Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BN2297_RS23765 Genome accession   NZ_LN879547
Coordinates   5239614..5240999 (-) Length   461 a.a.
NCBI ID   WP_012836620.1    Uniprot ID   A0A5S4SXP0
Organism   Comamonas thiooxydans isolate C19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5234614..5245999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN2297_RS23740 - 5235690..5236454 (+) 765 WP_057094256.1 glycosyltransferase family 25 protein -
  BN2297_RS23745 - 5236444..5237523 (-) 1080 WP_057094258.1 glycosyltransferase -
  BN2297_RS23750 - 5237604..5237813 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  BN2297_RS23755 - 5237958..5238896 (-) 939 WP_046461719.1 branched-chain amino acid transaminase -
  BN2297_RS23760 - 5238978..5239433 (-) 456 WP_012836619.1 hypothetical protein -
  BN2297_RS23765 radA 5239614..5240999 (-) 1386 WP_012836620.1 DNA repair protein RadA Machinery gene
  BN2297_RS23775 - 5241466..5242773 (-) 1308 WP_034363165.1 Glu/Leu/Phe/Val family dehydrogenase -
  BN2297_RS23780 - 5242983..5243906 (+) 924 WP_057094260.1 fumarylacetoacetate hydrolase family protein -
  BN2297_RS23785 - 5244061..5245170 (-) 1110 WP_171832561.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48073.39 Da        Isoelectric Point: 7.6670

>NTDB_id=1115017 BN2297_RS23765 WP_012836620.1 5239614..5240999(-) (radA) [Comamonas thiooxydans isolate C19]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=1115017 BN2297_RS23765 WP_012836620.1 5239614..5240999(-) (radA) [Comamonas thiooxydans isolate C19]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAGGGCT
ATGCGGGCCTGGCCAATGCCCAGCCCGTCACCCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGTGGC
ATTGAGGAGCTGGACCGCGTGCTCGGCGGCGGCGTGGTGGAAGGCGGCGTGGTGCTGATCGGCGGCGACCCCGGAATAGG
TAAGTCCACACTTCTTTTGCAGGCCATGGATGCCTTGCACCGCATCGGCCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GTGGCGCCCAGGTGGCGCTGCGTTCGCGCCGGCTGGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTCGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTGATCGACTCGATCCAGACGGTGTATTCGGACCA
GCTCTCCTCGGCTCCGGGCTCCGTGGCCCAGGTACGCGAATGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTTTGGTGGGCCATGTGACCAAGGACGGTGCGCTGGCCGGCCCACGCGTGCTTGAGCACATGGTGGACACG
GTGCTCTACTTCGAAGGCGACACACACAGCAACTTCCGTCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGTCTCAAGGGCGTGACCAACCCCAGCGCCATCTTCCTGAGCCAGCACAGCG
AACCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACACGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGACCAGCTGCGCGTCGTCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGTGGCGTGCGCATCAATGAGCCTGCAGCTG
ACCTGGCGGTGATGTTGGCAATCACTTCCAGTCTACGTGGAAAGTCGTTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCCGCCCCTCGCGGTCAGGAGCGTCTCAAGGAAGCAGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCCCCAAAGAAGCCCATCGCCGGCCTGACCATCCATGCCGTGGAGCGCGTTGATGAAGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4SXP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus mitis SK321

49.425

94.36

0.466

Multiple sequence alignment