Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BN2297_RS21870 Genome accession   NZ_LN879547
Coordinates   4809948..4811549 (-) Length   533 a.a.
NCBI ID   WP_057093927.1    Uniprot ID   -
Organism   Comamonas thiooxydans isolate C19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4804948..4816549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN2297_RS21845 - 4805119..4805610 (-) 492 WP_003068209.1 putative toxin-antitoxin system toxin component, PIN family -
  BN2297_RS21850 - 4805607..4806905 (-) 1299 WP_057093921.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  BN2297_RS21855 - 4807085..4807423 (+) 339 WP_057093923.1 tRNA-binding protein -
  BN2297_RS21860 - 4807481..4808713 (+) 1233 WP_057093925.1 DUF711 family protein -
  BN2297_RS21865 - 4808849..4809772 (+) 924 WP_003068201.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  BN2297_RS21870 rpoN/rpoN1 4809948..4811549 (-) 1602 WP_057093927.1 RNA polymerase factor sigma-54 Machinery gene
  BN2297_RS21875 lptB 4811695..4812477 (-) 783 WP_003068196.1 LPS export ABC transporter ATP-binding protein -
  BN2297_RS21880 lptA 4812474..4813130 (-) 657 WP_003068194.1 lipopolysaccharide transport periplasmic protein LptA -
  BN2297_RS21885 - 4813261..4813911 (-) 651 WP_003068192.1 thiol:disulfide interchange protein DsbA/DsbL -
  BN2297_RS21890 - 4814082..4814786 (-) 705 WP_046461575.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59077.46 Da        Isoelectric Point: 4.6610

>NTDB_id=1115014 BN2297_RS21870 WP_057093927.1 4809948..4811549(-) (rpoN/rpoN1) [Comamonas thiooxydans isolate C19]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETVGDAPIDVAASASEYSTSDGIHAE
NDAKQTQTAAETIAETPSAETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADMAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMSRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPLRAGSGRVDFSVQLNPEVMPRLRVHDIYASVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDAKKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=1115014 BN2297_RS21870 WP_057093927.1 4809948..4811549(-) (rpoN/rpoN1) [Comamonas thiooxydans isolate C19]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CCGAAACTGTGGGAGATGCTCCTATAGATGTAGCTGCTAGCGCCAGTGAATACAGCACTTCAGATGGTATTCATGCTGAA
AATGATGCGAAGCAAACGCAAACAGCTGCTGAAACAATAGCGGAAACGCCAAGCGCCGAGACTCCGGAAACGCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGGGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGACGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTGCACCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCGGACATGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGATCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGTCTGGAGCCCGTGGGCGTGGGCGCGCGCGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGATCTGATCCGCACGGCCGAGGACGAGGGCACGACCGAGGCGCATATGAGCCGGCTGCTGCT
GGCGCAGGCCATCTGTGCCCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAACAGCTGCGCGAAGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGTCGGCGCTTTGTCGATGTGGAG
CGCCATATCGTGGTGCCCGATGTCATCGTGCGCCCGCTGCGGGCCGGCTCGGGCCGGGTGGACTTCTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCAGCGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAGTTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACCCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCCCAGGGCACGT
TCGAGCTCAAGTATTTCTTCGGCTCGGGCCTGGGTACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAAGAAGCCGCTGTCGGACGCCAAGCTGGCCGACATGCTCAAGGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.311

100

0.514

Multiple sequence alignment