Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MWH27_RS18960 Genome accession   NZ_JALJVV010000001
Coordinates   3519542..3520933 (-) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus subtilis strain A-5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3514542..3525933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWH27_RS18925 (MWH27_18870) flgL 3514714..3515610 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  MWH27_RS18930 (MWH27_18875) flgK 3515621..3517144 (-) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  MWH27_RS18935 (MWH27_18880) flgN 3517163..3517645 (-) 483 WP_014481048.1 flagellar protein FlgN -
  MWH27_RS18940 (MWH27_18885) flgM 3517661..3517927 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  MWH27_RS18945 (MWH27_18890) yvyF 3518008..3518427 (-) 420 WP_014481050.1 TIGR03826 family flagellar region protein -
  MWH27_RS18950 (MWH27_18895) comFC 3518500..3519222 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  MWH27_RS18955 (MWH27_18900) comFB 3519186..3519482 (-) 297 WP_003227989.1 late competence protein ComFB -
  MWH27_RS18960 (MWH27_18905) comFA 3519542..3520933 (-) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  MWH27_RS18965 (MWH27_18910) fakBA 3521040..3521885 (-) 846 WP_014481053.1 DegV family protein -
  MWH27_RS18970 (MWH27_18915) degU 3521983..3522672 (-) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  MWH27_RS18975 (MWH27_18920) degS 3522755..3523912 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  MWH27_RS18980 (MWH27_18925) - 3524129..3524782 (+) 654 WP_014481055.1 YigZ family protein -
  MWH27_RS18985 (MWH27_18930) - 3524782..3525774 (+) 993 WP_014481056.1 LCP family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=1113044 MWH27_RS18960 WP_014481052.1 3519542..3520933(-) (comFA) [Bacillus subtilis strain A-5]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=1113044 MWH27_RS18960 WP_014481052.1 3519542..3520933(-) (comFA) [Bacillus subtilis strain A-5]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981

Multiple sequence alignment