Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   BAMMD1_RS16750 Genome accession   NZ_LN999829
Coordinates   3513008..3514153 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain B25     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3508008..3519153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMMD1_RS16725 (BAMMD1_3379) - 3508460..3509176 (-) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -
  BAMMD1_RS16730 (BAMMD1_3380) narK 3509274..3510464 (-) 1191 WP_060387223.1 nitrate transporter NarK -
  BAMMD1_RS16735 (BAMMD1_3381) argS 3510599..3512269 (-) 1671 WP_060387224.1 arginine--tRNA ligase -
  BAMMD1_RS16740 (BAMMD1_3382) - 3512266..3512694 (-) 429 WP_060387225.1 DUF1934 domain-containing protein -
  BAMMD1_RS16750 (BAMMD1_3383) rapF 3513008..3514153 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  BAMMD1_RS19010 - 3514137..3514256 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  BAMMD1_RS16755 (BAMMD1_3384) - 3514455..3514658 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  BAMMD1_RS16760 (BAMMD1_3385) - 3514672..3514875 (+) 204 WP_003151062.1 hypothetical protein -
  BAMMD1_RS16765 (BAMMD1_3386) - 3515283..3515564 (+) 282 WP_003151060.1 hypothetical protein -
  BAMMD1_RS16770 (BAMMD1_3387) - 3515608..3516120 (+) 513 WP_003151059.1 stage II sporulation protein M -
  BAMMD1_RS16780 (BAMMD1_3389) - 3516711..3516929 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  BAMMD1_RS16785 (BAMMD1_3390) speB 3517099..3517971 (-) 873 WP_003151054.1 agmatinase -
  BAMMD1_RS16790 (BAMMD1_3391) speE 3518031..3518861 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=1115457 BAMMD1_RS16750 WP_003151067.1 3513008..3514153(+) (rapF) [Bacillus velezensis strain B25]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1115457 BAMMD1_RS16750 WP_003151067.1 3513008..3514153(+) (rapF) [Bacillus velezensis strain B25]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53

Multiple sequence alignment