Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BAMMD1_RS01610 Genome accession   NZ_LN999829
Coordinates   309835..310272 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain B25     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 304835..315272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMMD1_RS19445 - 305417..305581 (-) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -
  BAMMD1_RS01580 (BAMMD1_0309) - 305694..306896 (+) 1203 WP_025853783.1 GTP-binding protein -
  BAMMD1_RS01585 (BAMMD1_0310) - 306961..308097 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  BAMMD1_RS01590 (BAMMD1_0311) - 308112..308546 (+) 435 WP_003156592.1 RDD family protein -
  BAMMD1_RS01595 (BAMMD1_0312) - 308663..308938 (+) 276 WP_369719109.1 YckD family protein -
  BAMMD1_RS19695 - 309042..309125 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  BAMMD1_RS01600 - 309157..309375 (+) 219 Protein_303 family 1 glycosylhydrolase -
  BAMMD1_RS01605 (BAMMD1_0313) nin/comJ 309415..309813 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  BAMMD1_RS01610 (BAMMD1_0314) nucA/comI 309835..310272 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BAMMD1_RS01615 (BAMMD1_0315) hxlB 310631..311188 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  BAMMD1_RS01620 (BAMMD1_0316) hxlA 311185..311820 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  BAMMD1_RS01625 (BAMMD1_0317) - 312052..312414 (+) 363 WP_003156585.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1115393 BAMMD1_RS01610 WP_003156588.1 309835..310272(-) (nucA/comI) [Bacillus velezensis strain B25]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1115393 BAMMD1_RS01610 WP_003156588.1 309835..310272(-) (nucA/comI) [Bacillus velezensis strain B25]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment