAAATCCAAGGGGCCAGGCTCTCCAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATTCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAGGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (513-779)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-149)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

3D structure

• AlphaFold DB: A0A3P4AU74

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	97.413	100	0.974
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.618	100	0.557