Detailed information    

insolico Bioinformatically predicted

Overview

Name   sepM   Type   Regulator
Locus tag   V4T04_RS13065 Genome accession   NZ_JAZICS010000016
Coordinates   19017..20045 (+) Length   342 a.a.
NCBI ID   WP_003132657.1    Uniprot ID   S6FVM6
Organism   Lactococcus lactis subsp. lactis strain DGCC12653     
Function   processing of CSP (predicted from homology)   
Competence regulation

Genomic Context

Location: 14017..25045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4T04_RS13040 - 14565..15059 (-) 495 WP_012898649.1 RNA polymerase sigma factor -
  V4T04_RS13045 lmrP 15247..16473 (+) 1227 WP_012898648.1 multidrug efflux MFS transporter LmrP -
  V4T04_RS13050 - 16556..17776 (+) 1221 WP_003132654.1 MDR family MFS transporter -
  V4T04_RS13055 rsmD 17946..18485 (+) 540 WP_015427191.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  V4T04_RS13060 coaD 18533..19030 (+) 498 WP_010906348.1 pantetheine-phosphate adenylyltransferase -
  V4T04_RS13065 sepM 19017..20045 (+) 1029 WP_003132657.1 SepM family pheromone-processing serine protease Regulator
  V4T04_RS13070 - 20166..21023 (+) 858 WP_349652199.1 transcriptional regulator -
  V4T04_RS13075 - 21126..22052 (+) 927 WP_349652200.1 radical SAM protein -
  V4T04_RS13080 - 22049..23248 (+) 1200 WP_223247198.1 hypothetical protein -
  V4T04_RS13085 - 23241..24068 (+) 828 WP_010906344.1 radical SAM protein -
  V4T04_RS13090 - 24065..24274 (+) 210 WP_003132661.1 hypothetical protein -
  V4T04_RS13095 - 24261..24758 (+) 498 WP_021214977.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37349.96 Da        Isoelectric Point: 9.9183

>NTDB_id=1113277 V4T04_RS13065 WP_003132657.1 19017..20045(+) (sepM) [Lactococcus lactis subsp. lactis strain DGCC12653]
MKKNKKINPKLKWGISIGLIVVALLALFYPTSYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYSH
FNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAIYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELADTITAV
NGQQFTSSADMIAYVSKQKVGDSVTIEYTRIDGTKHKSTGKYIKIANGKTGIGISLVDHTEVVTTPKVTVNAGSIGGPSA
GMMFTLEIYSQLTGKDLRNGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAKK
LKTKMKIVPVKTIQDALDYLEK

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=1113277 V4T04_RS13065 WP_003132657.1 19017..20045(+) (sepM) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGAAAAAAAATAAAAAAATTAATCCCAAATTAAAATGGGGAATCTCGATTGGTCTAATTGTTGTTGCCCTTTTAGCTTT
ATTTTATCCAACGAGTTACTATGTGGAAATGCCCGGAACAACCGAGCCTTTAGGGAAAATGGTCAAAGTTGAAGGCAAAA
AAGATGAACATAAAGGTGATTTTTTTCTGACGACTGTCCAAATTGCACGAGCAAATCTTGCTACAATGATTTACAGCCAT
TTTAATAGTTTTACAAGCATTTACAGTGAGCAAGAAATGACTGGTGGACTTAACGACGCACAGTTCAACCGTGTCAACCA
GTTTTACATGGAAACGGCACAGAATACAGCGATTTATCAAGCTTTTAAATTAGCTAATAAACCTTATGAACTTAAATATG
AAGGCGTTTATGTCCTTGATATTGCCAAAAATTCGACCTTTAAAAATAAGCTAGAACTTGCAGATACGATTACAGCAGTC
AATGGTCAACAGTTTACATCAAGTGCCGACATGATTGCTTATGTTTCCAAACAAAAAGTCGGTGATTCAGTAACGATTGA
ATACACGAGAATTGACGGCACAAAGCACAAGTCAACTGGCAAATACATCAAAATTGCTAATGGTAAAACAGGAATAGGGA
TTAGCCTAGTTGACCATACTGAAGTCGTGACGACTCCGAAAGTTACTGTCAACGCGGGTTCAATCGGTGGTCCCTCTGCG
GGGATGATGTTTACACTAGAAATTTACAGTCAGTTGACAGGAAAAGATTTGCGAAACGGTCGCGAAATTGCTGGAACTGG
GACGATTGAACACGACGGAAGCATTGGTCAAATCGGCGGTGTTGATAAAAAAGTAGCCACAGCGAGTAAAGAAGGGGCAA
AAGTCTTCTTAGTGCCTGATTCAGGCACCAAAAAGGAAAGTAGCAACAATTACCTTGGAGCCAAAGCCGCCGCTAAAAAA
CTAAAAACGAAGATGAAAATCGTTCCCGTCAAAACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(134-194)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(227-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6FVM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sepM Streptococcus mutans UA159

48.696

100

0.491

Multiple sequence alignment