Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   AT694_RS00305 Genome accession   NZ_LN831036
Coordinates   62676..64025 (-) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NCTC13435     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39170..69196 62676..64025 within 0


Gene organization within MGE regions


Location: 39170..69196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT694_RS00170 (ERS445051_00035) mecA 39170..41179 (-) 2010 WP_078067872.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  AT694_RS00175 (ERS445051_00036) - 41276..42262 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  AT694_RS00180 (ERS445051_00037) - 42249..42494 (+) 246 Protein_32 type I restriction enzyme endonuclease domain-containing protein -
  AT694_RS00185 (ERS445051_00038) - 42485..44095 (-) 1611 Protein_33 IS1182 family transposase -
  AT694_RS00190 (ERS445051_00039) - 44144..44650 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  AT694_RS00195 (ERS445051_00040) - 44665..44976 (-) 312 WP_000700851.1 DUF960 family protein -
  AT694_RS00200 (ERS445051_00042) - 45063..45413 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  AT694_RS00205 (ERS445051_00043) - 45830..46645 (-) 816 WP_000164963.1 AAA family ATPase -
  AT694_RS00210 (ERS445051_00044) - 46638..48080 (-) 1443 WP_000790705.1 Mu transposase C-terminal domain-containing protein -
  AT694_RS00215 (ERS445051_00045) - 48052..48645 (-) 594 WP_000690628.1 recombinase family protein -
  AT694_RS00220 (ERS445051_00046) - 48852..49433 (+) 582 WP_001124173.1 TetR/AcrR family transcriptional regulator -
  AT694_RS14225 - 49717..51321 (+) 1605 Protein_41 IS1182-like element IS1182 family transposase -
  AT694_RS00235 (ERS445051_00049) - 51448..51693 (+) 246 WP_000567888.1 single-stranded DNA-binding protein -
  AT694_RS00240 (ERS445051_00050) - 51796..52665 (+) 870 WP_000228166.1 nucleotidyltransferase domain-containing protein -
  AT694_RS00245 (ERS445051_00051) - 52646..53380 (+) 735 WP_000662263.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  AT694_RS00250 (ERS445051_00052) - 53413..54321 (+) 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
  AT694_RS00255 (ERS445051_00053) sat4 54318..54860 (+) 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
  AT694_RS00260 (ERS445051_00054) aph(3')-IIIa 54953..55747 (+) 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  AT694_RS15150 - 56023..56154 (+) 132 Protein_48 HTH domain-containing protein -
  AT694_RS15155 - 56230..56975 (+) 746 Protein_49 helix-turn-helix transcriptional regulator -
  AT694_RS00275 (ERS445051_00057) - 57086..57745 (+) 660 WP_000802831.1 dihydrofolate reductase family protein -
  AT694_RS14240 - 58466..60070 (-) 1605 Protein_51 IS1182-like element IS1182 family transposase -
  AT694_RS00295 (ERS445051_00060) - 60229..60660 (+) 432 Protein_52 hypothetical protein -
  AT694_RS14875 - 60661..60744 (+) 84 Protein_53 ATP-binding protein -
  AT694_RS00300 (ERS445051_00061) ccrB 61026..62654 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  AT694_RS00305 (ERS445051_00062) ccrA 62676..64025 (-) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  AT694_RS00310 (ERS445051_00063) cch1 64259..66052 (-) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  AT694_RS00315 (ERS445051_00064) ssb 66052..66348 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  AT694_RS00320 (ERS445051_00065) - 66541..67587 (-) 1047 WP_001042023.1 hypothetical protein -
  AT694_RS00325 (ERS445051_00066) - 68042..69196 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=1114294 AT694_RS00305 WP_000815640.1 62676..64025(-) (ccrA) [Staphylococcus aureus strain NCTC13435]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1114294 AT694_RS00305 WP_000815640.1 62676..64025(-) (ccrA) [Staphylococcus aureus strain NCTC13435]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759

Multiple sequence alignment