Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BN1209_RS00620 Genome accession   NZ_LN794158
Coordinates   112532..114013 (-) Length   493 a.a.
NCBI ID   WP_045750499.1    Uniprot ID   -
Organism   Candidatus Methylopumilus turicensis strain MMS-10A-171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 107532..119013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN1209_RS00590 ptsP 107959..109682 (-) 1724 Protein_108 phosphoenolpyruvate--protein phosphotransferase -
  BN1209_RS00595 (BN1209_0119) - 109682..109948 (-) 267 WP_045750494.1 HPr family phosphocarrier protein -
  BN1209_RS00600 (BN1209_0120) - 109945..110370 (-) 426 WP_045750495.1 PTS sugar transporter subunit IIA -
  BN1209_RS00605 (BN1209_0121) rapZ 110367..111206 (-) 840 WP_045750496.1 RNase adapter RapZ -
  BN1209_RS00610 (BN1209_0122) hprK 111206..112156 (-) 951 WP_045750497.1 HPr(Ser) kinase/phosphatase -
  BN1209_RS00615 (BN1209_0123) hpf 112175..112501 (-) 327 WP_045750498.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BN1209_RS00620 (BN1209_0124) rpoN/rpoN1 112532..114013 (-) 1482 WP_045750499.1 RNA polymerase factor sigma-54 Machinery gene
  BN1209_RS00625 (BN1209_0125) lptB 114043..114765 (-) 723 WP_045750500.1 LPS export ABC transporter ATP-binding protein -
  BN1209_RS00630 (BN1209_0126) lptA 114775..115308 (-) 534 WP_052661060.1 lipopolysaccharide transport periplasmic protein LptA -
  BN1209_RS00635 (BN1209_0127) lptC 115319..116050 (-) 732 WP_045750502.1 LPS export ABC transporter periplasmic protein LptC -
  BN1209_RS00640 (BN1209_0128) - 116082..116543 (-) 462 WP_231855154.1 KdsC family phosphatase -
  BN1209_RS00645 (BN1209_0129) - 116624..117622 (-) 999 WP_045751865.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 56041.51 Da        Isoelectric Point: 5.2796

>NTDB_id=1114013 BN1209_RS00620 WP_045750499.1 112532..114013(-) (rpoN/rpoN1) [Candidatus Methylopumilus turicensis strain MMS-10A-171]
MKQSLQFKLSQNLALTPQLQQSIRLLQLSSQELNQELETILQENPLLERTDKNDDGSDNINEFSIEGFATADSVVSITPA
QTNEQNQNESAFQDSLSDDYFGGTSNHRWEENNHADEDIDYTFQEVVVPSLREHLISQLHLMPLSDRDQLLSMVLIDAIN
EDGYLEQSLEEIAEILNAELEVDMLELETALKHIQHLDPPGVGARNLSECLTLQLKLLPNELREENKNVFDLALQMAEQH
LPSLASRDFAKLKKNLRCDDESLRLAQKMLKHLDPRPGSEFTKLASGHYIQHEIMVKKVKGQWLASLNDEVVPKLRINKL
YADILKRNRDSSSQYLNGQMQEAKWMIKNIEQRFSTILRVSQAIIDQQSQFFEHGEIAMRPLILREIADALGLHESTVSR
VTTRKYMLTPRGVYELKYFFGSHVSTNTGGACSATAIRALIKQLVAEENPKKPFSDNQLTAVLSKQGIVVARRTIAKYRE
SLNIPAANQRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=1114013 BN1209_RS00620 WP_045750499.1 112532..114013(-) (rpoN/rpoN1) [Candidatus Methylopumilus turicensis strain MMS-10A-171]
ATGAAACAAAGCTTACAATTCAAACTCTCACAAAACTTAGCGCTAACCCCGCAACTGCAGCAGTCTATTAGACTCCTCCA
GCTTTCATCACAAGAGCTCAATCAAGAGCTTGAAACCATCTTGCAAGAAAACCCATTGCTCGAACGCACGGATAAAAATG
ACGATGGGTCAGACAATATAAACGAATTCAGCATAGAAGGGTTTGCGACAGCAGATTCAGTGGTTAGCATTACTCCTGCC
CAAACAAACGAGCAAAATCAGAACGAAAGCGCATTTCAAGACTCATTAAGCGATGATTACTTTGGTGGAACCAGCAATCA
TCGTTGGGAAGAAAACAACCATGCTGATGAAGATATCGACTACACATTTCAAGAAGTCGTTGTACCTTCCTTGCGCGAAC
ACCTCATCAGTCAACTTCATTTAATGCCGCTCTCAGATCGAGATCAACTGCTTTCAATGGTGCTCATTGATGCGATTAAT
GAAGATGGATACCTAGAGCAATCTCTCGAAGAAATCGCTGAAATTCTCAACGCAGAACTTGAAGTGGACATGCTGGAACT
TGAAACTGCACTCAAACACATACAACACCTTGACCCACCAGGCGTGGGGGCTAGAAACTTATCCGAATGCCTCACATTGC
AACTCAAGCTGCTGCCAAATGAACTGCGAGAAGAGAATAAAAACGTGTTTGATTTAGCCCTGCAAATGGCGGAGCAACAT
TTACCATCACTCGCGTCACGCGACTTTGCCAAACTGAAAAAAAACCTCCGTTGCGATGATGAGAGCCTTCGCTTAGCCCA
GAAAATGCTTAAGCATCTCGATCCGCGCCCAGGAAGTGAATTCACAAAACTCGCCTCAGGTCATTATATTCAACATGAAA
TTATGGTGAAAAAAGTCAAAGGGCAATGGCTTGCAAGTTTGAATGATGAGGTCGTTCCTAAATTACGTATTAATAAGCTC
TATGCCGATATTCTGAAACGTAATCGAGATAGCTCAAGCCAATATTTAAATGGCCAAATGCAAGAAGCAAAATGGATGAT
TAAAAATATTGAGCAACGGTTCTCCACGATTTTAAGAGTGTCTCAGGCAATCATTGACCAACAAAGTCAGTTCTTCGAAC
ATGGCGAGATTGCAATGCGACCCTTGATACTCCGTGAAATTGCAGATGCACTTGGTTTACACGAATCAACTGTCTCACGT
GTAACGACGCGAAAGTACATGCTCACACCACGTGGCGTTTACGAGTTAAAATATTTTTTTGGAAGCCATGTCTCGACAAA
TACAGGTGGCGCATGTTCAGCAACAGCCATTCGAGCTCTGATTAAACAATTGGTGGCAGAAGAAAACCCAAAAAAACCTT
TTTCAGATAATCAACTAACTGCAGTATTATCAAAACAAGGCATTGTCGTTGCAAGACGTACAATCGCTAAGTACCGCGAG
TCTCTTAACATACCTGCGGCCAACCAACGCAAGTCCTTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-491)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.784

100

0.525

Multiple sequence alignment