Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNRMP_RS13355 Genome accession   NZ_JBLWDC010000001
Coordinates   2878367..2879866 (-) Length   499 a.a.
NCBI ID   WP_020831172.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PD 7123     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2873367..2884866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNRMP_RS13325 (ACNRMP_13325) - 2873875..2874525 (-) 651 WP_028852569.1 LON peptidase substrate-binding domain-containing protein -
  ACNRMP_RS13330 (ACNRMP_13330) rapZ 2874603..2875493 (-) 891 WP_028852570.1 RNase adapter RapZ -
  ACNRMP_RS13335 (ACNRMP_13335) - 2875587..2875922 (-) 336 WP_020425146.1 PsiF family protein -
  ACNRMP_RS13340 (ACNRMP_13340) hprK 2876113..2877087 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  ACNRMP_RS13345 (ACNRMP_13345) ptsN 2877158..2877613 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACNRMP_RS13350 (ACNRMP_13350) hpf 2877962..2878315 (-) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACNRMP_RS13355 (ACNRMP_13355) rpoN/rpoN1 2878367..2879866 (-) 1500 WP_020831172.1 RNA polymerase factor sigma-54 Machinery gene
  ACNRMP_RS13360 (ACNRMP_13360) lptB 2879964..2880752 (-) 789 WP_019717491.1 LPS export ABC transporter ATP-binding protein -
  ACNRMP_RS13365 (ACNRMP_13365) lptA 2880786..2881361 (-) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  ACNRMP_RS13370 (ACNRMP_13370) lptC 2881430..2882062 (-) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  ACNRMP_RS13375 (ACNRMP_13375) - 2882064..2882651 (-) 588 WP_011000373.1 KdsC family phosphatase -
  ACNRMP_RS13380 (ACNRMP_13380) - 2882690..2883673 (-) 984 WP_028852571.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55213.53 Da        Isoelectric Point: 4.8037

>NTDB_id=1113382 ACNRMP_RS13355 WP_020831172.1 2878367..2879866(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain PD 7123]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTADASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=1113382 ACNRMP_RS13355 WP_020831172.1 2878367..2879866(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain PD 7123]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGTGCGGACCATGCCGACACAGCCGACGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998

Multiple sequence alignment