Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   A66_RS08835 Genome accession   NZ_LN847353
Coordinates   1681677..1682501 (-) Length   274 a.a.
NCBI ID   WP_001036790.1    Uniprot ID   A0A0B7KWT3
Organism   Streptococcus pneumoniae strain A66     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1676677..1687501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A66_RS08830 (A66_01758) - 1680055..1681386 (-) 1332 WP_000390049.1 hemolysin family protein -
  A66_RS08835 (A66_01759) endA 1681677..1682501 (-) 825 WP_001036790.1 DNA-entry nuclease EndA Machinery gene
  A66_RS08840 (A66_01760) - 1682540..1682728 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  A66_RS08845 (A66_01761) murA 1682721..1684004 (-) 1284 WP_000358027.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  A66_RS11345 (A66_01762) - 1684028..1684258 (-) 231 WP_000250392.1 DUF1146 family protein -
  A66_RS08850 (A66_01763) sepM 1684316..1685380 (-) 1065 WP_053039757.1 SepM family pheromone-processing serine protease Regulator
  A66_RS08855 (A66_01764) coaD 1685364..1685852 (-) 489 WP_053039758.1 pantetheine-phosphate adenylyltransferase -
  A66_RS08860 (A66_01765) rsmD 1685842..1686381 (-) 540 WP_000706960.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A66_RS08865 (A66_01766) asnA 1686446..1687438 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29949.69 Da        Isoelectric Point: 10.1700

>NTDB_id=1114594 A66_RS08835 WP_001036790.1 1681677..1682501(-) (endA) [Streptococcus pneumoniae strain A66]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=1114594 A66_RS08835 WP_001036790.1 1681677..1682501(-) (endA) [Streptococcus pneumoniae strain A66]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7KWT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982

Multiple sequence alignment